LIVE/DEAD™ Yeast Viability Kit
LIVE/DEAD™ Yeast Viability Kit
Citations & References (12)
Invitrogen™

LIVE/DEAD™ Yeast Viability Kit

LIVE/DEAD酵母生存率キットは、酵母の生存率を調べるための新しい2色蛍光プローブであるFUN™ 1、真菌表面を標識する蛍光試薬であるCalcofluor™ White M2Rの組み合わせです。細胞内で黄色～緑色蛍光を示すFUN™ 1の染色は、原形質膜の完全性および真菌の代謝機能が存在する場合にのみ、赤色～橙色蛍光の液胞内構造に変換されます詳細を見る
テクニカルサポートオーダーサポート
製品番号（カタログ番号）数量
L70091 kit
製品番号（カタログ番号） L7009
価格（JPY）
130,100
Each
お問い合わせください ›
数量:
1 kit
LIVE/DEAD酵母生存率キットは、酵母の生存率を調べるための新しい2色蛍光プローブであるFUN™ 1、真菌表面を標識する蛍光試薬であるCalcofluor™ White M2Rの組み合わせです。細胞内で黄色～緑色蛍光を示すFUN™ 1の染色は、原形質膜の完全性および真菌の代謝機能が存在する場合にのみ、赤色～橙色蛍光の液胞内構造に変換されます。Calcofluor White M2Rは代謝の状態に関係なく、細胞壁キチンを青色蛍光で標識します。
研究用にのみ使用できます。診断用には使用いただけません。
仕様
細胞タイプ酵母細胞
概要LIVE/DEAD™ Yeast Viability Kit
検出法蛍光
染色剤タイプその他の標識または色素
フォーマットチューブ、96ウェルプレート、スライド
数量1 kit
出荷条件室温
色Yellow-Green, Red-Orange, Blue
Emission530/620, 500/550
Excitation Wavelength Range485, 450 nm
使用対象（アプリケーション）生存率アッセイ
使用対象 (装置)蛍光顕微鏡, フルオロメータ, フローサイトメーター, マイクロプレートリーダー
製品ラインLIVE/DEAD
製品タイプYeast Viability Kit
Unit SizeEach
組成および保存条件
フリーザー（-5℃～-30℃）に保存し、遮光してください。

よくあるご質問（FAQ）

Which species of yeast/fungi can I use with the LIVE/DEAD Yeast Viability Kit?

The LIVE/DEAD Yeast Viability Kit has been tested on the following fungi: S. cerevisiae (five different strains), Candida pseudotropicalis, Neurospora crassa and Aspergillus nidulans. A good correlation between the results obtained with the LIVE/DEAD Yeast Viability Kit and those obtained with standard plate counts was achieved with both S. cerevisiae and C. pseudotropicalis. Tests have been performed on both logarithmically growing cultures and cells that have undergone different forms of environmental stress

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

How do I prepare dead cell controls for LIVE/DEAD cell viability assays?

There are two easy options. One is to heat-inactivate the cells by placing at 60 degrees C for 20 minutes. The second is to subject the cells to 70% ethanol. Alcohol-fixed cells can be stored indefinitely in the freezer until use, potentially up to several years.

Centrifuge cells, pellet, and remove supernatant.
Fix cells: Add 10 mL ice cold 70% ETOH to a 15 mL tube containing the cell pellet, adding dropwise at first while vortexing, mix well.
Store in freezer until use.
When ready to use, wash twice and resuspend in buffer of choice.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Can FUN 1 stained cells be examined by flow cytometry?

Yes. Use a 488 nm laser line and standard FITC and PE channels for two-color detection of green (dead/metabolically inactive cells) and red (live, metabolically active cells) emission.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Live, metabolically active fungi transport FUN 1 into vacuoles to give a red-shifted fluorescence versus green/yellow fluorescence in the nucleus and cytoplasm of dead or metabolically-inactive cells. Is this a reliable indicator of fungal viability?

No. FUN 1 accumulates into vacuoles by an unknown transport pathway, but any mutants/ recombinant cells or experimental treatments that result in a deficiency or block in vesicle-mediate transport into vacuoles may result in cells that do not have red vacuoles, even though the cells are live and metabolically active. For more information see J Microbiol Methods 78:208 (2009).

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

引用および参考文献 (12)

引用および参考文献
Abstract
In vitro growth and analysis of Candida biofilms.
Authors:Chandra J, Mukherjee PK, Ghannoum MA,
Journal:Nat Protoc
PubMed ID:19180075
'Evaluation of fungal biofilm formation can be performed using several techniques. In this protocol, we describe methods used to form Candida biofilms on three different medical device substrates (denture strips, catheter disks and contact lenses) to quantify them and to evaluate their architecture and drug susceptibility. Biofilm formation involves adhesion ... More
Mechanistic aspects in the generation of apparent ultrahigh efficiencies for colloidal (microbial) electrokinetic separations.
Authors:Armstrong DW, Girod M, He L, Rodriguez MA, Wei W, Zheng J, Yeung ES
Journal:Anal Chem
PubMed ID:12433083
'Under specific experimental conditions, the electrokinetic separation of certain microorganisms can produce peaks of very high apparent efficiencies (approximately 10(6)-10(10) theoretical plates/m). This is unusual in that no deliberate focusing mechanism was employed. To investigate this process further, the separation was monitored in real time using a charge-coupled device (CCD) ... More
Pleiotropic effects of HIV-1 protein R (Vpr) on morphogenesis and cell survival in fission yeast and antagonism by pentoxifylline.
Authors:Zhao Y, Yu M, Chen M, Elder RT, Yamamoto A, Cao J
Journal:Virology
PubMed ID:9657945
'Expression of HIV-1 Vpr causes cell cycle G2 arrest, change in cell shape, and cell death over a large evolutionary distance ranging from human to yeast cells. As a step toward understanding these highly conserved Vpr functions, we have examined the effect of Vpr on cytoskeletal elements and the viability ... More
Mechanism of fluconazole resistance in Candida albicans biofilms: phase-specific role of efflux pumps and membrane sterols.
Authors:Mukherjee PK, Chandra J, Kuhn DM, Ghannoum MA
Journal:Infect Immun
PubMed ID:12874310
'Candida albicans biofilms are formed through three distinct developmental phases and are associated with high fluconazole (FLU) resistance. In the present study, we used a set of isogenic Candida strains lacking one or more of the drug efflux pumps Cdr1p, Cdr2p, and Mdr1p to determine their role in FLU resistance ... More
Adaptation of FUN-1 and Calcofluor white stains to assess the ability of viable and nonviable yeast to adhere to and be internalized by cultured mammalian cells.
Authors:Henry-Stanley MJ, Garni RM, Wells CL,
Journal:J Microbiol Methods
PubMed ID:15369865
'The FUN-1 and Calcofluor white stains can be used in concert to assess the ability of viable and nonviable yeast to adhere to, and be internalized by, host mammalian cells in vitro. With this method, only extracellular yeast stain with Calcofluor, dead yeast cells have diffuse cytoplasmic yellow-green fluorescence, and ... More
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