Hypoxia Green Reagent for Flow Cytometry

フローサイトメトリー用のHypoxia Green Reagentは、生細胞の低酸素濃度を検出するために使用される抗体非依存試薬です。膜透過性プローブは、酸素濃度が低下するとローダミンを放出し、蛍光反応をもたらします。低酸素症は、心血管疾患や腫瘍による免疫抑制など、多くの疾患の特徴であり詳細を見る

製品番号（カタログ番号）	数量
H20035	2 x 50 assays

製品番号（カタログ番号） H20035

価格（JPY）

83,600

お問い合わせください ›

2 x 50 assays

フローサイトメトリー用のHypoxia Green Reagentは、生細胞の低酸素濃度を検出するために使用される抗体非依存試薬です。膜透過性プローブは、酸素濃度が低下するとローダミンを放出し、蛍光反応をもたらします。低酸素症は、心血管疾患や腫瘍による免疫抑制など、多くの疾患の特徴であり、腫瘍の生存と増殖に不可欠です。

•高感度で固定されたエンドポイントアッセイ
• 生細胞における酸素の減少を検出します
• 蛍光性非抗体ベースのプローブ

検出法蛍光

フォーマットバイアル

数量2 x 50 assays

出荷条件室温

Emission488

使用対象 (装置)フローサイトメーター

製品タイプ試薬

Unit SizeEach

組成および保存条件

50回の試験ごとに2本のバイアル

–5℃～–30℃で保存してください。

よくあるご質問（FAQ）

Can the Hypoxia Green Reagent for Flow Cytometry be used in cells expressing fluorescent proteins?

Yes, as long as the fluorescent protein does not emit in the green range (˜510–535 nm). For cells that do not express a fluorescent protein, we recommend viewing unstained cells under the FITC channel to examine autofluorescence. For cells expressing a fluorescent protein, we recommend analyzing unstained cell samples to determine the extent the fluorescent protein emission may overlap in the FITC channel.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Our Hypoxia Green Reagent for Flow Cytometry is already highly fluorescent before using the product. What could have caused this?

This could be due to storage of the product under anaerobic or low oxygen conditions. The Hypoxia Green Reagent for Flow Cytometry increases in fluorescence upon exposure to low oxygen environments, and this change is not reversible. For some ROS indicators, we recommend storing the reagent under dry nitrogen or argon to prevent oxidation during storage. However, this is not appropriate for the Hypoxia Green Reagent for Flow Cytometry.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

What is different between the use of the Hypoxia Green Reagent for Flow Cytometry (Cat No. H20035) versus imaging using the Image-iT Green Hypoxia Reagent (Cat Nos. I14833, I14834)?

The difference between these products is in the final working concentration of the reagent and incubation time. For flow cytometry applications, we recommend using a final concentration in the range of 0.5 to 1 µM with an incubation time ranging from 2 to 3 hrs. For imaging, we recommend a final concentration in the range of 1 to 10 µM with an incubation time ranging from 30 mins to 1 hr. For both applications, one should optimize the final working concentration and incubation time.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Is Hypoxia Green Reagent for Flow Cytometry (Cat No. H20035) the same reagent as the Image-iT Green Hypoxia Reagent (Cat Nos. I14833, I14834)?

The Hypoxia Green Reagent for Flow Cytometry and the Image-iT Green Hypoxia Reagent are the same reagent, formulated differently for flow cytometry and imaging use, respectively.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

引用および参考文献 (2)

引用および参考文献

Abstract

Catalase-loaded cisplatin-prodrug-constructed liposomes to overcome tumor hypoxia for enhanced chemo-radiotherapy of cancer.

Authors:Zhang R, Song X, Liang C, Yi X, Song G, Chao Y, Yang Y, Yang K, Feng L, Liu Z

Journal:Biomaterials

PubMed ID:28550753

Aiming at improved therapeutic efficacies, the combination of chemotherapy and radiotherapy (chemo-radiotherapy) has been widely studied and applied in clinic. However, the hostile characteristics of tumor microenvironment such as hypoxia often limit the efficacies in both types of cancer therapies. Herein, catalase (CAT), an antioxidant enzyme, is encapsulated inside liposomes ... More

Tumor-targeted nanoplatform for in situ oxygenation-boosted immunogenic phototherapy of colorectal cancer.

Authors:He H, Liu L, Liang R, Zhou H, Pan H, Zhang S, Cai L

Journal:Acta Biomater

PubMed ID:31945508

Advanced colorectal cancer has a high mortality rate since conventional treatments have limited therapeutic effects and poor prognosis with high risks of metastasis and recurrence. Photodynamic therapy (PDT) is a promising treatment modality for the eradication of colorectal cancer, but its curative efficacy is severely affected by tumor hypoxia. Herein, ... More