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Invitrogen™
ViraPower™ UbC Lentiviral Gateway™ Expression Kit
The ViraPower™ UbC Lentiviral Gateway™ Expression Kit includes all the components needed to generate lentivirus, including vector kit, 293FT cell詳細を見る
| 製品番号（カタログ番号） | 数量 |
|---|---|
| K499000 | 1 kit |
製品番号（カタログ番号） K499000
価格（JPY）お問い合わせください ›
531,600
Each
数量:
1 kit
The ViraPower™ UbC Lentiviral Gateway™ Expression Kit includes all the components needed to generate lentivirus, including vector kit, 293FT cell line, and the support kit. This kit combines Invitrogen™’s ViraPower™ Lentiviral and Gateway™ technologies to facilitate easy recombination-based cloning and lentiviral-based expression of a target gene in dividing and non-dividing mammalian cells. The pLenti6⁄UbC⁄V5-DEST™ vector has the UbC promoter for driving constitutive but physiological levels of expression of the target gene and the blasticidin selection marker for stable selection in mammalian cells.
Advantages
• Stable expression
• Long-term experiments
• Accurate titer of functional virus
• Flexible and versatile Gateway™ recombination cloning technology
Key Features
• UbC promoter
• V5 epitope tag at C terminus
• Blasticidin selection
Kit includes
• pLenti6⁄UbC⁄V5-DEST™ Gateway™ Vector (V499-10)
• ViraPower™ Bsd Lentiviral Support Kit (K4970-00)
• 293FT Cell Line (R700-07)
For research use only. Not intended for any therapeutic or diagnostic use.
Advantages
• Stable expression
• Long-term experiments
• Accurate titer of functional virus
• Flexible and versatile Gateway™ recombination cloning technology
Key Features
• UbC promoter
• V5 epitope tag at C terminus
• Blasticidin selection
Kit includes
• pLenti6⁄UbC⁄V5-DEST™ Gateway™ Vector (V499-10)
• ViraPower™ Bsd Lentiviral Support Kit (K4970-00)
• 293FT Cell Line (R700-07)
For research use only. Not intended for any therapeutic or diagnostic use.
研究用途にのみご使用ください。診断目的には使用できません。
仕様
構成または誘導システム構造的
供給タイプLentiviral
使用対象（アプリケーション）ウイルス発現
製品タイプLentiviral Expression Kit
数量1 kit
選択剤（真核生物）ブラストサイジン
ベクターpLenti
クローニング法Gateway
製品ラインGateway, ViraPower
プロモーターUbC
タンパク質タグV5 Epitope Tag
Unit SizeEach
組成および保存条件
Vector kit (Boxes 1 and 2)
pLenti6⁄UbC⁄V5-DEST™ (Box 1): 6 μg, -20°C
pLenti6⁄UbC⁄V5-GW⁄lacZ Control vector (Box 1): 10 μg, -20°C
One Shot™ Stbl3™ Chemically Competent E. coli (Box 2): 21 x 50 μL, -80°C
pUC19 control DNA (10 pg⁄μL): 50 μL, -80°C
S.O.C. Medium: 6 mL -80°C
Support kit (Box 3)
ViraPower™ Packaging Mix (1 μg⁄μL): 195 μg, -20°C
Lipofectamine™ 2000: 0.75 mL, +4°C
Blasticidin: 50 mg, -20°C
293 FT cells (3 x 106⁄mL) (Box 4): 1 mL, liquid nitrogen
pLenti6⁄UbC⁄V5-DEST™ (Box 1): 6 μg, -20°C
pLenti6⁄UbC⁄V5-GW⁄lacZ Control vector (Box 1): 10 μg, -20°C
One Shot™ Stbl3™ Chemically Competent E. coli (Box 2): 21 x 50 μL, -80°C
pUC19 control DNA (10 pg⁄μL): 50 μL, -80°C
S.O.C. Medium: 6 mL -80°C
Support kit (Box 3)
ViraPower™ Packaging Mix (1 μg⁄μL): 195 μg, -20°C
Lipofectamine™ 2000: 0.75 mL, +4°C
Blasticidin: 50 mg, -20°C
293 FT cells (3 x 106⁄mL) (Box 4): 1 mL, liquid nitrogen