Guanosine 5'-Triphosphate, BODIPY™ FL 2'-(or-3')-O-(N-(2-Aminoethyl) Urethane), Trisodium Salt (BODIPY™ FL GTP)

Guanosine 5'-Triphosphate, BODIPY™ FL 2'-(or-3')-<i>O</i>-(<i>N</i>-(2-Aminoethyl) Urethane), Trisodium Salt (BODIPY™ FL GTP)

Citations & References (8)

Invitrogen™

Guanosine 5'-Triphosphate, BODIPY™ FL 2'-(or-3')-O-(N-(2-Aminoethyl) Urethane), Trisodium Salt (BODIPY™ FL GTP)

BODIPY FLアデノシン5'-三リン酸（A-12410）およびBODIPY FLグアノシン5'-三リン酸は、リンカーを介してリボース環の2'位または3'位に結合されたBODIPY FLフルオロフォアで構成されます。これらのヌクレオチド類似体の分光特性から、フルオロフォアとプリン塩基の相互作用が明らかになります。BODIPY詳細を見る

製品番号（カタログ番号）	数量
G12411	100 µL

製品番号（カタログ番号） G12411

価格（JPY）

68,200

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100 µL

BODIPY FLアデノシン5'-三リン酸（A-12410）およびBODIPY FLグアノシン5'-三リン酸は、リンカーを介してリボース環の2'位または3'位に結合されたBODIPY FLフルオロフォアで構成されます。これらのヌクレオチド類似体の分光特性から、フルオロフォアとプリン塩基の相互作用が明らかになります。BODIPY FL GTPの蛍光量子収率は、BODIPY FL ATPの10分の1にすぎません。ただしこの収率は、BODIPY FL GTPをGTP結合タンパク質に結合することで向上させることができます。

研究用にのみ使用できます。診断用には使用いただけません。

標識または色素BODIPY™ FL

製品タイプグアノシン5'-三リン酸塩

数量100 µL

出荷条件湿氷

濃度5 mM

製品ラインBODIPY

Unit SizeEach

組成および保存条件

フリーザー（-5℃～-30℃）に保存し、遮光してください。

引用および参考文献 (8)

引用および参考文献

Abstract

Capillary electrophoresis assay for G protein-coupled receptor-mediated GTPase activity.

Authors:Jameson EE, Pei J, Wade SM, Neubig RR, Milligan G, Kennedy RT

Journal:Anal Chem

PubMed ID:17263349

'We describe a capillary electrophoresis (CE) assay to detect G protein-coupled receptor (GPCR)-stimulated G protein GTPase activity in cell membranes expressing alpha2A adrenoreceptor-Galphao1 wild-type (wt) or C351I mutant fusion proteins using a fluorescent, hydrolyzable GTP analogue. As no change in total fluorescence is observed by conversion of substrate to product, ... More

Automated single-cell electroporation.

Authors:Bae C, Butler PJ

Journal:Biotechniques

PubMed ID:17068953

Identification of a small GTPase inhibitor using a high-throughput flow cytometry bead-based multiplex assay.

Authors:Surviladze Z, Waller A, Wu Y, Romero E, Edwards BS, Wandinger-Ness A, Sklar LA,

Journal:J Biomol Screen

PubMed ID:20008126

Small GTPases are key regulators of cellular activity and represent novel targets for the treatment of human diseases using small-molecule inhibitors. The authors describe a multiplex, flow cytometry bead-based assay for the identification and characterization of inhibitors or activators of small GTPases. Six different glutathione-S-transferase (GST)-tagged small GTPases were bound ... More

A direct fluorescence-based assay for RGS domain GTPase accelerating activity.

Authors:Willard FS, Kimple AJ, Johnston CA, Siderovski DP

Journal:Anal Biochem

PubMed ID:15840508

Diverse extracellular signals regulate seven transmembrane-spanning receptors to modulate cellular physiology. These receptors signal primarily through activation of heterotrimeric guanine nucleotide binding proteins (G proteins). A major determinant of heterotrimeric G protein signaling in vivo and in vitro is the intrinsic GTPase activity of the Galpha subunit. RGS (regulator of ... More

Real-time detection of basal and stimulated G protein GTPase activity using fluorescent GTP analogues.

Authors:Jameson EE, Roof RA, Whorton MR, Mosberg HI, Sunahara RK, Neubig RR, Kennedy RT

Journal:J Biol Chem

PubMed ID:15613467

Hydrolysis of fluorescent GTP analogues BODIPY FL guanosine 5 '-O-(thiotriphosphate) (BGTPgammaS) and BODIPY FL GTP (BGTP) by Galpha(i1) and Galpha was characterized using on-line capillary electrophoresis (o) laser-induced fluorescence assays in order that changes in sub-strate, substrate-enzyme complex, and product could be monitored separately. Apparent k values (V /[E]) (max ... More

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