Novex™ Sharp Pre-stained Protein Standard

製品番号（カタログ番号）	数量
LC5800	2x250 μL

製品番号（カタログ番号） LC5800

価格（JPY）

34,000

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2x250 μL

一括またはカスタム形式をリクエストする

Novex Sharp事前染色済みタンパク質標準液は、SDS-PAGEおよびウェスタンブロッティング中の幅広い分子量タンパク質の正確かつ簡単で便利な分子量推定を目的として設計されています。この標準液は3.5～260 kDaの範囲の12色のバンドで構成されています。また、ゲルに直接ロードでき、使用前にサンプルバッファーを加熱、減少、または追加する必要はありません。

その他のあらゆるタンパク質標準物質およびラダーの比較と閲覧›

アプリケーション
• SDSポリアクリルアミドゲル電気泳動中のタンパク質移動のモニタリング
• ウェスタンブロッティング後の膜へのタンパク質転写のモニタリング
• SDS-PAGEゲルおよびウェスタンブロット上のタンパク質のサイジング

研究用にのみ使用できます。診断用には使用いただけません。

検出法比色法

ゲル適合性Bolt™ Bis-Tris Plusゲル、Novex™ Tricineゲル、Novex™ Tris-Glycineゲル、NuPAGE™ Bis-Trisゲル

分子量260、160、110、80、60、50、40、30、20、15、10、3.5 kDa

製品ラインNovex

製品タイプタンパク質標準液

数量2x250 μL

ロード可能状態可

出荷条件氷水またはドライアイスでの出荷が承認されています

染色タイプ3色：ピンク、黄、青

システムタイプウェスタンブロッティング、SDS-PAGE

Number of Markers12

サイズ範囲3.5～260 kDa

Unit SizeEach

組成および保存条件

Novex™ Sharp事前染色済みタンパク質標準液は、すぐに使用可能な標準混合物として2x250 μL(ぞれぞれ10 μLの合計50のアプリケーション)が提供されます。Novex™ Sharp事前染色済み標準液ローディングバッファーは、65 mM Tris pH 6.5、30%グリセロール、2% SDS、および2.5 mM ETDAで構成されています。-20°Cで保存

よくあるご質問（FAQ）

Why can I not see the 3.5 kDa band of the Invitrogen Sharp Pre-Stained Standard on my NuPAGE gels?

The 3.5 kDa band is visible only on NuPAGE gels with 1X MES SDS running buffer.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

What are the different molecular weight estimations for the Invitrogen Sharp Pre-Stained standard on the different Invitrogen gel types?

Invitrogen Sharp Pre-Stained Standard molecular weight estimations are the same in NuPAGE Bis-Tris, NuPAGE Tris-Acetate, Invitrogen Tris-Glycine, and Tricine Gels. The appearance/migration of the protein bands may differ depending upon the gel type and running conditions

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

What are the recommended protocols for preparing Invitrogen protein standards to load on a gel?

Invitrogen protein standards do not require any additional preparation. Thaw protein standards at room temperature, vortex to mix well, and load.

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

I used one of your pre-stained protein standards for a western transfer and I noticed that the intensity of the band faded from the membrane during the transfer process. Why is this?

The fading is most likely due to detergent in the western blocking/washing solutions that can remove some of the proteins from the membrane. The dye itself will not wash off of the proteins because it is covalently bound. We have found that smaller pore size membranes retain the proteins better during blocking and wash procedures, and hence recommend use of 0.2 µm instead of 0.45 µm membranes for best resolution and protein retention. After transfer, it is a good idea to circle the pre-stained bands with a pencil on the membrane, so band positions can be identified after blocking and processing.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

I used one of your protein standards for a western transfer and noticed that some of the lower-molecular weight protein bands passed through the membrane. How can I resolve this issue?

- Decrease voltage, current or length of transfer time
- Make sure that the methanol concentration in the transfer buffer is proper; use a methanol concentration of 10-20% methanol removes the SDS from SDS-protein complexes and improves the binding of protein to the membrane.
- Make sure that the SDS concentration (if added) in the transfer buffer is proper, don't use more than 0.02-0.04% SDS. Using too much SDS can prevent binding of proteins to the membrane.
- Check the pore size of the membrane and the size of the target protein. Proteins smaller than 10 kDa will easily pass through a 0.45 µm pore size membrane. If proteins smaller than 10 kDa are of interest, it would be better to use a 0.2 µm pore size membrane.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

View all Novex™ Sharp Pre-stained Protein Standard FAQs