Image-IT™ LIVE Mitochondrial Transition Pore Assay Kit, for microscopy

Citations & References (14)

Invitrogen™

Image-IT™ LIVE Mitochondrial Transition Pore Assay Kit, for microscopy

Image-IT™ LIVEミトコンドリア遷移孔アッセイキットは、ミトコンドリア透過性遷移孔開口度測定の実証済みメソッド[REF PN36389、PN32679]を提供します。このキットには色素とクエンチャーが含まれており、細胞に簡単にロードできます。健康な細胞では、色素はミトコンドリアに入りますが、クエンチャーは入りません詳細を見る

製品番号（カタログ番号）	数量
I35103	1 Kit

製品番号（カタログ番号） I35103

価格（JPY）

159,300

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1 Kit

Image-IT™ LIVEミトコンドリア遷移孔アッセイキットは、ミトコンドリア透過性遷移孔開口度測定の実証済みメソッド[REF PN36389、PN32679]を提供します。このキットには色素とクエンチャーが含まれており、細胞に簡単にロードできます。健康な細胞では、色素はミトコンドリアに入りますが、クエンチャーは入りません。ミトコンドリアは、ミトコンドリアの細孔活性化により蛍光が消光されるまで明るく蛍光を維持します。

研究用にのみ使用できます。診断用には使用いただけません。

色オレンジ, 青色, 緑色

使用対象 (装置)蛍光顕微鏡

製品ラインImage-iT

製品タイプMitochondrial Transition Pore Assay Kit

数量1 Kit

出荷条件室温

細胞内局在ミトコンドリア

フォーマットキット

Unit SizeEach

組成および保存条件

フリーザー（-5～-30度）に保存し、遮光してください。

引用および参考文献 (14)

引用および参考文献

Abstract

Transient and long-lasting openings of the mitochondrial permeability transition pore can be monitored directly in intact cells by changes in mitochondrial calcein fluorescence.

Authors:Petronilli V, Miotto G, Canton M, Brini M, Colonna R, Bernardi P, Di Lisa F

Journal:Biophys J

PubMed ID:9929477

'The occurrence and the mode of opening of the mitochondrial permeability transition pore (MTP) were investigated directly in intact cells by monitoring the fluorescence of mitochondrial entrapped calcein. When MH1C1 cells and hepatocytes were loaded with calcein AM, calcein was also present within mitochondria, because (i) its mitochondrial signal was ... More

Effect of transient and permanent permeability transition pore opening on NAD(P)H localization in intact cells.

Authors:Dumas JF, Argaud L, Cottet-Rousselle C, Vial G, Gonzalez C, Detaille D, Leverve X, Fontaine E,

Journal:J Biol Chem

PubMed ID:19346250

'To study the effect of mitochondrial permeability transition pore (PTP) opening on NAD(P)H localization, intact cells were exposed to the Ca(2+) ionophore A23187. PTP opening, mitochondrial membrane potential, mitochondrial volume, and NAD(P)H localization were assessed by time-lapse laser confocal microscopy using the calcein-cobalt technique, tetramethylrhodamine methyl ester, MitoTracker, and NAD(P)H ... More

Measuring apoptosis at the single cell level.

Authors:Bouchier-Hayes L, Muñoz-Pinedo C, Connell S, Green DR,

Journal:Methods

PubMed ID:18314052

The use of live cell microscopy has made a number of contributions to the study of apoptosis. Many of the tools and techniques are available that allow us to image the key events that occur during cell death including mitochondrial outer membrane permeabilization, mitochondrial transmembrane potential changes, translocation of Bcl-2 ... More

The mechanisms of apoptosis in biology and medicine: a new focus for ophthalmology.

Authors:Tempestini A, Schiavone N, Papucci L, Witort E, Lapucci A, Cutrì M, Donnini M, Capaccioli S

Journal:Eur J Ophthalmol

PubMed ID:12749672

Defects in apoptosis (programmed cell death) have recently emerged as being closely involved in the pathogenesis of most ocular diseases and, therefore, apoptosis is now a topic of exponential interest in ophthalmology. This review summarizes recent works on mechanisms of apoptosis, from its initiation and modulation to the switching-on of ... More

Therapeutic Potential of a Novel Necrosis Inhibitor, 7-Amino-Indole, in Myocardial Ischemia-Reperfusion Injury.

Authors:

Journal:Hypertension

PubMed ID:29661840

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