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Thermo Scientific Chemicals
Fluorescein isothiocyanate, isomer 1, 95%
CAS: 3326-32-7 | C21H11NO5S | 389.381 g/mol
化学物質識別子
CAS3326-32-7
IUPAC Name3',6'-dihydroxy-5-isothiocyanato-3H-spiro[2-benzofuran-1,9'-xanthen]-3-one
Molecular FormulaC21H11NO5S
InChI KeyMHMNJMPURVTYEJ-UHFFFAOYSA-N
SMILESOC1=CC=C2C(OC3=CC(O)=CC=C3C22OC(=O)C3=CC(=CC=C23)N=C=S)=C1
さらに表示
スペックシートFluorescein isothiocyanate is used as fluorescent labeling reagent for proteins and for rapid identification of pathogens in fluorescent antibody technique. It is also used as a reagent for microsequencing of proteins and peptides (HPLC). On celite it is used for the labeling of fibrinogen.
This Thermo Scientific Chemicals brand product was originally part of the Alfa Aesar product portfolio. Some documentation and label information may refer to the legacy brand. The original Alfa Aesar product / item code or SKU reference has not changed as a part of the brand transition to Thermo Scientific Chemicals.
Applications
Fluorescein isothiocyanate is used as fluorescent labeling reagent for proteins and for rapid identification of pathogens in fluorescent antibody technique. It is also used as a reagent for microsequencing of proteins and peptides (HPLC). On celite it is used for the labeling of fibrinogen.
Solubility
Slightly soluble in DMSO, ethanol, methanol DMF and acetone
Notes
Incompatible with strong oxidizing agents, strong bases and amines.
Fluorescein isothiocyanate is used as fluorescent labeling reagent for proteins and for rapid identification of pathogens in fluorescent antibody technique. It is also used as a reagent for microsequencing of proteins and peptides (HPLC). On celite it is used for the labeling of fibrinogen.
Solubility
Slightly soluble in DMSO, ethanol, methanol DMF and acetone
Notes
Incompatible with strong oxidizing agents, strong bases and amines.
RUO – Research Use Only
General References:
- Reagent for fluorescent labelling of proteins: Anal. Biochem., 20, 178 (1968).
- Guilini, C.; Baehr, C.; Schaeffer, E.; Gizzi, P.; Rufi, F.; Haiech, J.; Weiss, E.; Bonnet, D.; Galzi, J. New Fluorescein Precursors for Live Bacteria Detection. Anal. Chem. 2015, 87 (17), 8858-8866.
- Chen, Y.; Yang, C.; Li, T.; Zhang, M.; Liu, Y.; Gauthier, M. A.; Zhao, Y.; Wu, C. The Interplay of Disulfide Bonds, α-Helicity, and Hydrophobic Interactions Leads to Ultrahigh Proteolytic Stability of Peptides. Biomacromolecules 2015, 16 (8), 2347-2355.