Hexidium Iodide
Hexidium Iodide
Invitrogen™

Hexidium Iodide

核酸染色であるヘキシジウムヨウ化物は、哺乳類細胞に浸透し、グラム陰性細菌が存在する場合にほぼすべてのグラム陽性細菌を選択的に染色します。ヘキシジウムヨウ化物は、結合DNAにおいて最大約518/600 nmの励起/最大発光を示します。一般的に、真核細胞の細胞質と核の両方に、ヘキシジウムヨウ化物による染色が見られます詳細を見る
製品番号(カタログ番号)数量
H75935 mg
製品番号(カタログ番号) H7593
価格(JPY)
42,500
온라인 행사
Ends: 27-Mar-2026
70,900
割引額 28,400 (40%)
Each
お問い合わせください ›
数量:
5 mg
核酸染色であるヘキシジウムヨウ化物は、哺乳類細胞に浸透し、グラム陰性細菌が存在する場合にほぼすべてのグラム陽性細菌を選択的に染色します。ヘキシジウムヨウ化物は、結合DNAにおいて最大約518/600 nmの励起/最大発光を示します。一般的に、真核細胞の細胞質と核の両方に、ヘキシジウムヨウ化物による染色が見られます。
研究用にのみ使用できます。診断用には使用いただけません。
仕様
オレンジ
検出法蛍光
染色剤タイプ細胞透過性
励起波長域518⁄600
使用対象 (装置)蛍光顕微鏡
数量5 mg
出荷条件室温
標識タイプFluorescent Dye
製品タイプ核酸染色
SubCellular Localization核, 細胞質 & サイトゾル, Cytosol, Nucleus
Unit SizeEach
組成および保存条件
室温で保存し、光から保護します。

引用および参考文献 (10)

引用および参考文献
Abstract
A flow-cytometric gram-staining technique for milk-associated bacteria.
Authors:Holm C, Jespersen L
Journal:Appl Environ Microbiol
PubMed ID:12732558
'A Gram-staining technique combining staining with two fluorescent stains, Oregon Green-conjugated wheat germ agglutinin (WGA) and hexidium iodide (HI) followed by flow-cytometric detection is described. WGA stains gram-positive bacteria while HI binds to the DNA of all bacteria after permeabilization by EDTA and incubation at 50 degrees C for 15 ... More
A fluorescent Gram stain for flow cytometry and epifluorescence microscopy.
Authors:Mason DJ, Shanmuganathan S, Mortimer FC, Gant VA
Journal:Appl Environ Microbiol
PubMed ID:9647848
'The fluorescent nucleic acid binding dyes hexidium iodide (HI) and SYTO 13 were used in combination as a Gram stain for unfixed organisms in suspension. HI penetrated gram-positive but not gram-negative organisms, whereas SYTO 13 penetrated both. When the dyes were used together, gram-negative organisms were rendered green fluorescent by ... More
Metabolic activity in filamentous fungi can be analysed by flow cytometry.
Authors:Bradner JR, Nevalainen KM
Journal:J Microbiol Methods
PubMed ID:12782375
The use of flow cytometry in combination with fluorescent dyes as a technique to rapidly differentiate and enumerate bacterial and yeast cells is well established. We have shown that through the judicial choice of stains, the nondestructive screening and sorting of fungal material is possible. The early stages of growth, ... More
Increased sensitivity of bacterial detection in cerebrospinal fluid by fluorescent staining on low-fluorescence membrane filters.
Authors:Durtschi JD, Erali M, Bromley LK, Herrmann MG, Petti CA, Smith RE, Voelkerding KV
Journal:J Med Microbiol
PubMed ID:16091435
A membrane-filter-based, fluorescent Gram stain method for bacterial detection in cerebrospinal fluid samples was developed and evaluated as a rapid, sensitive alternative to standard Gram stain protocols. A recently developed, modified version of the aluminium oxide membrane Anopore with low-fluorescence optical properties showed superior performance in this application. Other aspects ... More
Nucleic acid stains as indicators of Cryptosporidium parvum oocyst viability.
Authors:Belosevic M, Guy RA, Taghi-Kilani R, Neumann NF, Gyürék LL, Liyanage LR, Millard PJ, Finch GR
Journal:Int J Parasitol
PubMed ID:9279581
We developed nucleic acid dye staining methodology for untreated, heat-treated and chemically inactivated C. parvum oocysts. The nucleic acid staining was compared to in vitro excystation and animal infectivity using split samples of oocysts. Among the nucleic acid stains tested, SYTO-9, hexidium and SYTO-59 stained the oocysts consistently, and the ... More