FluoSpheres™ Carboxylate-Modified Microspheres

Citations & References (2)

Invitrogen™

FluoSpheres™ Carboxylate-Modified Microspheres

Name: FluoSpheres™ Carboxylate-Modified Microspheres
SKU: F8806

各種の色および粒子サイズを揃えたカルボン酸修飾FluoSphereミクロスフェアを使用することで、もっとも明るい蛍光が得られます。

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製品番号（カタログ番号）	直径（メートル法）	色	励起／発光	数量
F8806	0.2 μm	クリムゾン	625/645 nm	2 mL
F8803	0.1 μm	黄-緑	505/515 nm	10 mL
F8816	1.0 μm	クリムゾン	625/645 nm	2 mL
F8823	1.0 μm	黄-緑	505/515 nm	10 mL
F8801	0.1 μm	Red	580/605 nm	10 mL
F8811	0.2 μm	黄緑色	505/515 nm	10 mL
F8807	0.2 μm	暗赤色	660/680 nm	2 mL
F10720	0.04 μm	黄～緑, オレンジ, 赤, ダークレッド	505/515、540/560、580/605、660/680 nm	1 mL（各）
F20881	0.2 μm	オレンジ	365/610 nm	2 mL
F8783 または、製品番号F-8783	0.02 μm	暗赤色	660/680 nm	2 mL
F8786	0.02 μm	Red	580/605 nm	10 mL
F8795 または、製品番号F-8795	0.04 μm	黄緑色	505/515 nm	1 mL
F8813	0.5 μm	黄緑色	505/515 nm	10 mL
F8820	1.0 μm	オレンジ	540/560 nm	10 mL
F8825	2.0 μm	ナイルレッド	535/575 nm	2 mL
F8827	2.0 μm	黄-緑	505/515 nm	2 mL
F8781 または、製品番号F-8781	0.02 μm	青色	365/415 nm	10 mL
F8782 または、製品番号F-8782	0.02 μm	クリムゾン	625/645 nm	2 mL
F8784	0.02 μm	ナイルレッド	535/575 nm	10 mL
F8787	0.02 μm	黄-緑	505/515 nm	10 mL
F8789 または、製品番号F-8789	0.04 μm	暗赤色	660/680 nm	1 mL
F8792 または、製品番号F-8792	0.04 μm	オレンジ	540/560 nm	1 mL
F8793	0.04 μm	Red	580/605 nm	1 mL
F8794	0.04 μm	赤色～オレンジ	565/580 nm	1 mL
F8797	0.1 μm	青色	350/440 nm	10 mL
F8799	0.1 μm	赤外線	715/755 nm	1 mL
F8800 または、製品番号F-8800	0.1 μm	オレンジ	540/560 nm	10 mL
F8805	0.2 μm	青色	365/415 nm	10 mL
F8809	0.2 μm	オレンジ	540/560 nm	10 mL
F8810	0.2 μm	Red	580/605 nm	10 mL
F8812	0.5 μm	Red	580/605 nm	10 mL
F8814	1.0 μm	青色	365/415 nm	10 mL
F8815	1.0 μm	青色	350/440 nm	10 mL
F8819	1.0 μm	ナイルレッド	535/575 nm	10 mL
F8821	1.0 μm	Red	580/605 nm	10 mL
F8824 または、製品番号F-8824	2.0 μm	青色	365/415 nm	2 mL
F8826	2.0 μm	Red	580/605 nm	2 mL

37 オプション

製品番号（カタログ番号） F8806

価格（JPY）

95,100

Each

お問い合わせください ›

直径（メートル法）:

0.2 μm

色:

クリムゾン

励起／発光:

625/645 nm

数量:

2 mL

フローサイトメトリー、顕微鏡、HTS、HCS、免疫測定法、当社の多様なFluoSpheresカルボン酸修飾ミクロスフェアを用いるその他のラボラトリー用途にも対応します。FluoSphereビーズは、粒子捕捉アプリケーションとして、タンパク質、核酸、生体分子の受動的吸着または能動的な共有結合に使用することができます。FluoSphereミクロスフェアには独自の蛍光色素が添加されており、もっとも明るいミクロスフェアとして使用できます。

当社のCarboxylate-Modified FluoSphere Microspheres（カルボン酸修飾FluoSphereミクロスフェア）は、ポリスチレンミクロスフェアから製造され、さまざまな独自の色素で充填されており、蛍光顕微鏡、フローサイトメトリー、HTS、HCS、細胞トレーシングなどのラボラトリー用のもっとも明るい蛍光を可視化します。特殊な染色法を使用することで、ビーズ表面ではなく各ポリスチレンミクロスフェア内に、すべての蛍光色素分子を収容できます。こうしたビーズ内での保護環境は、光退色などの有害な環境影響から色素を保護します。当社のカルボン酸修飾ミクロスフェアは、リガンドの共有結合性を実現するために、複数のカルボン酸を含んだ親水性ポリマーでコーティングされています。さまざまな研究用途や実験に使用できるように、各サイズの粒径をご用意しています。

For Research Use Only. Not for use in diagnostic procedures.

