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Citations & References (7)
Invitrogen™
LIVE BacLight™ Bacterial Gram Stain Kit, for microscopy & quantitative assays
LIVE BacLight™細菌グラム染色剤キットは、生細菌のグラムサインを識別するための、斬新なワンステップ蛍光アッセイです。グラム陰性菌とグラム陽性菌を、膜透過型のSYTO™ 9色素およびヨウ化ヘキシジウムで同時に染色すると、グラム陰性菌は緑色蛍光に、グラム陽性菌は赤色蛍光に発色します。詳細を見る
| 製品番号（カタログ番号） | 数量 |
|---|---|
| L7005 | 1 kit |
製品番号（カタログ番号） L7005
価格（JPY）お問い合わせください ›
130,100
Each
数量:
1 kit
LIVE BacLight™細菌グラム染色剤キットは、生細菌のグラムサインを識別するための、斬新なワンステップ蛍光アッセイです。グラム陰性菌とグラム陽性菌を、膜透過型のSYTO™ 9色素およびヨウ化ヘキシジウムで同時に染色すると、グラム陰性菌は緑色蛍光に、グラム陽性菌は赤色蛍光に発色します。
研究用にのみ使用できます。診断用には使用いただけません。
仕様
概要LIVE BacLight™ Bacterial Gram Stain Kit, for microscopy and quantitative assays
検出法蛍光
染色剤タイプその他の標識または色素
フォーマットチューブ、96ウェルプレート、スライド
数量1 kit
出荷条件室温
色Green, Red
Emission480 to 700
Excitation Wavelength Range470 nm
使用対象（アプリケーション）生存率アッセイ
使用対象 (装置)蛍光顕微鏡, フルオロメータ, フローサイトメーター, マイクロプレートリーダー
製品ラインBacLight
製品タイプBacterial Stain Kit
Unit SizeEach
組成および保存条件
フリーザー（-5℃～-30℃）に保存し、遮光してください。
よくあるご質問（FAQ）
What bacterial parameters can I look at by flow cytometry?
What is the excitation/emission maxima for SYTO 9 stain and hexidium iodide?
引用および参考文献 (7)
引用および参考文献
Abstract
Persistence and cell culturability of biocontrol strain Pseudomonas fluorescens CHA0 under plough pan conditions in soil and influence of the anaerobic regulator gene anr.
Journal:Environ Microbiol
PubMed ID:12558593
'Certain fluorescent pseudomonads can protect plants from soil-borne pathogens, and it is important to understand how these biocontrol agents survive in soil. The persistence of the biocontrol strain Pseudomonas fluorescens CHA0-Rif under plough pan conditions was assessed in non-sterile soil microcosms by counting total cells (immunofluorescence microscopy), intact cells (BacLight
Comparison of blue nucleic acid dyes for flow cytometric enumeration of bacteria in aquatic systems.
Journal:Appl Environ Microbiol
PubMed ID:9572943
'Seven blue nucleic acid dyes from Molecular Probes Inc. (SYTO-9, SYTO-11, SYTO-13, SYTO-16, SYTO-BC, SYBR-I and SYBR-II) were compared with the DAPI (4'',6-diamidino-2-phenylindole) method for flow cytometric enumeration of live and fixed bacteria in aquatic systems. It was shown that SYBR-II and SYTO-9 are the most appropriate dyes for bacterial
Determination of cell viability in single or mixed samples using capillary electrophoresis laser-induced fluorescence microfluidic systems.
Journal:Anal Chem
PubMed ID:11605830
The advent of high-efficiency microbial separations will have a profound effect on both chemistry and microbiology. For the first time, it appears that it may be possible to obtain qualitative and quantitative information on microbial systems with the accuracy, precision, speed, and throughput that currently is found for chemical systems.
Increased sensitivity of bacterial detection in cerebrospinal fluid by fluorescent staining on low-fluorescence membrane filters.
Journal:J Med Microbiol
PubMed ID:16091435
A membrane-filter-based, fluorescent Gram stain method for bacterial detection in cerebrospinal fluid samples was developed and evaluated as a rapid, sensitive alternative to standard Gram stain protocols. A recently developed, modified version of the aluminium oxide membrane Anopore with low-fluorescence optical properties showed superior performance in this application. Other aspects
Simultaneous fluorescent gram staining and activity assessment of activated sludge bacteria.
Journal:Appl Environ Microbiol
PubMed ID:12324319
Wastewater treatment is one of the most important commercial biotechnological processes, and yet the component bacterial populations and their associated metabolic activities are poorly understood. The novel fluorescent dye hexidium iodide allows assessment of Gram status by differential absorption through bacterial cell walls. Differentiation between gram-positive and gram-negative wastewater bacteria