Vital staining of cardiac myocytes during embryonic stem cell cardiogenesis in vitro.
Authors:Metzger JM, Lin WI, Samuelson LC,
Journal:Circ Res
PubMed ID:8635211
'Mouse embryonic stem (ES) cells differentiate in vitro into a variety of cell types, including spontaneously contracting cardiac myocytes. The primary aim of this work was to use vital stain techniques for real-time detection of developing cardiac myocytes in ES cell differentiation cultures. The -440 to +6 human cardiac alpha-actin ... More
Activity of Hb9 interneurons during fictive locomotion in mouse spinal cord.
'Hb9 interneurons (Hb9 INs) are putative components of the mouse spinal locomotor central pattern generator (CPG) and candidates for the rhythm-generating kernel. Studies in slices and hemisected spinal cords showed that Hb9 INs display TTX-resistant membrane potential oscillations, suggesting a role in rhythm generation. To further investigate the roles of ... More
Functional ephrin-B2 expression for promotive interaction between arterial and venous vessels in postnatal neovascularization.
'Ephrin-B2, one of the transmembrane ligands, is a genetic marker of arterial endothelial cells (ECs) at embryonic stages and is essential for cardiovascular development, but its roles in ischemic cardiovascular disease are not well understood. In this study, we focused on the function of ephrin-B2 in postnatal neovascularization. We found ... More
Fluorescence-activated cell sorting (FACS) of Drosophila hemocytes reveals important functional similarities to mammalian leukocytes.
Authors:Tirouvanziam R, Davidson CJ, Lipsick JS, Herzenberg LA
Journal:Proc Natl Acad Sci U S A
PubMed ID:14976247
Drosophila is a powerful model for molecular studies of hematopoiesis and innate immunity. However, its use for functional cellular studies remains hampered by the lack of single-cell assays for hemocytes (blood cells). Here we introduce a generic method combining fluorescence-activated cell sorting and nonantibody probes that enables the selective gating ... More