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Thermo Scientific Chemicals
Guanidine hydrochloride, Molecular Biology Grade
A protein denaturant | CAS: 50-01-1 | CH6ClN3 | 95.53 g/mol
化学物質識別子
CAS50-01-1
IUPAC Namehydrogen guanidine chloride
Molecular FormulaCH6ClN3
InChI KeyPJJJBBJSCAKJQF-UHFFFAOYSA-N
SMILES[H+].[Cl-].NC(N)=N
さらに表示
仕様
スペックシート
スペックシートAssay (unspecified)Absorbance (260nm, 6M in water): 0.03 max.
Appearance (Color)White
FormPowder
Assay (Titration)99.5% min.
DNaseNone detected
さらに表示
Guanidine hydrochloride is used for the denaturation and subsequent refolding of proteins. This strong denaturant can solubilize insoluble or denatured proteins such as inclusion bodies. This can be used as the first step in refolding proteins or enzymes into their active form. Urea and dithiothreitol (DTT) may also be necessary. Guanidine hydrochloride is used in RNA isolation to dissociate nucleoproteins and inhibit RNase.
This Thermo Scientific Chemicals brand product was originally part of the Alfa Aesar product portfolio. Some documentation and label information may refer to the legacy brand. The original Alfa Aesar product / item code or SKU reference has not changed as a part of the brand transition to Thermo Scientific Chemicals.
Applications
Guanidine hydrochloride is used for the denaturation and subsequent refolding of proteins. This strong denaturant can solubilize insoluble or denatured proteins such as inclusion bodies. This can be used as the first step in refolding proteins or enzymes into their active form. Urea and dithiothreitol (DTT) may also be necessary. Guanidine hydrochloride is used in RNA isolation to dissociate nucleoproteins and inhibit RNase.
Solubility
Freely soluble in water
Notes
Protect from heat. Store away from oxidizing agents.
Guanidine hydrochloride is used for the denaturation and subsequent refolding of proteins. This strong denaturant can solubilize insoluble or denatured proteins such as inclusion bodies. This can be used as the first step in refolding proteins or enzymes into their active form. Urea and dithiothreitol (DTT) may also be necessary. Guanidine hydrochloride is used in RNA isolation to dissociate nucleoproteins and inhibit RNase.
Solubility
Freely soluble in water
Notes
Protect from heat. Store away from oxidizing agents.
RUO – Research Use Only
General References:
- Dipti Arora.; Navin Khanna. Method for increasing the yield of properly folded recombinant human gamma interferon from inclusion bodies. Journal of Biotechnology. 1996, 52, (2), 127-133.
- Cing-Yuen Won.; Maurice R. Eftin. Incorporation of Tryptophan Analogues into Staphylococcal Nuclease, Its V66W Mutant, and Δ137-149 Fragment: Spectroscopic Studies. Biochemistry. 1998, 37, (25), 8938-8946.