Image-IT™ DEAD Green™ Viability Stain
Image-IT™ DEAD Green™ Viability Stain
Citations & References (6)
Invitrogen™

Image-IT™ DEAD Green™ Viability Stain

Image-iT™ DEAD™ Green生存染色剤は、固定および透過処理にも対応する、迅速かつ高感度で使いやすい生存インジケーターであり、他の細胞毒性バイオマーカーとの多重化が可能です。Image-iT™ DEAD™ Greenは、健康な細胞に対しては細胞膜非透過性ですが詳細を見る
テクニカルサポートオーダーサポート
製品番号（カタログ番号）数量
I102911 Vial
製品番号（カタログ番号） I10291
価格（JPY）
69,200
Each
お問い合わせください ›
数量:
1 Vial
Image-iT™ DEAD™ Green生存染色剤は、固定および透過処理にも対応する、迅速かつ高感度で使いやすい生存インジケーターであり、他の細胞毒性バイオマーカーとの多重化が可能です。Image-iT™ DEAD™ Greenは、健康な細胞に対しては細胞膜非透過性ですが、原形質膜が損傷した細胞には浸透し、染色を行います。
研究用にのみ使用できます。診断用には使用いただけません。
仕様
濃度1 mM
検出法蛍光
形状液体
数量1 Vial
出荷条件湿氷
細胞内局在核
色Green
Emission可視
使用対象（アプリケーション）生存率アッセイ
使用対象 (装置)蛍光顕微鏡
製品ラインImage-iT、Molecular Probes
製品タイプ生存染色
Unit SizeEach
組成および保存条件
この染色剤には、96ウェルプレート仕様、およびウェル容量50 μLで10プレート分のアッセイに十分な材料が含まれています。
  • 遮光し、≤ −20°Cの乾燥した場所に保存。

    • よくあるご質問（FAQ）

    Do you offer a cell viability stain compatible with fixation?

    Our Image-IT DEAD Green Viability Stain (Cat. No. I10291) is amenable to fixation and permeabilization after the application of the stain. It is important to use fixatives free of alcohol or other organic solvents.

    Find additional tips, troubleshooting help, and resources within our Cell Viability, Proliferation, Cryopreservation, and Apoptosis Support Center.

    Which cell viability kits are compatible with fixation?

    The LIVE/DEAD Fixable kits for flow cytometry analysis are compatible with fixation. These kits use amine-reactive cell-impermeant dyes that stain the cell surface of live cells and also the cytosol of dead cells-live cells are dim and dead cells are bright. Since the dye is covalently bound to the cells, it will be retained after fixation. Unfortunately, this method does not work well for imaging-based assays, as all cells are stained and it is difficult to distinguish bright dead cells from dim live cells with a microscope. Ethidium monoazide (EMA; Cat No. E1374) is a cell impermeant nucleic acid stain that can be applied to live cultures and stains only dead cells. After incubation and washing away unbound dye, the cells can be exposed to light to photoactivate EMA to crosslink to dead cell DNA. After crosslinking to dead cell DNA, the samples may be fixed and permeabilized. Image-IT DEAD Green Viability Stain (Cat. No. I10291) for imaging and high-content screening (HCS) analysis is a live-cell impermeant DNA binding dye that is compatible with fixation and permeabilization with good retention up to 48 hours. We also have a LIVE/DEAD Reduced Biohazard Cell Viability Kit (Cat. No. L7013) for imaging and flow analysis that contains two DNA binding dyes, SYTO 10 and Dead Red, that are sufficiently retained to be analyzed soon after 4% glutaraldehyde fixation.
    Note: In general, DNA-binding dyes and calcein AM are not compatible with fixation, as these dyes are not covalently bound to components of the cell and will thus slowly diffuse out of cells after fixation, gradually staining all cells as dead.

    Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

    引用および参考文献 (6)

    引用および参考文献
    Abstract
    Global metabolic profiling of infection by an oncogenic virus: KSHV induces and requires lipogenesis for survival of latent infection.
    Authors:Delgado T, Sanchez EL, Camarda R, Lagunoff M,
    Journal:PLoS Pathog
    PubMed ID:22916018
    Like cancer cells, virally infected cells have dramatically altered metabolic requirements. We analyzed global metabolic changes induced by latent infection with an oncogenic virus, Kaposi's Sarcoma-associated herpesvirus (KSHV). KSHV is the etiologic agent of Kaposi's Sarcoma (KS), the most common tumor of AIDS patients. Approximately one-third of the nearly 200 ... More
    Antibody-targeted nanovectors for the treatment of brain cancers.
    Authors:Sharpe MA, Marcano DC, Berlin JM, Widmayer MA, Baskin DS, Tour JM,
    Journal:ACS Nano
    PubMed ID:22390360
    Introduced here is the hydrophilic carbon clusters (HCCs) antibody drug enhancement system (HADES), a methodology for cell-specific drug delivery. Antigen-targeted, drug-delivering nanovectors are manufactured by combining specific antibodies with drug-loaded poly(ethylene glycol)-HCCs (PEG-HCCs). We show that HADES is highly modular, as both the drug and antibody component can be varied ... More
    Analysis of cell cycle and replication of mouse macrophages after in vivo and in vitro Cryptococcus neoformans infection using laser scanning cytometry.
    Authors:Coelho C, Tesfa L, Zhang J, Rivera J, Gonçalves T, Casadevall A,
    Journal:Infect Immun
    PubMed ID:22252872
    We investigated the outcome of the interaction of Cryptococcus neoformans with murine macrophages using laser scanning cytometry (LSC). Previous results in our lab had shown that phagocytosis of C. neoformans promoted cell cycle progression. LSC allowed us to simultaneously measure the phagocytic index, macrophage DNA content, and 5-ethynyl-2'-deoxyuridine (EdU) incorporation ... More
    Induction of the Warburg effect by Kaposi's sarcoma herpesvirus is required for the maintenance of latently infected endothelial cells.
    Authors:Delgado T, Carroll PA, Punjabi AS, Margineantu D, Hockenbery DM, Lagunoff M,
    Journal:Proc Natl Acad Sci U S A
    PubMed ID:20498071
    Kaposi's sarcoma (KS) is the most commonly reported tumor in parts of Africa and is the most common tumor of AIDS patients world-wide. KS-associated herpesvirus (KSHV) is the etiologic agent of KS. Although KS tumors contain many cell types, the predominant cell is the spindle cell, a cell of endothelial ... More
    Miniaturized modular-array fluorescence microscopy.
    Authors:
    Journal:Biomed Opt Express
    PubMed ID:33408992
    View all Image-IT™ DEAD Green™ Viability Stain citations