iBlot™ 2 Transfer Stacks, nitrocellulose, regular size
Invitrogen™

iBlot™ 2 Transfer Stacks, nitrocellulose, regular size

iBlot 2 Transfer Stacks are used to transfer proteins using the iBlot 2 Gel Transfer Device. They are consumable stacks with an integrated nitrocellulose transfer membrane for dry blotting of proteins.
製品番号(カタログ番号)数量
IB2300110 Stacks
製品番号(カタログ番号) IB23001
価格(JPY)
38,900
Each
数量:
10 Stacks

iBlot 2 Transfer Stacks are used to transfer proteins using the iBlot 2 Gel Transfer Device. They are consumable stacks with an integrated nitrocellulose transfer membrane for dry blotting of proteins. Each iBlot 2 Transfer Stack contains a copper-coated electrode and appropriate cathode and anode buffers in the gel matrix to allow fast, reliable transfer of proteins. This regular-size stack (13 x 8.3 cm) can be used with midi-size gels or two mini-size gels or E-PAGE 48 or 96 gels.

Nitrocellulose membrane
The nitrocellulose membrane (0.2-μm pore size) in this stack is composed of 100% pure nitrocellulose to provide high-quality transfer. The membrane is compatible with commonly used detection methods such as staining, immunodetection, fluorescence, and radiolabeling. The proteins bind to the membrane due to hydrophobic and electrostatic interactions. The protein-binding capacity is 209 μg/cm2.

iBlot 2転写スタックは、iBlot 2ゲル転写装置を使用したタンパク質の転写に使用します。このスタックは、タンパク質のドライブロッティングに使用する一体型ニトロセルロース転写膜を収めた消耗品スタックです。iBlot 2転写スタックのそれぞれでは、銅皮膜電極および適切な陰極バッファーと陽極バッファーがゲルマトリックスに収められ、タンパク質の迅速で信頼性に優れた転写を可能にしています。この標準サイズ(13 cm x 8.3 cm)のスタックは、ミディサイズゲル、2枚のミニサイズゲル、あるいはE-PAGE 48ゲルまたは96ゲルに使用できます。

iBlot 2システムの詳細を見る›

ニトロセルロース膜
このスタックのニトロセルロース膜(孔径0.2 µm)は、純粋なニトロセルロースを材質として高品質な転写を実現します。この膜は、染色、免疫検出、蛍光、放射標識などの一般的に使用される検出法に適合します。タンパク質は、疎水性相互作用と静電的相互作用によって膜に結合します。このタンパク質結合能は209 µg/cm2です。

研究用途にのみご使用ください。診断目的には使用できません。
仕様
数量10 Stacks
Dimensions (LxW)標準サイズ、13.5 x 8 cm
フォーマット転写スタック
長さ(メートル法)13.5 cm
材料ニトロセルロース膜
孔径0.2 μm
製品ラインiBlot
対応可能10 Blots
幅(メートル法)8 cm
Unit SizeEach
組成および保存条件
室温保存。

よくあるご質問(FAQ)

If a green color appears on the membrane after the transfer using the iBlot 2 Dry Blotting System, will it affect the results?

The green discoloration is copper deposits from the transfer stack, and it does not affect the results. To minimize this effect, shake excess water off the filter paper and buffer from the gel before placing each on the stack. The current formulation of stacks minimizes the green discoloration.

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

Can I cut the bottom stack of my iBlot 2 Transfer Stack to fit my separating gel?

No. Do not trim the iBlot 2 Transfer Stacks to fit your gel size.

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

Is the PVDF membrane in the iBlot 2 PVDF Gel Transfer Stack pre-activated?

The PVDF membrane is pre-activated and ready for use without any pre-treatment with alcohol.

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

Can I use the stack and/or absorbent pad more than once when using the iBlot 2 Dry Blotting System?

No. The transfer stacks have a finite amount of ions to drive the transfer and are depleted after a single use.

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

Can I perform multiple transfers, with a new transfer starting immediately after the previous one when using the iBlot 2 Dry Blotting System?

Yes. The device is designed to do so with no impact on performance.

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.