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Invitrogen™

Ni-NTA Purification System with Antibody, with Anti-His (C-term)-HRP Antibody

The Ni-NTA Purification System is designed for the purification of recombinant proteins that contain a polyhistidine (6xHis) sequence. The kit詳細を見る
製品番号(カタログ番号)数量
K95301
または、製品番号K953-01
1キット
製品番号(カタログ番号) K95301
または、製品番号K953-01
価格(JPY)
-
数量:
1キット
一括またはカスタム形式をリクエストする
The Ni-NTA Purification System is designed for the purification of recombinant proteins that contain a polyhistidine (6xHis) sequence. The kit utilizes Ni-NTA nickel-chelating resin and is supplied with native and denaturing buffers for efficient purification of recombinant proteins under different conditions.
For Research Use Only. Not for use in diagnostic procedures.
仕様
数量1キット
タイプProtein Purification System
Unit SizeEach
組成および保存条件
Six 2-ml resin columns and buffers for native and denaturing purification. Ten milliliters of Ni-NTA Agarose. Store at +4°C. All reagents are guaranteed stable for 6 months when properly stored.

よくあるご質問(FAQ)

Can ProBond or Ni-NTA beads be used for large-scale preparations?

ProBond and Ni-NTA beads can be used in FPLC columns. However, the beads can only withstand low pressure (~43.5 psi max).

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

What should the typical protein recovery be when using the Probond Purification System or Ni-NTA Purification system?

Both systems are qualified by purifying 2 mg of myoglobin protein on a column and performing a Bradford assay. Protein recovery must be 75% or higher.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.