SilverQuest™ Silver Staining Kit

Citations & References (5)

Invitrogen™

SilverQuest™ Silver Staining Kit

SilverQuestシルバー染色キットは、ポリアクリルアミドゲルでタンパク質や DNA を銀染色するための迅速かつ簡単な方法を提供します。質量分析に適合する高感度な銀染色を提供するように特別に設計されています。すべてのシルバー染色を比較›このキットの特長：• 互換性—質量分析と互換性のある染色• プロトコル—タンパク質のバンド（0.3 ngのタンパク質詳細を見る

製品番号（カタログ番号）	数量
LC6070	1 kit

製品番号（カタログ番号） LC6070

価格（JPY）

60,400

お問い合わせください ›

1 kit

一括またはカスタム形式をリクエストする

SilverQuestシルバー染色キットは、ポリアクリルアミドゲルでタンパク質や DNA を銀染色するための迅速かつ簡単な方法を提供します。質量分析に適合する高感度な銀染色を提供するように特別に設計されています。

すべてのシルバー染色を比較›

このキットの特長：
• 互換性—質量分析と互換性のある染色
• プロトコル—タンパク質のバンド（0.3 ngのタンパク質）は1時間以内に可視化することができ、さらにスピードを上げるためにはマイクロ波処理を30分程度で完了させることができます
• カラーコード化されたソリューション—プロトコルを簡単に染色できるように色分けされています

質量分析と互換性のある試薬
SilverQuestシルバー染色キットは、タンパク質検出を質量分析と互換性があるように特別に設計されています。SilverQuest試薬セットには、グルタルアルデヒドやホルムアルデヒドを含まない感作剤が含まれているため、リジン側鎖とアルギニン側鎖はそのままになります。さらに、SilverQuest脱染色液は、ポリアクリルアミドゲルのタンパク質バンドから銀を効果的に除去します。ペプチドは、ゲル内トリプシン消化およびゲル抽出の効率的なターゲットとして維持され、質量分析中のカバレッジが向上します。

研究用にのみ使用できます。診断用には使用いただけません。

検出位置ゲル内検出

検出法比色法

数量1 kit

品質保持期間6カ月

標的分子タンパク質

標識または色素SilverQuest シルバー染色剤

製品ラインSilverQuest

製品タイプタンパク質ゲル染色キット

Unit SizeEach

組成および保存条件

このキットには、25のミニゲルを染色するのに十分な試薬が含まれています。

内容：
感作剤250 mL
染色液25 mL
現像液250 mL
現像液エンハンサー2 mL
停止液250 mL
デステイナーA 60 mL
デステイナーB 60 mL

キットは室温（15～30℃）で保存してください。6ヵ月安定です。

よくあるご質問（FAQ）

How much protein is required for mass spectrometry analysis by MALDI-TOF?

In general, the required amount needed to identify a gel-separated protein by enzymatic digestion (usually Trypsin) is about 2 pmole (equivalent to approximately 0.12 µg). Our SilverQuest Silver staining kit is mass spectrometry compatible.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

If you need to stop in the middle of the SilverQuest or SilverXpress silver staining procedure, at what point should this be done?

The sensitizing step is the optimal point to stop the procedure. The gel can be left overnight in the Sensitizing Solution. Although some other kits recommend leaving gels in the fix step, we have found that overnight fixation diminishes stain performances.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

What is the sensitivity of Coomassie staining and silver staining?

Coomassie sensitivity: 50-500 ng protein per band
Silver-staining sensitivity: 1-5 ng protein per band
In general, silver staining is 10-100 times more sensitive than Coomassie staining.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

I used the SilverQuest Silver Staining kit and I see negative staining (a dark halo enclosing a pale area). Can you please help me troubleshoot?

This is usually due to overloading of the protein sample. We recommend decreasing the protein load per band. For an unknown protein, a serial dilution may be necessary to determine the best amount to load for a particular protein.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

I used the SilverQuest Silver Staining kit and it took a longer time for band development resulting in a dark background. What happened?

Here are possible causes and solutions:

- Low protein load. Increase the amount of protein load. Be sure to have at least 1-5 ng protein on the gel.
- Some proteins may need longer fixing time. Increase the time for fixing the gel to 2 hours or overnight.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

View all SilverQuest™ Silver Staining Kit FAQs

引用および参考文献 (5)

引用および参考文献

Abstract

The Wnt signaling inhibitor dickkopf-1 is required for reentry into the cell cycle of human adult stem cells from bone marrow.

Authors:Gregory CA, Singh H, Perry AS, Prockop DJ,

Journal:J Biol Chem

PubMed ID:12740383

'Adult human mesenchymal stem cells from bone marrow stroma (hMSCs) differentiate into numerous mesenchymal tissue lineages and are attractive candidates for cell and gene therapy. When early passage hMSCs are plated or replated at low density, the cultures display a lag phase of 3-5 days, a phase of rapid exponential ... More

Physical and functional interaction of the active zone proteins, CAST, RIM1, and Bassoon, in neurotransmitter release.

Authors:Takao-Rikitsu E, Mochida S, Inoue E, Deguchi-Tawarada M, Inoue M, Ohtsuka T, Takai Y,

Journal:J Cell Biol

PubMed ID:14734538

'We have recently isolated a novel cytomatrix at the active zone (CAZ)-associated protein, CAST, and found it directly binds another CAZ protein RIM1 and indirectly binds Munc13-1 through RIM1; RIM1 and Munc13-1 directly bind to each other and are implicated in priming of synaptic vesicles. Here, we show that all ... More

The R116C Mutation in alpha A-crystallin Diminishes Its Protective Ability against Stress-induced Lens Epithelial Cell Apoptosis.

Authors: Andley Usha P; Patel Harendra C; Xi Jing-Hua;

Journal:J Biol Chem

PubMed ID:11756414

'alphaA-crystallin is a small heat-shock protein expressed preferentially in the lens and is detected during the early stages of lens development. Recent work indicates that the expression of alphaA-crystallin enhances lens epithelial cell growth and resistance to stress conditions. Mutation of the arginine 116 residue to cysteine (R116C) in alphaA-crystallin ... More

The formation of highly soluble oligomers of alpha-synuclein is regulated by fatty acids and enhanced in Parkinson's disease.

Authors:Sharon R, Bar-Joseph I, Frosch MP, Walsh DM, Hamilton JA, Selkoe DJ,

Journal:Neuron

PubMed ID:12597857

Accumulation of misfolded proteins as insoluble aggregates occurs in several neurodegenerative diseases. In Parkinson's disease (PD) and dementia with Lewy bodies (DLB), alpha-synuclein (alpha S) accumulates in insoluble inclusions. To identify soluble alpha S oligomers that precede insoluble aggregates, we probed the cytosols of mesencephalic neuronal (MES) cells, normal and ... More

The semaphorin receptor plexin-B1 signals through a direct interaction with the Rho-specific nucleotide exchange factor, LARG.

Authors: Aurandt Jennifer; Vikis Haris G; Gutkind J Silvio; Ahn Natalie; Guan Kun-Liang;

Journal:Proc Natl Acad Sci U S A

PubMed ID:12196628

Semaphorins are axon guidance molecules that signal through the plexin family of receptors. Semaphorins also play a role in other processes such as immune regulation and tumorigenesis. However, the molecular signaling mechanisms downstream of plexin receptors have not been elucidated. Semaphorin 4D is the ligand for the plexin-B1 receptor and ... More