HCS Mitochondrial Health Kit
HCS Mitochondrial Health Kit
Invitrogen™

HCS Mitochondrial Health Kit

HCS Mitochondrial Health Kitは、2つの重要な細胞健康パラメータであるミトコンドリア毒性と細胞毒性を同時に定量的に測定します。MitoHealth染色剤はミトコンドリア膜電位の変化を検出し、これに基づき試薬が活性ミトコンドリアに蓄積します。このキットには、細胞毒性を検出するためのImage-iT™ DEAD™詳細を見る
製品番号(カタログ番号)数量
H102951 kit
製品番号(カタログ番号) H10295
価格(JPY)
77,100
Each
お問い合わせください ›
数量:
1 kit
HCS Mitochondrial Health Kitは、2つの重要な細胞健康パラメータであるミトコンドリア毒性と細胞毒性を同時に定量的に測定します。MitoHealth染色剤はミトコンドリア膜電位の変化を検出し、これに基づき試薬が活性ミトコンドリアに蓄積します。このキットには、細胞毒性を検出するためのImage-iT™ DEAD™ Greenが含まれます。この色素は、健康な細胞に対しては細胞膜非透過性ですが、原形質膜が損傷した細胞には浸透し、染色を行います。どちらのインジケータも固定および透過処理に適しているため、必要な他のバイオマーカーとの多重化が可能です。キットには、核を区分するためのツールである青色蛍光Hoechst 33342も含まれます。この色素は生細胞、死細胞の両方のDNAを染色します。
研究用にのみ使用できます。診断用には使用いただけません。
仕様
検出法蛍光
染色剤タイプImage-iT™ DEAD Green細胞生存率検出用染色剤、MitoHealth染色剤、Hoechst 33342
フォーマット96-ウェルプレート
数量1 kit
出荷条件室温
オレンジ、青、緑
使用対象 (装置)蛍光顕微鏡, ハイコンテント装置
製品ラインMolecular Probes
製品タイプMitochondrial Health Kit
Unit SizeEach
組成および保存条件
このキットには(96ウェルプレートフォーマットで2枚のプレートを実行するのに十分な試薬が含まれています。
  • ≦-20℃で保存し、乾燥させて遮光してください。
  • よくあるご質問(FAQ)

    What is the fluorescence excitation/emission maxima of the stains in the HCS mitochondrial health kit?

    The fluorescence excitation/emission maxima are as follows: Hoechst 33342 - 350/461 nm bound to DNA; Image-iT DEAD Green viability stain - 488/515 nm; MitoHealth stain - 550/580 nm.

    Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

    引用および参考文献 (14)

    引用および参考文献
    Abstract
    Nullomer derived anticancer peptides (NulloPs): differential lethal effects on normal and cancer cells in vitro.
    Authors:Alileche A, Goswami J, Bourland W, Davis M, Hampikian G,
    Journal:Peptides
    PubMed ID:23000474
    'We demonstrate the first use of the nullomer (absent sequences) approach to drug discovery and development. Nullomers are the shortest absent sequences determined in a species, or group of species. By identifying the shortest absent peptide sequences from the NCBI databases, we screened several potential anti-cancer peptides. In order to ... More
    High Content Analysis technology for evaluating the joint toxicity of sunset yellow and sodium sulfite in vitro.
    Authors:Qu D, Gu Y, Feng L, Han J
    Journal:Food Chem
    PubMed ID:28530558
    'Foods contain various additives that affect our daily lives. At present, food additive safety evaluation standards are based on the toxicity of single additives, but food additives are often used in combination and may have additive, synergistic or antagonistic actions. The current study investigated the toxicity of food additives and ... More
    Vitamin D regulates prostate cell metabolism via genomic and non-genomic mitochondrial redox-dependent mechanisms.
    Authors:Blajszczak CC, Nonn L
    Journal:J Steroid Biochem Mol Biol
    PubMed ID:31574299
    'Vitamin D deficiency has been associated with increased risk for aggressive prostate cancer (PCa). Prostate epithelium has a unique metabolism compared to other tissues. Normal prostate exhibits low levels of mitochondrial respiration and there is a metabolic switch to increased oxidative phosphorylation in PCa. 25-hydroxyvitamin D (25(OH)D) is the major ... More
    Handling and Assessment of Human Primary Prostate Organoid Culture.
    Authors:McCray T, Richards Z, Marsili J, Prins GS, Nonn L,
    Journal:J Vis Exp
    PubMed ID:30735176
    'This paper describes a detailed protocol for three-dimensional (3D) culturing, handling, and evaluation of human primary prostate organoids. The process involves seeding of epithelial cells sparsely in a 3D matrix gel on a 96-well microplate with media changes to cultivate expansion into organoids. Morphology is then assessed by whole-well capturing ... More
    A high throughput imaging database of toxicological effects of nanomaterials tested on HepaRG cells.
    Authors:Joossens E, Macko P, Palosaari T, Gerloff K, Ojea-Jiménez I, Gilliland D, Novak J, Fortaner Torrent S, Gineste JM, Römer I, Briffa SM, Valsami-Jones E, Lynch I, Whelan M,
    Journal:Sci Data
    PubMed ID:31048742
    'The large amount of existing nanomaterials demands rapid and reliable methods for testing their potential toxicological effect on human health, preferably by means of relevant in vitro techniques in order to reduce testing on animals. Combining high throughput workflows with automated high content imaging techniques allows deriving much more information ... More