仕様

励起／発光625/645 nm

製品ラインFLUOSPHERES

数量2 mL

表面修飾カルボン酸塩

色クリムゾン

直径（メートル法）0.2 μm

使用対象（アプリケーション）蛍光顕微鏡法

材料ポリスチレン

製品タイプカルボン酸修飾ミクロスフェア

Unit SizeEach

組成および保存条件

冷蔵庫（2～8℃）に保存し、遮光。

よくあるご質問（FAQ）

I have some FluoSpheres polystyrene microspheres, with 20 nm diameter. They are aggregating a lot. What can I do about it?

The smaller the microspheres, the greater the propensity to aggregate. But the aggregation is not irreversible. Sonicate in a bath sonicator or vortex to disperse, just prior to use. You can also add a small concentration of Tween-20 or Triton X-100 (unless you are using them in a live-cell system).

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

I sonicated my 2.0 µm carboxylate-modified microspheres, as recommended, but saw foaming (bubbles) on top of the solution. Should I be concerned?

Use of a bath sonicator is recommended to help break up any aggregated microspheres. The foaming is from Tween-20, which is in the stock solution to help prevent aggregation. It is normal and expected to see bubbles from this. Do not use a probe sonicator, which would cause damage to the microspheres (as well as much more bubbling).

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

What is the warranty for FluoSpheres microspheres?

The warranty period for FluoSpheres microspheres is 1-year from the date of shipment.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

After washing and centrifugation, there was only a very small pellet left of my microsphere beads and the solution was transparent. Why is this?

Centrifugation is not an effective way to collect smaller microspheres; many particles remain in the solution even if you can visualize a small pellet. For beads less than 1 µm in diameter, we recommend washing by either:

Cross-flow filtration, as these particles have a very high compression modulus and can withstand high g-forces without risk of harm or dialysis with a 500 kDa MWCO
Note: Microspheres greater than 1 µm in diameter can be centrifuged at 1,300 rpm.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

I've had my microspheres for over a year, and I'm wondering if they're still good to use. What are some good ways to check their functionality?

Bacterial contamination is the most common cause of microspheres becoming unusable. Many of our particles are supplied with a low level of sodium azide to prevent bacterial contamination, but sometimes this can still occur. Bacterial contamination is best assessed by plating on appropriate growth medium and checking the plates after 72 hr.

Find additional tips, troubleshooting help, and resources within ourMicrospheres Support Center.

View all FluoSpheres™ Carboxylate-Modified Microspheres FAQs

引用および参考文献 (2)

引用および参考文献

Abstract

Cell lines as candidate reference materials for quality control of ERBB2 amplification and expression assays in breast cancer.

Authors:Xiao Y, Gao X, Maragh S, Telford WG, Tona A,

Journal:Clin Chem

PubMed ID:19443566

Human epidermal growth factor receptor 2 (HER2) is an important biomarker whose status plays a pivotal role in therapeutic decision-making for breast cancer patients and in determining their clinical outcomes. Ensuring the accuracy and reproducibility of HER2 assays by immunohistochemistry (IHC) and by fluorescence in situ hybridization (FISH) requires a ... More

Quantitation of HER2 and telomerase biomarkers in solid tumors with IgY antibodies and nanocrystal detection.

Authors:Xiao Y, Gao X, Gannot G, Emmert-Buck MR, Srivastava S, Wagner PD, Amos MD, Barker PE,

Journal:Int J Cancer

PubMed ID:18214859

In an effort to improve affinity biomarker validation in fixed patient tissue specimens, we have developed a novel quantum dot-based bioimaging system that utilizes chicken IgY antibody for high sensitivity and specificity relative quantitation of cancer proteins. Monospecific, polyclonal IgYs were generated against human HER2 and telomerase, and analytically validated ... More

FluoSpheres™ Carboxylate-Modified Microspheres

よくあるご質問（FAQ）

I have some FluoSpheres polystyrene microspheres, with 20 nm diameter. They are aggregating a lot. What can I do about it?

I sonicated my 2.0 &micro;m carboxylate-modified microspheres, as recommended, but saw foaming (bubbles) on top of the solution. Should I be concerned?

What is the warranty for FluoSpheres microspheres?

After washing and centrifugation, there was only a very small pellet left of my microsphere beads and the solution was transparent. Why is this?

I've had my microspheres for over a year, and I'm wondering if they're still good to use. What are some good ways to check their functionality?

引用および参考文献 (2)

I sonicated my 2.0 µm carboxylate-modified microspheres, as recommended, but saw foaming (bubbles) on top of the solution. Should I be concerned?