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Invitrogen™
ViraPower™ HiPerform™ Lentiviral FastTiter™ Gateway™ Expression Kit
The ViraPower™ HiPerform™ Lentiviral FastTiter™ Gateway™ Expression Kit includes all the components needed to generate lentivirus, including vector kit, 293FT cell line, and the support kit.
| 製品番号（カタログ番号） | 数量 |
|---|---|
| K534000 または、製品番号K5340-00 | 1 Kit |
製品番号（カタログ番号） K534000
または、製品番号K5340-00
価格（JPY）
542,700
Each
数量:
1 Kit
The ViraPower™ HiPerform™ Lentiviral FastTiter™ Gateway™ Expression Kit includes all the components needed to generate lentivirus, including vector kit, 293FT cell line, and the support kit. This kit combines Invitrogen's ViraPower™ HiPerform™ Lentiviral and Gateway™ technologies to facilitate easy recombination-based cloning and lentiviral-based, high-level expression of a target gene in dividing and non-dividing mammalian cells. The pLenti7.3/V5-DEST™ vector is equipped with two key genetic elements, making it a HiPerform™ vector: the Woodchuck Posttranscriptional Regulatory Element (WPRE) and the central Polypurine Tract (cPPT) sequence from the HIV-1 integrase gene to produce at least 4-fold increase in protein expression compared to vectors lacking these elements. In addition, the vector allows for an accurate determination of titer of functional lentivirus in just two days using Emerald Green Fluorescent Protein (EmGFP).
Advantages
- Transient expression
- Short-term experiments
- High-throughput screening
- 2 day titer of functional virus using EmGFP
- Flexible and versatile Gateway™ recombination cloning technology
Key Features
- WPRE (Woodchuck Posttranscriptional Regulatory Element) from the woodchuck hepatitis virus, allows increased transgene expression and cPPT (Polypurine Tract) from the HIV-1 integrase gene, increases the copy number of lentivirus integrating into the host genome
- Human cytomegalovirus (CMV) immediate early promoter to control high-level expression of the gene of interest
- SV40 promoter driving expression of EmGFP
- Emerald Green Fluorescent Protein (EmGFP) allows easy determination of Lentiviral titers by flow cytometry and monitor transduced cells.
Kit includes
- pLenti7.3/V5-DEST™ Gateway™ Vector Kit (Cat. No. V534-06)
- 293FT Cell Line (R700-07)
- ViraPower™ Lentiviral Support Kit (ViraPower™ Lentiviral Packaging Mix and Lipofectamine™ 2000)
研究用途にのみご使用ください。診断目的には使用できません。
仕様
構成または誘導システム構造的
供給タイプLentiviral
使用対象（アプリケーション）レポーターアッセイ、ウイルス発現
製品タイプLentiviral Expression Kit
数量1 Kit
レポーター遺伝子GFP (EmGFP)
選択剤（真核生物）None
特殊元素WPRE、cPPT
ベクターpLenti
クローニング法Gateway
濃度6 μg/μL
製品ラインFastTiter, Gateway, HiPerform, ViraPower
プロモーターCMV
タンパク質タグV5 Epitope Tag
Unit SizeEach
組成および保存条件
pLenti7.3/V5-DEST™ Gateway™ Vector Kit
• pLenti7.3/V5-DEST™ Gateway™ Vector (150 ng/μL), 40 μL
• pLenti7.3/V5-GW/lacZ Control Vector (0.5 μg/μL) , 20 μlL
Store at -20°C.
ViraPower™ Lentiviral Support Kit
Contains enough reagents for 20 transfections in 10 cm tissue culture dishes:
• Lyophilized Packaging Mix, 195 μg, store at -20°C
• Lipofectamine™ 2000, 0.75 mL, store at 4°C
One Shot™ Stbl3™ chemically competent E. coli box
• One Shot™ Competent Cells, 20 single-use aliquots of 50 μL
• S.O.C. medium
• pUC19 supercoiled plasmid control
Store at -80°C.
293FT cell line
• 3 x 106 frozen cells in 1 mL of Freezing Medium, store in liquid nitrogen.