FluoSpheres™ Carboxylate-Modified Microspheres

Citations & References (2)

Invitrogen™

FluoSpheres™ Carboxylate-Modified Microspheres

Name: FluoSpheres™ Carboxylate-Modified Microspheres
SKU: F8814

各種の色および粒子サイズを揃えたカルボン酸修飾FluoSphereミクロスフェアを使用することで、もっとも明るい蛍光が得られます。

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製品番号（カタログ番号）	直径（メートル法）	色	励起／発光	数量
F8814	1.0 μm	青色	365/415 nm	10 mL
F8803	0.1 μm	黄-緑	505/515 nm	10 mL
F8816	1.0 μm	クリムゾン	625/645 nm	2 mL
F8823	1.0 μm	黄-緑	505/515 nm	10 mL
F8801	0.1 μm	Red	580/605 nm	10 mL
F8811	0.2 μm	黄緑色	505/515 nm	10 mL
F8807	0.2 μm	暗赤色	660/680 nm	2 mL
F10720	0.04 μm	黄～緑, オレンジ, 赤, ダークレッド	505/515、540/560、580/605、660/680 nm	1 mL（各）
F20881	0.2 μm	オレンジ	365/610 nm	2 mL
F8783 または、製品番号F-8783	0.02 μm	暗赤色	660/680 nm	2 mL
F8786	0.02 μm	Red	580/605 nm	10 mL
F8795 または、製品番号F-8795	0.04 μm	黄緑色	505/515 nm	1 mL
F8813	0.5 μm	黄緑色	505/515 nm	10 mL
F8820	1.0 μm	オレンジ	540/560 nm	10 mL
F8825	2.0 μm	ナイルレッド	535/575 nm	2 mL
F8827	2.0 μm	黄-緑	505/515 nm	2 mL
F8781 または、製品番号F-8781	0.02 μm	青色	365/415 nm	10 mL
F8782 または、製品番号F-8782	0.02 μm	クリムゾン	625/645 nm	2 mL
F8784	0.02 μm	ナイルレッド	535/575 nm	10 mL
F8787	0.02 μm	黄-緑	505/515 nm	10 mL
F8789 または、製品番号F-8789	0.04 μm	暗赤色	660/680 nm	1 mL
F8792 または、製品番号F-8792	0.04 μm	オレンジ	540/560 nm	1 mL
F8793	0.04 μm	Red	580/605 nm	1 mL
F8794	0.04 μm	赤色～オレンジ	565/580 nm	1 mL
F8797	0.1 μm	青色	350/440 nm	10 mL
F8799	0.1 μm	赤外線	715/755 nm	1 mL
F8800 または、製品番号F-8800	0.1 μm	オレンジ	540/560 nm	10 mL
F8805	0.2 μm	青色	365/415 nm	10 mL
F8806	0.2 μm	クリムゾン	625/645 nm	2 mL
F8809	0.2 μm	オレンジ	540/560 nm	10 mL
F8810	0.2 μm	Red	580/605 nm	10 mL
F8812	0.5 μm	Red	580/605 nm	10 mL
F8815	1.0 μm	青色	350/440 nm	10 mL
F8819	1.0 μm	ナイルレッド	535/575 nm	10 mL
F8821	1.0 μm	Red	580/605 nm	10 mL
F8824 または、製品番号F-8824	2.0 μm	青色	365/415 nm	2 mL
F8826	2.0 μm	Red	580/605 nm	2 mL

37 オプション

製品番号（カタログ番号） F8814

価格（JPY）

57,000

キャンペーン価格

Ends: 26-Dec-2025

95,100

割引額 38,100 (40%)

Each

お問い合わせください ›

直径（メートル法）:

1.0 μm

色:

青色

励起／発光:

365/415 nm

数量:

10 mL

フローサイトメトリー、顕微鏡、HTS、HCS、免疫測定法、当社の多様なFluoSpheresカルボン酸修飾ミクロスフェアを用いるその他のラボラトリー用途にも対応します。FluoSphereビーズは、粒子捕捉アプリケーションとして、タンパク質、核酸、生体分子の受動的吸着または能動的な共有結合に使用することができます。FluoSphereミクロスフェアには独自の蛍光色素が添加されており、もっとも明るいミクロスフェアとして使用できます。

当社のCarboxylate-Modified FluoSphere Microspheres（カルボン酸修飾FluoSphereミクロスフェア）は、ポリスチレンミクロスフェアから製造され、さまざまな独自の色素で充填されており、蛍光顕微鏡、フローサイトメトリー、HTS、HCS、細胞トレーシングなどのラボラトリー用のもっとも明るい蛍光を可視化します。特殊な染色法を使用することで、ビーズ表面ではなく各ポリスチレンミクロスフェア内に、すべての蛍光色素分子を収容できます。こうしたビーズ内での保護環境は、光退色などの有害な環境影響から色素を保護します。当社のカルボン酸修飾ミクロスフェアは、リガンドの共有結合性を実現するために、複数のカルボン酸を含んだ親水性ポリマーでコーティングされています。さまざまな研究用途や実験に使用できるように、各サイズの粒径をご用意しています。

For Research Use Only. Not for use in diagnostic procedures.

