PureLink™ FFPE RNA Isolation Kit

Since the quality of RNA is dependent on the quality of FFPE tissues itself, the amplifiable cDNA length is variable. Competitors' kits have been shown to max out around 400 bp, while in our experience, with our patent-pending method in the PureLink FFPE RNA Isolation Kit, most RT-PCR products are about 400 bp to 800 bp, and can even be over 1.3 kb.

The PureLink FFPE RNA Isolation Kit provides a simple and rapid method for isolating total RNA from formalin-fixed paraffin-embedded (FFPE) samples with high-quality results. A fast and easy heating procedure replaces traditional labor-intensive protocols that use toxic organic solvents for deparaffinization and give disappointing results. This reduces the time required for total RNA isolation to 30-40 minutes instead of the hours or a full day required by traditional FFPE isolation methods. In addition, the RNA isolated with the PureLink FFPE RNA Isolation Kit is of higher quality and demonstrates increased amplifiable RNA size, allowing for RT-PCR products that are longer than those obtained with competitor kits and reagents. Isolated RNA is ideal for use in all gene expression studies, including microarrays, RT-PCR, and qRT-PCR.