S.N.A.P.™ミニプレップスピンカラム、溶解バッファー、結合バッファー、洗浄バッファー、4X 最終洗浄、および収集チューブを室温で使用できます。再懸濁バッファー、沈殿塩溶液、RNase A を +4 ℃ で保存しますすべての試薬は、適切な保管条件であれば、6カ月間安定していることが保証されています。
よくあるご質問(FAQ)
What is the upper size limit for plasmid purification with the S.N.A.P. Miniprep Kit?
Bacmid DNA (>100 kbp) has been purified with the S.N.A.P. miniprep kit.
The PureLink HiPure Plasmid DNA Purification Kits (mini/midi/maxi scales) can also be used for large constructs. The HiPure kit manuals contain a modified protocol designed to help increase yields with these large plasmid isolations.
What are the compositions of the S.N.A.P. MiniPrep and MidiPrep Kit solutions?
The formulations are published in the manual for the S.N.A.P. MidiPrep kit, and are the same as the ones used in the MiniPrep kit.
What is the resin in the S.N.A.P. kits?
The column contains a silica/glass fiber based resin. However, the exact composition is proprietary.
Why is LB medium recommended for growing up plasmid preps in the S.N.A.P. protocol?
The volumes of culture recommended in our S.N.A.P. kits assume an OD of no more than 2.0. If a rich medium is used, the volume processed should be reduced to avoid overloading the column.
Why can't I dissolve all of my DNA after precipitation?
Sometimes there is insoluble material (probably resin fines) in the precipitated DNA. This material is very easily removed with a 10 second spin. The presence of these particles does not reflect a poor preparation. Once the particles are removed, the DNA performs well in all applications.