ViraPower™ HiPerform™ Lentiviral TOPO™ Expression Kit
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Invitrogen™

ViraPower™ HiPerform™ Lentiviral TOPO™ Expression Kit

導入が困難な哺乳類細胞または動物モデルのどちらを使用する場合でも、あるいは増殖または薬物停止細胞、初代細胞、または幹細胞への効率的な遺伝子デリバリーを必要とする場合でも、レンチウイルスシステムは安定した遺伝子発現に最適です。Invitrogen社のViraPower™ HiPerform™レンチウイルス発現キットは、改善されたpLentiベクター、およびウイルス作成に必要なすべてのものを含む、利便性の高いキットです詳細を見る
製品番号(カタログ番号)数量
K5310001 kit
製品番号(カタログ番号) K531000
価格(JPY)
618,500
Each
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数量:
1 kit
導入が困難な哺乳類細胞または動物モデルのどちらを使用する場合でも、あるいは増殖または薬物停止細胞、初代細胞、または幹細胞への効率的な遺伝子デリバリーを必要とする場合でも、レンチウイルスシステムは安定した遺伝子発現に最適です。

Invitrogen社のViraPower™ HiPerform™レンチウイルス発現キットは、改善されたpLentiベクター、およびウイルス作成に必要なすべてのものを含む、利便性の高いキットです。

このキットには、次の2つの主要な要素を含む、当社の新しいpLenti6.3⁄V5 TOPO™クローニングベクターが含まれています: HIV-1インテグラーゼ遺伝子からウッドチャック転写後調節エレメント(WPRE)およびcentral polypurine tract(cPPT)配列。 これらの要素は、WPREおよびcPPTエンハンサーが欠如しているベクターと比較して、タンパク質発現において少なくとも4倍増加させます。

このキットの主要な利点には以下が含まれています:
• 安定した発現
• 長期的な実験
• 活性のあるウイルスの正確な力価(Blasticidin法を使用)
• 5分間でライゲーションできる高効率的なTOPO™ PCRクローニング
研究用途にのみご使用ください。診断目的には使用できません。
仕様
製品ラインHiPerform, TOPO, ViraPower
数量1 kit
製品タイプLentiviral Expression Kit
Unit SizeEach
組成および保存条件
TOPO™ TA Cloning Reagents (Box 1)
• pLenti6.3⁄V5-TOPO™ (5-10 ng⁄μL) 20 μL, -20°C
• pLenti6.3⁄V5-GW⁄lacZ Control vector (0.5 ug⁄μL) 20 μL, -20°C
• 10X PCR Buffer 100 μL, -20°C
• l dNTP Mix 10 μL, -20°C
• Salt Solution 50 μL, -20°C
• CMV Forward Primer (100 ng⁄μL) 20 μL, -20°C
• V5 (C-term) Reverse Primer (100 ng⁄μL) 20 μL, -20°C
• Control PCR Template (50 ng⁄μL) 10 μL, -20°C
• Control PCR Primers (100 ng⁄μL) 10 μL each, -20°C
• Sterile Water 1 mL

One Shot™ Stbl3™ Chemically Competent E. coli (Box 2)
• Stbl3™ Cells 21 x 50 μL, -80°C
• pUC19 control DNA (10 pg⁄μL) 50 μL, -80°C
• S.O.C. Medium 6 mL, -80°C

Support kit
• ViraPower™ Packaging Mix 195 μg, -20°C
• Lipofectamine™ 2000 0.75 mL, +4°C
• Blasticidin 50 mg, -20°C
• 293 FT cells (3 x 106⁄mL) 1 mL, liquid nitrogen

よくあるご質問(FAQ)

I used one of your lentiviral vectors but am observing cytotoxic effects after transduction. Can you please help?

Possible causes include:

- large volume of viral supernatant used for transduction
- cells sensitive to Polybrene regaent
- too much antibiotic used for selection
- antibiotic used too soon after tranduction
- gene of interest is toxic to cells

I transduced my lentiviral stock into my mammalian cell line but am getting poor expression of my gene of interest. What could have happened?

Poor expression could result from low transduction efficiency, too low of a MOI, too much antibiotic used for selection, usage of antibiotic too soon after transduction, harveting cells too soon after transduction, having a gene of interest that is toxic to cells, or rerrangement in the LTR regions of the expression construct plasmid DNA.

I transduced my lentiviral stock into my mammalian cell line but am getting no expression of my gene of interest. What could have gone wrong?

Here are some possible causes and solutions:
- Promoter silencing; CMV promoter is prone to silencing especially in mouse and rat cells, screen multiple antibiotic resistant clones and select the one with the highest expression levels
- Viral stocks stored incorrectly; aliquot and store at -80 degrees C, do not freeze/thaw more than 3 times

I prepared a lentiviral stock using one of your lentiviral vectors. I am trying to determine the titer using antibiotic selection but am not able to since the cells are very confluent and I am not getting antibiotic-resistant clones. Can you please offer some tips?

Here are some possible causes and solutions:

- Too little antibotic used for selection
- Selection performed on confluent cells; replate cells
- Viral supernatant not diluted sufficiently; titer lentivus using a wider range of 10-fold serial dilutions

I am using one of your lentiviral vectors and am getting a low lentiviral titer. Can you offer some troubleshooting tips?

Possible causes include:

- low transfection efficiency; Use a high-quality plasmid prep, 293FT cells under passage 16, ensure removal of Geneticin during transfection, ensure correct DNA:lipid ratio, and that cells are plated at the correct confluency
- transfected cells are not cultured in medium containing sodium pyruvate; this reagent provides an extra energy source for cells
- viral supernatant harvested too early; viral supernatants can generally be collected 48-72 hrs post-transfection
- viral supernatant too dilute; concentrate virus using CsCl purification
- viral supernatant frozen and thawed multiple times; 3 times should be the maximum freeze/thaw
- gene of interest is large; viral titers decrease as size of insert increases, inserts larger than 5.6 kb are not recommended
- rearrangement in the LTR region of the epxression construct plasmid DNA; use Stb3 cells for transformatin of the lentiviral construct
- poor choice of titering cell line; use HT1080 cells or similar cell line
- Polybrene reagent is not included during transduction; transduce lentiviral construct into cells in the presence of Polybrene reagent
- Lipofectamine reagent handled incorrectly; ensure proper storage and mix gently before use
- Use fluorescence micrscopy to check titer with HiPerform FastTiter lentivirus

Find additional tips, troubleshooting help, and resources within our Protein Expression Support Center.