Anza™ 13 Esp3I

All FastDigest enzymes are active in Anza buffer. Therefore, a double digest with Anza 13 Esp3I (Cat. No. IVGN0136) and FastDigest SgsI (Cat. No. FD1894) should be performed in the Anza buffer. For a reaction mix of 20 µL, we recommend combining 1 µL of each enzyme with 2 µL of the 10x Anza buffer and up to 1 µg of DNA.

All Anza restriction enzymes contain BSA.

Enzymes may freeze during shipment on dry ice. This does not affect them as they have been tested and shown to be 100% active after three freeze-thaw cycles. For 24-48 hour delivery, enzymes may be shipped on ice pack as their performance is not affected by short exposure times at 4 degrees C.

For optimal results, we highly recommend exclusively using Anza restriction enzymes in double digestion as they all have 100% buffer compatibility. If a certain enzyme is not available in Anza format, but is available in FastDigest format or as a Thermo Scientific conventional restriction enzyme, it may be possible to perform a double digestion. For specific protocol recommendations, please contact our technical support with detailed information of the desired enzymes and DNA template.

When the next step following digestion is agarose gel electrophoresis, the Anza Red buffer can be used directly. The Anza Red buffer contains the necessary loading dye and density gradient for directly loading on to the agarose gel. The red dye does not interfere with digestion and helps reduce additional pipetting.

Use the clear Anza Buffer for applications that require analysis by fluorescence excitation, as the dyes in the Anza Red Buffer may interfere with some fluorescence measurements.