LIVE/DEAD™ Sperm Viability Kit
LIVE/DEAD™ Sperm Viability Kit
Citations & References (69)
Invitrogen™

LIVE/DEAD™ Sperm Viability Kit

LIVE/DEAD Sperm Viability Kitは、蛍光顕微鏡またはフローサイトメトリーによって精子の生存率および受精可能性を分析するための、蛍光ベースの新しいアッセイを提供します。膜透過性の核酸染色剤であるSYBR™ 14は、精子生細胞を緑色蛍光で標識します。一方詳細を見る
テクニカルサポートオーダーサポート
製品番号（カタログ番号）数量
L70111 kit
製品番号（カタログ番号） L7011
価格（JPY）
130,100
Each
お問い合わせください ›
数量:
1 kit
LIVE/DEAD Sperm Viability Kitは、蛍光顕微鏡またはフローサイトメトリーによって精子の生存率および受精可能性を分析するための、蛍光ベースの新しいアッセイを提供します。膜透過性の核酸染色剤であるSYBR™ 14は、精子生細胞を緑色蛍光で標識します。一方、膜非透過性のヨウ化プロピジウムは、膜の損傷した精子細胞の核酸を赤色蛍光で標識します。
研究用にのみ使用できます。診断用には使用いただけません。
仕様
細胞タイプ真核細胞、精子細胞
概要LIVE/DEAD™ Sperm Viability Kit
検出法蛍光
染色剤タイプその他の標識または色素
フォーマットチューブ、スライド
数量1 kit
出荷条件室温
色Green, Red
Emission516/617
使用対象（アプリケーション）生存率アッセイ
使用対象 (装置)蛍光顕微鏡, フローサイトメーター
製品ラインLIVE/DEAD
製品タイプLIVE/DEAD Sperm Viability Kit
Unit SizeEach
組成および保存条件
フリーザー（-5℃～-30℃）に保存し、遮光してください。

よくあるご質問（FAQ）

What is the fluorescence emission maxima of SYBR 14 dye and propidium iodide?

When bound to DNA, the fluorescence emission maxima of these dyes are 516 nm and 617 nm, respectively. The spectra are available in Figure 1 of the Product Manual.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

How do I prepare dead cell controls for LIVE/DEAD cell viability assays?

There are two easy options. One is to heat-inactivate the cells by placing at 60 degrees C for 20 minutes. The second is to subject the cells to 70% ethanol. Alcohol-fixed cells can be stored indefinitely in the freezer until use, potentially up to several years.

Centrifuge cells, pellet, and remove supernatant.
Fix cells: Add 10 mL ice cold 70% ETOH to a 15 mL tube containing the cell pellet, adding dropwise at first while vortexing, mix well.
Store in freezer until use.
When ready to use, wash twice and resuspend in buffer of choice.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

引用および参考文献 (69)

引用および参考文献
Abstract
Seminal plasma addition attenuates the dilution effect in bovine sperm.
Authors:Garner DL,Thomas CA,Gravance CG,Marshall CE,DeJarnette JM,Allen CH
Journal:Theriogenology
PubMed ID:11467516
Dilution of semen to low cell numbers/dose can result in a bull-dependent reduction in the post-thaw viability of cryopreserved bovine spermatozoa. It is possible that essential seminal plasma components are lacking at the greater dilution rates, thereby contributing to the deleterious effects of semen dilution. Ejaculates of 6 Holstein bulls ... More
Identification of amplified restriction fragment length polymorphism markers linked to genes controlling boar sperm viability following cryopreservation.
Authors:Thurston LM, Siggins K, Mileham AJ, Watson PF, Holt WV
Journal:Biol Reprod
PubMed ID:11870056
This study investigated two hypotheses: 1) that consistent between-boar variation in frozen semen quality exists and is genetically determined, and 2) molecular markers linked to genes controlling semen freezability can be identified using amplified restriction fragment length polymorphism (AFLP) technology. Five ejaculates were collected from each of 129 boars. Semen ... More
Comparison of assessment of fowl sperm viability by eosin-nigrosin and dual fluorescence (SYBR-14/PI).
Authors:Chalah T, Brillard JP
Journal:Theriogenology
PubMed ID:10732141
'The kinetics of fowl sperm viability/mortality following short-term and long-term in vitro storage were studied using 2 different staining methods: eosin/nigrosin (observed under light microscopy) and SYBR-14/PI (dual fluorescence). Based on data obtained at 0, 30 min and at 2, 4 and 24 h (T0, T30, T2, T4, and T24) ... More
Seasonal changes of semen quality and freezability in Franches-Montagnes stallions.
Authors:Janett F, Thun R, Bettschen S, Burger D, Hassig M
Journal:Anim Reprod Sci
PubMed ID:12695055
'The objective of this study was to investigate seasonal changes of semen quality parameters in Franches-Montagnes stallions and to compare the freezability of ejaculates collected in autumn and winter. Experiments were performed using 15 stallions from the National Stud Farm in Avenches (Switzerland). Ejaculates were collected and evaluated every month ... More
Flow cytometric assessment of allopurinol susceptibility in Leishmania infantum promastigote.
Authors:Kamau SW, Hurtado M, Müller-Doblies UU, Grimm F, Nunez R
Journal:Cytometry
PubMed ID:10918286
'BACKGROUND: Leishmaniasis is a major tropical and subtropical parasitic disease. Sodium stibogluconate, N-methyl -D-glucamine antimoniate, amphotericin B, pentamidine, and ketoconazole are drugs used to treat this disease. Some of these drugs cause severe adverse side effects and treatment failures are common. Allopurinol, a purine analog, has been used to treat ... More
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