仕様

励起／発光365/415 nm

製品ラインFLUOSPHERES

数量10 mL

表面修飾カルボン酸塩

色青色

直径（メートル法）1.0 μm

使用対象（アプリケーション）蛍光顕微鏡法

材料ポリスチレン

製品タイプカルボン酸修飾ミクロスフェア

Unit SizeEach

組成および保存条件

冷蔵庫（2～8℃）に保存し、遮光。

よくあるご質問（FAQ）

I read that carboxylate-modified latex (CML) beads have a "fluffy coat" of carboxyl groups on their surface. What is meant by this?

The CML beads have a high density of carboxyl groups at the surface. The surface layer is quite hydrophilic and at the appropriate pH (pH>5), are charged; due to electrostatic repulsion, this type of surface is 3-dimensional and may be considered analogous to the fuzz on a tennis ball.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

I have some FluoSpheres polystyrene microspheres, with 20 nm diameter. They are aggregating a lot. What can I do about it?

The smaller the microspheres, the greater the propensity to aggregate. But the aggregation is not irreversible. Sonicate in a bath sonicator or vortex to disperse, just prior to use. You can also add a small concentration of Tween-20 or Triton X-100 (unless you are using them in a live-cell system).

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

I sonicated my 2.0 µm carboxylate-modified microspheres, as recommended, but saw foaming (bubbles) on top of the solution. Should I be concerned?

Use of a bath sonicator is recommended to help break up any aggregated microspheres. The foaming is from Tween-20, which is in the stock solution to help prevent aggregation. It is normal and expected to see bubbles from this. Do not use a probe sonicator, which would cause damage to the microspheres (as well as much more bubbling).

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

What is the warranty for FluoSpheres microspheres?

The warranty period for FluoSpheres microspheres is 1-year from the date of shipment.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

After washing and centrifugation, there was only a very small pellet left of my microsphere beads and the solution was transparent. Why is this?

Centrifugation is not an effective way to collect smaller microspheres; many particles remain in the solution even if you can visualize a small pellet. For beads less than 1 µm in diameter, we recommend washing by either:

Cross-flow filtration, as these particles have a very high compression modulus and can withstand high g-forces without risk of harm or dialysis with a 500 kDa MWCO
Note: Microspheres greater than 1 µm in diameter can be centrifuged at 1,300 rpm.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

View all FluoSpheres™ Carboxylate-Modified Microspheres FAQs

引用および参考文献 (2)

引用および参考文献

Abstract

Counting dendritic spines in brain tissue slices by image correlation spectroscopy analysis.

Authors:Wiseman PW, Capani F, Squier JA, Martone ME

Journal:J Microsc

PubMed ID:11879432

Growth of new micrometre sized projections called dendritic spines in neurones has been linked to the encoding of long-term memories in vertebrates. Numerous studies have been carried out at both the light and electron microscopy level to quantify dendritic spine densities in brain tissue in laboratory animals. Currently, such efforts ... More

Angiogenesis monitored by perfusion with a space-filling microbead suspension.

Authors:Springer ML, Ip TK, Blau HM

Journal:Mol Ther

PubMed ID:10933915

Numerous laboratories are focusing efforts on delivering gene products to induce or prevent the development of new blood vessels in adults, with the hope of rescuing ischemic tissues, circumventing cardiac bypass surgery, or inhibiting tumor growth. Current approaches to the assessment of vascular continuity involve the introduction of either dyes ... More

FluoSpheres™ Carboxylate-Modified Microspheres

よくあるご質問（FAQ）

I read that carboxylate-modified latex (CML) beads have a "fluffy coat" of carboxyl groups on their surface. What is meant by this?

I have some FluoSpheres polystyrene microspheres, with 20 nm diameter. They are aggregating a lot. What can I do about it?

I sonicated my 2.0 &micro;m carboxylate-modified microspheres, as recommended, but saw foaming (bubbles) on top of the solution. Should I be concerned?

What is the warranty for FluoSpheres microspheres?

After washing and centrifugation, there was only a very small pellet left of my microsphere beads and the solution was transparent. Why is this?

引用および参考文献 (2)

I sonicated my 2.0 µm carboxylate-modified microspheres, as recommended, but saw foaming (bubbles) on top of the solution. Should I be concerned?