Citations & References (4)

Invitrogen™

Vivid Colors™ pcDNA™6.2/C-EmGFP-GW/TOPO™ Mammalian Expression Vector

Vivid Colors™ pcDNA™6.2 Fluorescent Protein TOPO™発現ベクター（図1）を使用すると、迅速に遺伝子をクローニングし、その遺伝子を詳細を見る

製品番号（カタログ番号）	数量
K35920	20 reactions

製品番号（カタログ番号） K35920

価格（JPY）

272,800

20 reactions

Vivid Colors™ pcDNA™6.2 Fluorescent Protein TOPO™発現ベクター（図1）を使用すると、迅速に遺伝子をクローニングし、その遺伝子を、幅広く使用されているオワンクラゲ（1、2）の非常に特徴的な蛍光タンパク質（FP）に融合させることができます。これらの強力なTOPO™クローニングベクターには、組換えタンパク質を簡単かつ非侵襲的に検出するためにEmerald Green Fluorescent Protein（EmGFP）またはYellow Fluorescent Protein（YFP）が含まれています（図2）。どちらのFPも、最適な哺乳類発現のためにヒト化されています（3）。Vivid Colors™ pcDNA™6.2 Fluorescent Protein TOPO™発現ベクターの特長：
• 目的のPCR増幅遺伝子のための1ステップ、5分間のTOPO™クローニング用のトポイソメラーゼI
• 組換え蛍光融合タンパク質の高レベル発現用CMVプロモーター
• N末端またはC末端へのEmGFPまたはYFPの融合能力
• 安定した細胞株を迅速に選択するためのBSD耐性マーカー

研究用にのみ使用できます。診断用には使用いただけません。

構成または誘導システム構造的

供給タイプTransfection

使用対象（アプリケーション）レポーターアッセイ、細胞成分の局在性

製品タイプ哺乳類発現用ベクター

数量20 reactions

レポーター遺伝子GFP（EmGFP）

選択剤（真核生物）ブラストサイジン

ベクターpcDNA

クローニング法TOPO™とGateway™

製品ラインGateway、TOPO、Vivid Colors、pcDNA, TOPO, Vivid Colors, pcDNA

プロモーターCMV

タンパク質タグGFP（EmGFP）

Unit SizeEach

組成および保存条件

各pcDNA™6.2 TOPO™ Expression Vector Kitには2つのボックスが含まれています。TOPO™ボックスには、線直線化されたトポイソメラーゼI活性化pcDNA™ 6.2 TOPO™ベクター、コントロールテンプレート、シーケンシング用プライマー、PCR反応要素、および発現コントロールプラスミドが含まれています。TOPO™ボックスは-20℃で保管してください。One Shot™ボックスには、One Shot™ TOP10ケミカルコンピテント大腸菌の使い切りの50µLのアリコートなどの形質転換試薬、S.O.C.培地、pUC19超らせん型プラスミドコントロールが含まれています。コンピテント大腸菌を-80℃で保管してください。すべての試薬は、適切な保管条件であれば、6カ月間安定していることが保証されています。

よくあるご質問（FAQ）

Your Gateway-adapted TOPO vectors are supplied with a control template and control primers. Can I obtain the sequence of the control template?

The sequence of the control template is proprietary.

Can GFP fluorescence be detected in cells that have been stained for beta-galactosidase?

We recommend looking for GFP fluorescence before staining for beta-galactosidase. This is because the beta-galactosidase staining process produces a very high autofluorescence that will interfere with detection of GFP fluorescence.

What are the recommended filter sets for detection of EmGFP, YFP, CFP, and BFP by fluorescence microscopy?

EmGFP, YFP, CFP, and BFP can be detected using standard FITC filter sets and settings. However, for optimal detection of the fluorescence signal, filter sets optimized for detection within the excitation and emission ranges for each fluorescent protein are recommended. The recommended filter sets are as follows: EmGFP: Omega filter set XF100 YFP: Omega filter set XF1042 Chroma filter set 41028 CFP: Omega filter set XF114 Chroma filter set 31044 BFP: Omega filter set XF10 Chroma filter set 31021 For information on obtaining filter sets, please contact Omega Optical, Inc. (www.omegafilters.com) or Chroma Technology Corporation (www.chroma.com) directly.

What are the excitation and emission maxima for your fluorescent proteins (EmGFP, YFP, BFP, CFP, and Cycle 3 GFP)?

Excitation and emission maxima for our fluorescent proteins are as follows:
- EmGFP: Excitation: 487 nm; Emission: 509 nm
- YFP: Excitation: 514 nm; Emission: 527 nm
- BFP: Excitation: 308-383 nm; Emission: 440-447 nm
- CFP: Excitation: 452 nm; Emission: 505 nm
- Cycle 3 GFP: Primary excitation: 395 nm; Secondary Excitation: 478 nm; Emission: 507 nm

Are the fluorescent proteins you offer (EmGFP, YFP, CFP, BFP, and Cycle 3 GFP) humanized?

Yes, all of the fluorescent proteins offered by us (EmGFP, YFP, CFP, BFP, and Cycle 3 GFP) have been humanized for optimal mammalian expression.

View all Vivid Colors™ pcDNA™6.2/C-EmGFP-GW/TOPO™ Mammalian Expression Vector FAQs

引用および参考文献 (4)

引用および参考文献

Abstract

Ultra-high-throughput screening method for the directed evolution of glucose oxidase.

Authors:Ostafe R, Prodanovic R, Nazor J, Fischer R,

Journal:

PubMed ID:24613019

'Glucose oxidase (GOx) is used in many industrial processes that could benefit from improved versions of the enzyme. Some improvements like higher activity under physiological conditions and thermal stability could be useful for GOx applications in biosensors and biofuel cells. Directed evolution is one of the currently available methods to ... More

Development of a fluorescence-based method for monitoring glucose catabolism and its potential use in a biomass hydrolysis assay.

Authors:Haney LJ, Coors JG, Lorenz AJ, Raman DR, Anex RP, Scott MP,

Journal:Biotechnol Biofuels

PubMed ID:19019221

'The availability and low cost of lignocellulosic biomass has caused tremendous interest in the bioconversion of this feedstock into liquid fuels. One measure of the economic viability of the bioconversion process is the ease with which a particular feedstock is hydrolyzed and fermented. Because monitoring the analytes in hydrolysis and ... More

Lignin depletion enhances the digestibility of cellulose in cultured xylem cells.

Authors:Lacayo CI, Hwang MS, Ding SY, Thelen MP,

Journal:

PubMed ID:23874568

'Plant lignocellulose constitutes an abundant and sustainable source of polysaccharides that can be converted into biofuels. However, the enzymatic digestion of native plant cell walls is inefficient, presenting a considerable barrier to cost-effective biofuel production. In addition to the insolubility of cellulose and hemicellulose, the tight association of lignin with ... More

Surface carbohydrate analysis and bioethanol production of sugarcane bagasse pretreated with the white rot fungus, Ceriporiopsis subvermispora and microwave hydrothermolysis.

Authors:Sasaki C, Takada R, Watanabe T, Honda Y, Karita S, Nakamura Y, Watanabe T,

Journal:Bioresour Technol

PubMed ID:21903385

'Effects of pretreatments with a white rot fungus, Ceriporiopsis subvermispora, and microwave hydrothermolysis of bagasse on enzymatic saccharification and fermentation were evaluated. The best sugar yield, 44.9 g per 100g of bagasse was obtained by fungal treatments followed by microwave hydrothermolysis at 180°C for 20 min. Fluorescent-labeled carbohydrate-binding modules which ... More