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Citations & References (28)
Invitrogen™
Guanosine 5'-O-(3-Thiotriphosphate), BODIPY™ FL Thioester, Sodium Salt (BODIPY™ FL GTP-γ-S, Thioester)
BODIPY FL GTP-γ-Sは、GTP結合タンパク質に結合します。この蛍光ヌクレオチドは、GTP-γ-Sのγ-チオールを介してBODIPY FLフルオロフォアを結合することで作製されています。BODIPY FL GTP-γ-Sの蛍光は詳細を見る
| 製品番号（カタログ番号） | 数量 |
|---|---|
| G22183 または、製品番号G-22183 | 50 µL |
製品番号（カタログ番号） G22183
または、製品番号G-22183
価格（JPY）お問い合わせください ›
140,400
Each
数量:
50 µL
BODIPY FL GTP-γ-Sは、GTP結合タンパク質に結合します。この蛍光ヌクレオチドは、GTP-γ-Sのγ-チオールを介してBODIPY FLフルオロフォアを結合することで作製されています。BODIPY FL GTP-γ-Sの蛍光は、遊離色素の蛍光と比較して約90%消光されますが、Gタンパク質との結合により回復します。BODIPY FL GTP-γ-Sチオエーテルは、結合研究に使用できる可能性があるだけでなく、FHIT（ヌクレオチド結合タンパク質のヒスチジントライアドスーパーファミリーのメンバー）に重要な新しい基質です。
研究用にのみ使用できます。診断用には使用いただけません。
仕様
標識法化学標識、直接標識
標識または色素BODIPY™ FL
製品タイプグアノシン5'-O
数量50 µL
出荷条件湿氷
濃度5 mM
製品ラインBODIPY
Unit SizeEach
組成および保存条件
フリーザー（-5℃～-30℃）に保存し、遮光してください。
引用および参考文献 (28)
引用および参考文献
Abstract
Detection of G proteins by affinity probe capillary electrophoresis using a fluorescently labeled GTP analogue.
Journal:Anal Chem
PubMed ID:14632149
'An affinity probe capillary electrophoresis (APCE) assay for guanine-nucleotide-binding proteins (G proteins) was developed using BODIPY FL GTPgammaS (BGTPgammaS), a fluorescently labeled GTP analogue, as the affinity probe. In the assay, BGTPgammaS was incubated with samples containing G proteins and the resulting mixtures of BGTPgammaS-G protein complexes and free BGTPgammaS
Designed FHIT alleles establish that Fhit-induced apoptosis in cancer cells is limited by substrate binding.
Journal:Proc Natl Acad Sci U S A
PubMed ID:12574506
'The FHIT gene is inactivated early in the development of many human tumors, and Fhit-deficient mice have increased cancer incidence. Viral reexpression of Fhit kills Fhit-deficient cells by induction of apoptosis. Fhit, a member of branch 2 of the histidine-triad superfamily of nucleoside monophosphate hydrolases and transferases, is a diadenosine
Fhit-nucleotide specificity probed with novel fluorescent and fluorogenic substrates.
Journal:J Biol Chem
PubMed ID:10671479
'Fhit, a member of the histidine triad superfamily of nucleotide-binding proteins, binds and cleaves diadenosine polyphosphates and functions as a tumor suppressor in human epithelial cancers. Function of Fhit in tumor suppression does not require diadenosine polyphosphate cleavage but correlates with the ability to form substrate complexes. As diadenosine polyphosphates
Mechanism of regulation of prokaryotic tubulin-like GTPase FtsZ by membrane protein EzrA.
Journal:J Biol Chem
PubMed ID:17043359
'At initiation of cell division, FtsZ, a tubulin-like GTPase, assembles into a so-called Z-ring structure at the site of division. The formation of Z ring is negatively regulated by EzrA, which ensures only one ring at the midcell per cell cycle. The mechanism leading to the negative regulation of Z-ring
Use of a native affinity ligand for the detection of G proteins by capillary isoelectric focusing with laser-induced fluorescence detection.
Journal:Electrophoresis
PubMed ID:15274014
'Affinity probe capillary isoelectric focusing (CIEF) with laser-induced fluorescence was explored for detection of Ras-like G proteins. In the assay, a fluorescent BODIPY FL GTP analogue (BGTPgammaS) and G protein were incubated resulting in formation of BGTPgammaS-G protein complex. Excess BGTPgammaS was separated from BGTPgammaS-G protein complex by CIEF using