Invivofectamine™ 3.0 Reagent

Isolating high-quality RNA is critical to the overall success of in vivo experiments.

Invivofectamine 3.0 Reagent is designed to be delivered slowly and evenly; a 200-µL injection should take between 10 to 20 seconds.

Sterile technique is vital to successful completion of in vivo experiments. All materials need to be sterile before injecting the complexed siRNA into animals.

The protocol is robust but specific. Protocol modifications will likely result in less than optimal performance.

Both positive and negative controls are recommended when performing any siRNA experiment. We typically use Invitrogen in vivo siRNA against Factor VII with Invivofectamine 3.0 Reagent, as a positive control for experiments. It is also good practice to use PBS solution as a negative control.