Colloidal Blue Staining Kit

Citations & References (4)

Invitrogen™

Colloidal Blue Staining Kit

製品番号（カタログ番号）	数量
LC6025	1 kit

製品番号（カタログ番号） LC6025

価格（JPY）

52,200

お問い合わせください ›

1 kit

一括またはカスタム形式をリクエストする

コロイドブルー染色キットを使用することで、最小限の労力で1Dまたは2D PAGEゲル内のタンパク質をナノグラムレベルで検出できます。脱染に必要なのは水のみです。コロイドブルー染色はコロイド化学を使用しており、溶液中の遊離色素を減らし、タンパク質と色素の結合比を改善します。サンプルは3時間以内に強く染色され、可視化されます。バックグラウンド染色は、水で一晩脱色することでほぼ除去されます。このキットは、簡単な溶液調製が1つ必要です。染色手順にはメタノールが必要ですが、キットには含まれていません。

すべてのCoomassie染色を比較›

研究用にのみ使用できます。診断用には使用いただけません。

検出位置ゲル内検出

検出法比色法

製品タイプタンパク質ゲル染色キット

数量1 kit

品質保持期間6カ月

出荷条件室温

標的分子タンパク質

標識または色素Coomassie

Unit SizeEach

組成および保存条件

コロイドブルー染色キットには、25のミニゲルを染色するのに十分な染色液Aおよび染色液Bが付属しています。キットは室温で保管してください。本キットは、製品ドキュメントに特に記載されていない限り、適切に保管されている場合は6カ月間保証されます。

よくあるご質問（FAQ）

カスタムオリゴ（TaqMan®プローブ・プライマー）を購入しました。TaqMan® Gene Expression Assayのように20xのワーキングストックを作成したいのですが、どのように調整したらよいでしょうか。

大部分のデザイン済みTaqMan®アッセイは20倍濃度で提供されています。各18uMのプライマーと5uMのプローブが含まれており、最終的に1xの終濃度が各プライマー900nMとプローブ250nM（Gene Expression Assays用）もしくは200nM（SNP Genotyping assay用）になります。カスタムオリゴ合成で20xワーキングストックを作成するためには、以下の様に希釈する必要があります。遺伝子発現解析用の20xストックを作るには、まず100uMのForwardプライマー、ReverseプライマーおよびTaqMan®プローブを作成します。次に18ulの各プライマーと5ulのTaqMan®プローブを混ぜて41ulの混合液を作ります。そこへ59ulの1xTEを加え100ulの遺伝子発現解析用20x ストックが完成します。

Can I use the Colloidal Blue Staining Kit (Cat. No. LC6025) for membrane staining to detect proteins that are transferred to a membrane?

It is not recommended because the background will be too high. Better alternatives include: 1) Invitrogen Reversible Membrane Protein Stain Kit (Cat. No. IB7710).

2) Coomassie (non-colloidal) staining: stain in 0.1% Coomassie Blue R-250 in 50% methanol for 5 min and destain with several changes of 50% methanol and 10% acetic acid. Rinse with several changes of water, air dry and store for up to 12 months at -20°C. Sensitivity is approximately at the 50-100 ng level.

3) Use SimplyBlue SafeStain (Cat. No. LC6060). The SimplyBlue SafeStain manual has the protocol for staining PVDF membranes, but it is not recommended for nitrocellulose because of high background.

4) Amido Black: same as Coomassie but less sensitive.

5) Ponceau S: same as Coomassie but less sensitive.

6) UV transillumination: place membrane on filter paper after blot is finished and allow to dry at room temperature for about 10 min. Rewet in 20% methanol and view the blot in front of white light while it is still wet; the bands will look more translucent than the membrane. If the bands disappear as the membranes dries, rewet again.

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

Why is Coomassie G-250 used as the tracking dye in NuPAGE LDS Sample Buffer instead of bromophenol blue?

Coomassie G-250 will give a sharp dye front with both NuPAGE MES and MOPS Running Buffers and is therefore used as the tracking dye in the NuPAGE LDS Sample Buffer.

Bromophenol blue runs more slowly than some peptides with the NuPAGE MES Running Buffer system.

Coomassie G-250 migrates much closer to the moving ion front than bromophenol blue, ensuring that small peptides will not be run too far (e.g., off the gel).

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

Is the SimplyBlue SafeStain the appropriate stain for quantitation by densitometry?

The Colloidal Blue Staining Kit (Cat. No. LC6025) is best for quantitation by densitometry. You can also use SimplyBlue SafeStain for this application.

The great advantage of SimplyBlue SafeStain is that it is very easy to use and safe.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

The sensitivity of my SimplyBlue SafeStain seems to be decreasing over time. Why is this?

Check the cap on the bottle. If the bottles are not tightly sealed, the alcohol can evaporate from the stain causing substantial decrease in stain sensitivity.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

View all Colloidal Blue Staining Kit FAQs

引用および参考文献 (4)

引用および参考文献

Abstract

SALSA, a variant of yeast SAGA, contains truncated Spt7, which correlates with activated transcription.

Authors: Sterner David E; Belotserkovskaya Rimma; Berger Shelley L;

Journal:Proc Natl Acad Sci U S A

PubMed ID:12186975

'Spt-Ada-Gcn5 acetyltransferase (SAGA) is a previously described histone acetyltransferase/transcriptional coactivator complex in yeast. At promoters of certain genes (HIS3 and TRP3), SAGA has an inhibitory function involving a nonproductive TATA-binding protein interaction mediated by the Spt3 and Spt8 subunits. Related to this, Spt8-less SAGA is a major form of the ... More

Chromatin deacetylation by an ATP-dependent nucleosome remodelling complex.

Authors:Tong JK, Hassig CA, Schnitzler GR, Kingston RE, Schreiber SL

Journal:Nature

PubMed ID:9804427

'The dynamic assembly and remodelling of eukaryotic chromosomes facilitate fundamental cellular processes such as DNA replication and gene transcription. The repeating unit of eukaryotic chromosomes is the nucleosome core, consisting of DNA wound about a defined octamer of histone proteins. Two enzymatic processes that regulate transcription by targeting elements of ... More

A family of chromatin remodeling factors related to Williams syndrome transcription factor

Authors:Bochar DA, Savard J, Wang W, Lafleur DW, Moore P, Cote J, Shiekhattar R

Journal:Proc Natl Acad Sci U S A

PubMed ID:10655480

Chromatin remodeling complexes have been implicated in the disruption or reformation of nucleosomal arrays resulting in modulation of transcription, DNA replication, and DNA repair. Here we report the isolation of WCRF, a new chromatin-remodeling complex from HeLa cells. WCRF is composed of two subunits, WCRF135, the human homolog of Drosophila ... More

A method to identify serine kinase substrates. Akt phosphorylates a novel adipocyte protein with a Rab GTPase-activating protein (GAP) domain.

Authors: Kane Susan; Sano Hiroyuki; Liu Simon C H; Asara John M; Lane William S; Garner Charles C; Lienhard Gustav E;

Journal:J Biol Chem

PubMed ID:11994271

This study describes a method for the identification of the substrates of specific serine kinases. An antibody specific for the phosphomotif generated by the kinase is used to isolate phosphorylated substrates by immunoprecipitation, and the isolated proteins are identified by tandem mass spectrometry of peptides. This method was applied to ... More