LIVE/DEAD™ Cell-Mediated Cytotoxicity Kit, for animal cells

Citations & References (14)

Invitrogen™

LIVE/DEAD™ Cell-Mediated Cytotoxicity Kit, for animal cells

LIVE/DEAD™細胞媒介性細胞毒性キットは、ナチュラルキラー（NK）細胞介在性、リンフォカイン活性化キラー（LAK）細胞介在性、およびT細胞介在性の細胞毒性を測定します。まず、標的細胞を緑色蛍光の膜染色剤DiOC18でプレインキュベートしておき詳細を見る

製品番号（カタログ番号）	数量
L7010	1 kit

製品番号（カタログ番号） L7010

価格（JPY）

71,200

お問い合わせください ›

1 kit

LIVE/DEAD™細胞媒介性細胞毒性キットは、ナチュラルキラー（NK）細胞介在性、リンフォカイン活性化キラー（LAK）細胞介在性、およびT細胞介在性の細胞毒性を測定します。まず、標的細胞を緑色蛍光の膜染色剤DiOC₁₈でプレインキュベートしておき、その後、膜非透過性の赤色蛍光染色剤であるヨウ化プロピジウムの存在下でエフェクター細胞と混合します。標的生細胞と標的死細胞は、緑色蛍光膜染色を保持します。膜が損なわれた標的細胞とエフェクター細胞は、赤色蛍光の核酸染色を示します。生きているエフェクター細胞は非蛍光性です。

研究用にのみ使用できます。診断用には使用いただけません。

細胞タイプ哺乳類細胞、真核細胞

概要LIVE/DEAD™ Cell-Mediated Cytotoxicity Kit, for animal cells

検出法蛍光

染色剤タイプその他の標識または色素

フォーマットチューブ、スライド

数量1 kit

出荷条件室温

色Green, Red

Emission501/617

Excitation Wavelength Range484, 536 nm

使用対象（アプリケーション）生存率アッセイ

使用対象 (装置)蛍光顕微鏡, フローサイトメーター

製品ラインLIVE/DEAD

製品タイプCell-Mediated Cytotoxicity Kit

Unit SizeEach

組成および保存条件

冷蔵庫（2℃～8℃）に保存し、遮光してください。

よくあるご質問（FAQ）

Which optical filter sets are compatible with the LIVE/DEAD Cell-Mediated Cytotoxicity Kit?

The fluorescence from both live and dead bacteria stained with the LIVE/DEAD Cell-Mediated Cytotoxicity Kit may be viewed simultaneously with any standard fluorescein long-pass filter set. Alternatively, the fluorescent live and dead cells may be viewed separately with fluorescein and either rhodamine or Texas Red bandpass filter sets. A summary of the fluorescence microscope filter sets recommended for use with the LIVE/ DEAD Cell-Mediated Cytotoxicity Kit is shown in Table 1 on Page 2 in the manual (https://tools.thermofisher.com/content/sfs/manuals/mp07010.pdf).

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

引用および参考文献 (14)

引用および参考文献

Abstract

Evaluation of human mast cell-mediated cytotoxicity by DIOC18 target cell labeling in flow cytometry.

Authors:Ozdemir O,

Journal:J Immunol Methods

PubMed ID:17188705

'(51)Cr release assay (CRA) is still the standard method to study mast cell (MC)-mediated cytotoxicity in vitro. Non-radioactive methods e.g. MTT, Hoechst 22147 staining, have also been used. Though CRA has the benefit of being reproducible, it has several drawbacks e.g. spontaneous release and radioactivity. The basic strategy of this ... More

Development of a lung slice preparation for recording ion channel activity in alveolar epithelial type I cells.

Authors:Bourke S, Mason HS, Borok Z, Kim KJ, Crandall ED, Kemp PJ,

Journal:Respir Res

PubMed ID:15857506

'Lung fluid balance in the healthy lung is dependent upon finely regulated vectorial transport of ions across the alveolar epithelium. Classically, the cellular locus of the major ion transport processes has been widely accepted to be the alveolar type II cell. Although evidence is now emerging to suggest that the ... More

Direct visualisation and quantification of cellular cytotoxicity using two colour flourescence.

Authors:Kroesen BJ, Mesander G, ter Haar JG, The TH, de Leij L

Journal:J Immunol Methods

PubMed ID:1431162

A fluorescence method is described for the evaluation of cell death induced by cellular cytolytic activity. A green fluorescent membrane dye, D275, was used to label various target cell lines and propidium iodide (PI) uptake was used to assay cell death. Natural killer (NK), lymphokine activated killer (LAK) as well ... More

Rapid flow cytometric assay for the assessment of natural killer cell activity.

Authors:Chang L, Gusewitch GA, Chritton DB, Folz JC, Lebeck LK, Nehlsen-Cannarella SL

Journal:J Immunol Methods

PubMed ID:8228287

A new assay using flow cytometry has been established to assess natural killer (NK) lytic activity with common bench top instrumentation. This assay uses a cyanine membrane dye to stain live K562 target cells and an iodide nuclear dye to evaluate dead cells, and provides a method of reliably separating ... More

Human Vγ2Vδ2 T cells limit breast cancer growth by modulating cell survival-, apoptosis-related molecules and microenvironment in tumors.

Authors:

Journal:Int J Cancer

PubMed ID:23595559

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