Image-iT™ Green Hypoxia Reagent
Image-iT™ Green Hypoxia Reagent
Invitrogen™

Image-iT™ Green Hypoxia Reagent

Image-iT Green Hypoxia Reagentは、生細胞中の低酸素状態を測定するための、固定可能な新しい蛍光性化合物です。生細胞が正常な酸素濃度環境にある場合は無蛍光で、酸素濃度が低下すると蛍光を発します。Image-iT Green詳細を見る
製品番号(カタログ番号)数量
I148341バイアル
I148335バイアル
製品番号(カタログ番号) I14834
価格(JPY)
27,900
Each
お問い合わせください ›
数量:
1バイアル
Image-iT Green Hypoxia Reagentは、生細胞中の低酸素状態を測定するための、固定可能な新しい蛍光性化合物です。生細胞が正常な酸素濃度環境にある場合は無蛍光で、酸素濃度が低下すると蛍光を発します。Image-iT Green Hypoxia Reagentは、細胞/組織が正常な酸素濃度に戻っても蛍光は持続するため、蛍光シグナルの損失を最小限に抑えながら細胞/組織を固定できます。

Image-iT Green Hypoxia Reagentの特長:
•低酸素環境で蛍光を発することにより、生細胞中の低酸素状態を示唆
• 固定可能—細胞/組織が正常な酸素濃度に戻っても蛍光シグナルは持続
• 使いやすい—細胞培養培地に添加して画像を取得するだけ

Image-iT Green Hypoxia Reagentは生細胞透過性のある蛍光性化合物で、低酸素濃度環境で蛍光を発します。これらの特性により、この試薬は低酸素状態の細胞や組織を検出するのに非常に有用なツールです。Image-iT Green Hypoxia Reagentは非常に高感度な酸素検出試薬です。Image-iT Green Hypoxia Reagentは、非常に低い酸素濃度にのみ反応するピモニダゾール付加物とは異なり、周囲の酸素濃度が5%未満になると蛍光し始めます。このような環境に迅速に反応し、酸素濃度が正常に戻っても蛍光は持続します。これらの特性により、Image-iT Green Hypoxia Reagentは、腫瘍、3D培養、スフェロイド、ニューロンなどの低酸素状態を検出するのに最適なツールです。

研究用途にのみご使用ください。診断目的には使用できません。
仕様
数量1バイアル
試薬タイプ低酸素試薬
出荷条件氷水またはドライアイスでの出荷が承認されています
製品ラインImage-iT
製品タイプ試薬
Unit SizeEach
組成および保存条件
-5~-30℃で保存。遮光。
  • 受け取り後6カ月間は安定しています。
  • よくあるご質問(FAQ)

    Can Image-it Green Hypoxia Reagent be used in cells expressing fluorescent proteins?

    Yes, as long as the fluorescent protein does not emit in the green range (˜510–535 nm). For cells that do not express a fluorescent protein, we recommend viewing unstained cells under the FITC channel to examine autofluorescence. For cells expressing a fluorescent protein, we recommend analyzing unstained cell samples to determine the extent the fluorescent protein emission may overlap in the FITC channel.

    Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

    Our Image-iT Green Hypoxia Reagent is already highly fluorescent before using the product. What could have caused this?

    This could be due to storage of the product under anaerobic or low oxygen conditions. The Image-iT Green Hypoxia Reagent increases in fluorescence upon exposure to low oxygen environments, and this change is not reversible. For some ROS indicators, we recommend storing the reagent under dry nitrogen or argon to prevent oxidation during storage. However, this is not appropriate for the Image-iT Green Hypoxia Reagent.

    Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

    What is different between the use of the Hypoxia Green Reagent for Flow Cytometry (Cat No. H20035) versus imaging using the Image-iT Green Hypoxia Reagent (Cat Nos. I14833, I14834)?

    The difference between these products is in the final working concentration of the reagent and incubation time. For flow cytometry applications, we recommend using a final concentration in the range of 0.5 to 1 µM with an incubation time ranging from 2 to 3 hrs. For imaging, we recommend a final concentration in the range of 1 to 10 µM with an incubation time ranging from 30 mins to 1 hr. For both applications, one should optimize the final working concentration and incubation time.

    Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

    Is Hypoxia Green Reagent for Flow Cytometry (Cat No. H20035) the same reagent as the Image-iT Green Hypoxia Reagent (Cat Nos. I14833, I14834)?

    The Hypoxia Green Reagent for Flow Cytometry and the Image-iT Green Hypoxia Reagent are the same reagent, formulated differently for flow cytometry and imaging use, respectively.

    Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

    May I include a total cell stain (to stain all cells) along with Image-iT Hypoxia Reagent?

    You may use cell-permeable nuclear counterstains or surface labels, but avoid any general cytoplasmic stains that may interfere with the emission of the hypoxia reagents.

    Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

    引用および参考文献 (1)

    引用および参考文献
    Abstract
    Restricted exchange microenvironments for cell culture.
    Authors:Hoh JH, Werbin JL, Heinz WF,
    Journal:Biotechniques
    PubMed ID:29570441
    'Metabolite diffusion in tissues produces gradients and heterogeneous microenvironments that are not captured in standard 2D cell culture models. Here we describe restricted exchange environment chambers (REECs) in which diffusive gradients are formed and manipulated on length scales approximating those found in vivo. In REECs, cells are grown in 2D ... More