iBlot™ 2 Transfer Stacks, PVDF, regular size
iBlot™ 2 Transfer Stacks, PVDF, regular size
Invitrogen™

iBlot™ 2 Transfer Stacks, PVDF, regular size

Green features
iBlot 2 Transfer Stacks are used to transfer proteins using the iBlot 2 Gel Transfer Device. iBlot 2 Transfer Stacks詳細を見る
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製品番号（カタログ番号）数量
IB2400110 Stacks
製品番号（カタログ番号） IB24001
価格（JPY）
40,100
Each
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数量:
10 Stacks

iBlot 2 Transfer Stacks are used to transfer proteins using the iBlot 2 Gel Transfer Device. iBlot 2 Transfer Stacks are consumable stacks with an integrated pre-activated PVDF transfer membrane for dry blotting of proteins. Each iBlot 2 Transfer Stack contains a copper-coated electrode and appropriate cathode and anode buffers in the gel matrix to allow fast, reliable transfer of proteins. This regular-size stack (13 x 8.3 cm) can be used with midi-size gels or two mini-size gels or E-PAGE 48 or 96 gels.

PVDF membrane

The PVDF membrane (0.2-μm pore size, low fluorescence) in this stack has higher binding capacity than nitrocellulose. It is pre-activated and ready for use without any pretreatment with alcohols. The membrane is compatible with commonly used detection methods such as staining, immunodetection, fluorescence, and radiolabeling. The proteins bind to the membrane due to hydrophobic interactions. The protein-binding capacity is 240 μg/cm2.

研究用途にのみご使用ください。診断目的には使用できません。
仕様
グリーン機能持続可能な廃棄
数量10 Stacks
Dimensions (LxW)標準サイズ、13.5 x 8 cm
フォーマット転写スタック
長さ（メートル法）13.5 cm
材料PVDF膜
孔径0.2 μm
製品ラインiBlot
対応可能10 Blots
幅（メートル法）8 cm
Unit SizeEach
組成および保存条件
室温保存。

よくあるご質問（FAQ）

If a green color appears on the membrane after the transfer using the iBlot 2 Dry Blotting System, will it affect the results?

The green discoloration is copper deposits from the transfer stack, and it does not affect the results. To minimize this effect, shake excess water off the filter paper and buffer from the gel before placing each on the stack. The current formulation of stacks minimizes the green discoloration.

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

Can I cut the bottom stack of my iBlot 2 Transfer Stack to fit my separating gel?

No. Do not trim the iBlot 2 Transfer Stacks to fit your gel size.

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

Is the PVDF membrane in the iBlot 2 PVDF Gel Transfer Stack pre-activated?

The PVDF membrane is pre-activated and ready for use without any pre-treatment with alcohol.

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

Can I use the stack and/or absorbent pad more than once when using the iBlot 2 Dry Blotting System?

No. The transfer stacks have a finite amount of ions to drive the transfer and are depleted after a single use.

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

Can I perform multiple transfers, with a new transfer starting immediately after the previous one when using the iBlot 2 Dry Blotting System?

Yes. The device is designed to do so with no impact on performance.

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

View all iBlot™ 2 Transfer Stacks, PVDF, regular size, 10 Stacks FAQs