General Description

• Laemmli SDS sample buffer is composed of several compounds, including 375 mM Tris-HCl (pH 6.8), 9% SDS, 50% glycerol, 6% β-mercaptoethanol, 0.03% bromophenol blue
• The SDS compound present in the Laemmli SDS sample buffer binds noncovalently to proteins. SDS is negatively charged and can mask the intrinsic charge of the protein it binds

Application

• Laemmli SDS sample buffer is used to denature proteins and for protein sample preparation to be used in the SDS-PAGE system
• This sample buffer is used as an electrophoretic dye for denaturation of proteins and monitoring the front of the running gel
• It is also used for polyacrylamide protein gel analysis