FluoSpheres™ Carboxylate-Modified Microspheres
FluoSpheres™ Carboxylate-Modified Microspheres
Citations & References (9)
Invitrogen™

FluoSpheres™ Carboxylate-Modified Microspheres

各種の色および粒子サイズを揃えたカルボン酸修飾FluoSphereミクロスフェアを使用することで、もっとも明るい蛍光が得られます。
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製品番号（カタログ番号）直径（メートル法）色励起／発光数量
F87930.04 μmRed580/605 nm1 mL
F88030.1 μm黄-緑505/515 nm10 mL
F88161.0 μmクリムゾン625/645 nm2 mL
F88231.0 μm黄-緑505/515 nm10 mL
F88010.1 μmRed580/605 nm10 mL
F88110.2 μm黄緑色505/515 nm10 mL
F88070.2 μm暗赤色660/680 nm2 mL
F107200.04 μm黄～緑, オレンジ, 赤, ダークレッド505/515、540/560、580/605、660/680 nm1 mL（各）
F208810.2 μmオレンジ365/610 nm2 mL
F8783
または、製品番号F-8783
0.02 μm暗赤色660/680 nm2 mL
F87860.02 μmRed580/605 nm10 mL
F8795
または、製品番号F-8795
0.04 μm黄緑色505/515 nm1 mL
F88130.5 μm黄緑色505/515 nm10 mL
F88201.0 μmオレンジ540/560 nm10 mL
F88252.0 μmナイルレッド535/575 nm2 mL
F88272.0 μm黄-緑505/515 nm2 mL
F8781
または、製品番号F-8781
0.02 μm青色365/415 nm10 mL
F8782
または、製品番号F-8782
0.02 μmクリムゾン625/645 nm2 mL
F87840.02 μmナイルレッド535/575 nm10 mL
F87870.02 μm黄-緑505/515 nm10 mL
F8789
または、製品番号F-8789
0.04 μm暗赤色660/680 nm1 mL
F8792
または、製品番号F-8792
0.04 μmオレンジ540/560 nm1 mL
F87940.04 μm赤色～オレンジ565/580 nm1 mL
F87970.1 μm青色350/440 nm10 mL
F87990.1 μm赤外線715/755 nm1 mL
F8800
または、製品番号F-8800
0.1 μmオレンジ540/560 nm10 mL
F88050.2 μm青色365/415 nm10 mL
F88060.2 μmクリムゾン625/645 nm2 mL
F88090.2 μmオレンジ540/560 nm10 mL
F88100.2 μmRed580/605 nm10 mL
F88120.5 μmRed580/605 nm10 mL
F88141.0 μm青色365/415 nm10 mL
F88151.0 μm青色350/440 nm10 mL
F88191.0 μmナイルレッド535/575 nm10 mL
F88211.0 μmRed580/605 nm10 mL
F8824
または、製品番号F-8824
2.0 μm青色365/415 nm2 mL
F88262.0 μmRed580/605 nm2 mL
製品番号（カタログ番号） F8793
価格（JPY）
95,100
Each
お問い合わせください ›
直径（メートル法）:
0.04 μm
色:
Red
励起／発光:
580/605 nm
数量:
1 mL

フローサイトメトリー、顕微鏡、HTS、HCS、免疫測定法、当社の多様なFluoSpheresカルボン酸修飾ミクロスフェアを用いるその他のラボラトリー用途にも対応します。FluoSphereビーズは、粒子捕捉アプリケーションとして、タンパク質、核酸、生体分子の受動的吸着または能動的な共有結合に使用することができます。FluoSphereミクロスフェアには独自の蛍光色素が添加されており、もっとも明るいミクロスフェアとして使用できます。

当社のCarboxylate-Modified FluoSphere Microspheres（カルボン酸修飾FluoSphereミクロスフェア）は、ポリスチレンミクロスフェアから製造され、さまざまな独自の色素で充填されており、蛍光顕微鏡、フローサイトメトリー、HTS、HCS、細胞トレーシングなどのラボラトリー用のもっとも明るい蛍光を可視化します。特殊な染色法を使用することで、ビーズ表面ではなく各ポリスチレンミクロスフェア内に、すべての蛍光色素分子を収容できます。こうしたビーズ内での保護環境は、光退色などの有害な環境影響から色素を保護します。当社のカルボン酸修飾ミクロスフェアは、リガンドの共有結合性を実現するために、複数のカルボン酸を含んだ親水性ポリマーでコーティングされています。さまざまな研究用途や実験に使用できるように、各サイズの粒径をご用意しています。
For Research Use Only. Not for use in diagnostic procedures.
仕様
励起／発光580/605 nm
製品ラインFLUOSPHERES
数量1 mL
表面修飾カルボン酸塩
色Red
直径（メートル法）0.04 μm
使用対象（アプリケーション）蛍光顕微鏡法
材料ポリスチレン
製品タイプカルボン酸修飾ミクロスフェア
Unit SizeEach
組成および保存条件
冷蔵庫（2～8℃）に保存し、遮光。

よくあるご質問（FAQ）

I read that carboxylate-modified latex (CML) beads have a "fluffy coat" of carboxyl groups on their surface. What is meant by this?

