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Thermo Scientific™
Phusion™ Plus DNA Polymerase
Thermo Scientific Phusion Plus DNA Polymerase is a hot-start, high-fidelity DNA polymerase that brings together protein fusion technology and universal primer annealing.
製品番号（カタログ番号） F630S
価格（JPY）お問い合わせください ›
12,400
온라인 행사
Ends: 26-Dec-2025
20,700割引額 8,300 (40%)
Each
反応数:
反応100回分
Thermo Scientific Phusion Plus DNA Polymerase is a hot-start, high-fidelity DNA polymerase that brings together protein fusion technology and universal primer annealing. It is a Pyrococcus-like proofreading DNA polymerase fused to a processivity-enhancing domain, enabling Phusion Plus DNA Polymerase to generate PCR sequences with high accuracy, sensitivity, and inhibitor tolerance.
Features include:
- >100X higher sequence accuracy than Taq polymerase
- Universal annealing temperature of 60°C in PCR
- Hot-start mechanism reduces non-specific amplification and primer degradation
- Automation enabled due to 24-hour stability of assembled reactions
- High inhibitor tolerance for work with DNA of suboptimal purity
The novel Phusion Plus reaction buffer includes proprietary additives that stabilize primer/template duplexes during the annealing step at 60°C. This unique buffer formulation increases PCR specificity without the need to optimize annealing temperature for each primer pair, helping save time and avoid mistakes in PCR runs. Furthermore, with Phusion Plus DNA Polymerase and its universal annealing feature, different PCR reactions can be cycled together using the same protocol.
Phusion Plus DNA Polymerase is inactive at ambient temperatures due to its hot-start mechanism mediated by Affibody molecules, allowing setup and storage of assembled PCR reactions at room temperature. Enzyme activity is fully restored after the initial denaturation step.
Applications
- High-fidelity PCR
- Cloning & mutagenesis
- Template generation for sequencing
- GC-rich PCR
- Long-range PCR (up to 20 kb)
- High-throughput PCR
Notes
- To simplify reactrion setup, Phusion Plus DNA Polymerase is also available in ready-to-use master mix formats.
研究用途にのみご使用ください。診断目的には使用できません。
仕様
フィデリティ（vs. Taq）>100X
ホットスタート内蔵ホットスタート
反応数反応100回分
オーバーハングBlunt
ポリメラーゼPhusion Plus DNA Polymerase
数量100 x 50 μL reactions
反応形態Separate components
出荷条件Room Temp or Wet Ice
サイズ（最終製品）20 kb or less
使用対象（アプリケーション）Hot-start PCR, High-fidelity PCR
GC-Rich PCR Performance高
反応速度15 to 30 sec./kb
Unit SizeEach
組成および保存条件
• Phusion Plus DNA Polymerase (50 μL)
• 5X Phusion Plus Buffer (1.25 mL)
• 5X Phusion GC Enhancer (1.25 mL)
Store at –15°C to –25°C.
• 5X Phusion Plus Buffer (1.25 mL)
• 5X Phusion GC Enhancer (1.25 mL)
Store at –15°C to –25°C.