S.N.A.P.™ミニプレップフィルターカラム、S.N.A.P.™ミニプレップDNA 結合カラム、溶解バッファー、結合バッファー、洗浄バッファー、および室温での 4 倍最終洗浄。再懸濁液バッファー、沈殿塩、RNase A を +4 ℃ で保存しますすべての試薬は、適切な保管条件であれば、6カ月間安定していることが保証されています。
よくあるご質問(FAQ)
Which centrifuge is used at Thermo Fisher Scientific for the S.N.A.P. MidiPrep protocol?
The Sorval RT6000B table-top centrifuge is used at Thermo Fisher Scientific for the S.N.A.P. MidiPrep protocol. The low-speed spins are around 2,000 rpm and the high-speed spins are around 3,000 rpm. You may also use the Sorval RC-5B centrifuge with the SS-34 rotor. However, you might observe some spillage with this centrifuge.
What is the upper size limit for plasmid purification with the S.N.A.P. Miniprep Kit?
Bacmid DNA (>100 kbp) has been purified with the S.N.A.P. miniprep kit.
The PureLink HiPure Plasmid DNA Purification Kits (mini/midi/maxi scales) can also be used for large constructs. The HiPure kit manuals contain a modified protocol designed to help increase yields with these large plasmid isolations.
What are the compositions of the S.N.A.P. MiniPrep and MidiPrep Kit solutions?
The formulations are published in the manual for the S.N.A.P. MidiPrep kit, and are the same as the ones used in the MiniPrep kit.
What is the resin in the S.N.A.P. kits?
The column contains a silica/glass fiber based resin. However, the exact composition is proprietary.
Why is LB medium recommended for growing up plasmid preps in the S.N.A.P. protocol?
The volumes of culture recommended in our S.N.A.P. kits assume an OD of no more than 2.0. If a rich medium is used, the volume processed should be reduced to avoid overloading the column.