The CML beads have a high density of carboxyl groups at the surface. The surface layer is quite hydrophilic and at the appropriate pH (pH>5), are charged; due to electrostatic repulsion, this type of surface is 3-dimensional and may be considered analogous to the fuzz on a tennis ball.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

I have some FluoSpheres polystyrene microspheres, with 20 nm diameter. They are aggregating a lot. What can I do about it?

The smaller the microspheres, the greater the propensity to aggregate. But the aggregation is not irreversible. Sonicate in a bath sonicator or vortex to disperse, just prior to use. You can also add a small concentration of Tween-20 or Triton X-100 (unless you are using them in a live-cell system).

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

I sonicated my 2.0 µm carboxylate-modified microspheres, as recommended, but saw foaming (bubbles) on top of the solution. Should I be concerned?

Use of a bath sonicator is recommended to help break up any aggregated microspheres. The foaming is from Tween-20, which is in the stock solution to help prevent aggregation. It is normal and expected to see bubbles from this. Do not use a probe sonicator, which would cause damage to the microspheres (as well as much more bubbling).

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

What is the warranty for FluoSpheres microspheres?

The warranty period for FluoSpheres microspheres is 1-year from the date of shipment.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

After washing and centrifugation, there was only a very small pellet left of my microsphere beads and the solution was transparent. Why is this?

Centrifugation is not an effective way to collect smaller microspheres; many particles remain in the solution even if you can visualize a small pellet. For beads less than 1 µm in diameter, we recommend washing by either:

Cross-flow filtration, as these particles have a very high compression modulus and can withstand high g-forces without risk of harm or dialysis with a 500 kDa MWCO
Note: Microspheres greater than 1 µm in diameter can be centrifuged at 1,300 rpm.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

View all FluoSpheres™ Carboxylate-Modified Microspheres FAQs

引用および参考文献 (9)

引用および参考文献
Abstract
Distribution of retrogradely transported fluorescent latex microspheres in rat lumbosacral ventral root axons following peripheral crush injury: a light and electron microscopic study.
Authors:Persson HG, Gatzinsky KP
Journal:Brain Res
PubMed ID:8118679
'The retrograde axonal transport of fluorescent latex microspheres, which are tracers extensively used for studying the neuronal connectivity in the CNS, was investigated in large myelinated lumbosacral ventral root nerve fibres of adult rats following peripheral crush injury. After crushing the sciatic nerve, a suspension of 30 nm red-fluorescent latex ... More
Identification of catecholaminergic inputs to and outputs from aromatase-containing brain areas of the Japanese quail by tract tracing combined with tyrosine hydroxylase immunocytochemistry.
Authors:Balthazart J, Absil P
Journal:J Comp Neurol
PubMed ID:9183702
'In the quail brain, aromatase-immunoreactive (ARO-ir) neurons located in the medial preoptic nucleus (POM) and caudal paleostriatum ventrale/nucleus accumbens/nucleus striae terminalis complex (PVT/nAc/nST) receive catecholaminergic inputs identified by the presence of tyrosine hydroxylase-immunoreactive (TH-ir) fibers and punctate structures. The origin of these inputs was analyzed by retrograde tracing with cholera ... More
The origins of catecholaminergic innervation in the rostral ventromedial medulla oblongata of the rat.
Authors:Tanaka M, Matsumoto Y, Murakami T, Hisa Y, Ibata Y
Journal:Neurosci Lett
PubMed ID:8710209
'The localization of catecholaminergic neuronal cell bodies which project to the rostral ventromedial medulla oblongata (RVM) were investigated by the combined technique with dopamine beta-hydroxylase (DBH), phenylethanolamine N-methyltransferase (PNMT) or tyrosine hydroxylase (TH) immunocytochemistry and retrograde neuronal tracing method using fluorescent latex microspheres (FluoSpheres) injected into the center of the ... More
Optimized localization analysis for single-molecule tracking and super-resolution microscopy.
Authors:Mortensen KI, Churchman LS, Spudich JA, Flyvbjerg H,
Journal:Nat Methods
PubMed ID:20364147
'We optimally localized isolated fluorescent beads and molecules imaged as diffraction-limited spots, determined the orientation of molecules and present reliable formulas for the precision of various localization methods. Both theory and experimental data showed that unweighted least-squares fitting of a Gaussian squanders one-third of the available information, a popular formula ... More
T-type channels control the opioidergic descending analgesia at the low threshold-spiking GABAergic neurons in the periaqueductal gray.
Authors:Park C, Kim JH, Yoon BE, Choi EJ, Lee CJ, Shin HS,
Journal:Proc Natl Acad Sci U S A
PubMed ID:20682748
'Endogenous opioids generate analgesic signals in the periaqueductal gray (PAG). However, because cell types in the PAG are difficult to identify, its neuronal mechanism has remained poorly understood. To address this issue, we characterized PAG neurons by their electrical properties using differentially labeled GABAergic and output neurons in the PAG. ... More
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