SilverXpress™ Silver Staining Kit

製品番号（カタログ番号）	数量
LC6100	1 kit

製品番号（カタログ番号） LC6100

価格（JPY）

54,000

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1 kit

一括またはカスタム形式をリクエストする

SilverXpress銀染色キットは、バックグラウンドを最小限に抑えながらナノグラムレベルの感度を実現する、高感度な銀染色キットです。プロトコルは簡単に従うことができるため、標準的なCoomassieよりかかる時間が短く、わずか1時間程度で完了します。

すべての銀染色を比較›

SilverXpress銀染色キットは、アンモニア銀化学とグルタルアルデヒド感作を使用して高感度の銀染色を行い、標準的なCoomassieやコロイドブルー技術よりはるかに低いレベルのタンパク質を検出できます。バックグラウンドが透明なため、サンプルの識別が明確になり、論文使用レベルの質のゲルが得られます。

DNA染色
SilverXpressキットは、エチジウムブロマイドでは検出できないDNAのナノグラム濃度の検出にも使用できます。エチジウムブロマイドの5倍の感度を有し、わずか<0.5 ng未満の50 bp DNAを検出します。

研究用にのみ使用できます。診断用には使用いただけません。

検出位置ゲル内検出

検出法比色法

製品ラインSilverXpress

製品タイプタンパク質ゲル染色キット

数量1 kit

品質保持期間6カ月

出荷条件室温

標的分子タンパク質

標識または色素SilverXpress銀染色

Unit SizeEach

組成および保存条件

このキットには、25ミニゲルを染色するのに十分な試薬が含まれています

内容：
125 mL増感剤
125 mL染色液A
125 mL染色液B
125 mL現像剤
125ストッパー溶液

キットは2℃～8℃で保管してください。これらの条件下で保管した場合は6カ月間安定します。

よくあるご質問（FAQ）

Can I stain my TBE gel? How?

Yes, you can stain your TBE gels with ethidium bromide, SYBR Green I, SYBR Green II, and the SilverXpress Silver Staining Kit. For ethidium bromide staining, soak the gel in a 2 µg/mL solution of ethidium bromide in ultrapure water for 20 minutes. Destain by rinsing with three successive 10-minute rinses of ultrapure water. Visualize bands under UV light.

If you need to stop in the middle of the SilverQuest or SilverXpress silver staining procedure, at what point should this be done?

The sensitizing step is the optimal point to stop the procedure. The gel can be left overnight in the Sensitizing Solution. Although some other kits recommend leaving gels in the fix step, we have found that overnight fixation diminishes stain performances.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

What is the sensitivity of Coomassie staining and silver staining?

Coomassie sensitivity: 50-500 ng protein per band
Silver-staining sensitivity: 1-5 ng protein per band
In general, silver staining is 10-100 times more sensitive than Coomassie staining.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

I used the SilverXpress Silver Staining kit and my stained gels are too dark. What went wrong?

Here are possible causes and solutions:

- Stopper not added to the gel at the appropriate time. Be sure to add the stopper slightly before the desired stain intensity is reached.
- Protein is overloaded. Decrease the protein load on the gel.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

I used the SilverXpress Silver Staining kit and I see negative staining (a dark halo enclosing a pale area). Can you please help me troubleshoot?

This is usually due to overloading of the protein sample. We recommend decreasing the protein load per band. For an unknown protein, a serial dilution may be necessary to determine the best amount to load for a particular protein.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

View all SilverXpress™ Silver Staining Kit FAQs

引用および参考文献 (1)

引用および参考文献

Abstract

DNA binding site selection of dimeric and tetrameric Stat5 proteins reveals a large repertoire of divergent tetrameric Stat5a binding sites.

Authors:Soldaini E, John S, Moro S, Bollenbacher J, Schindler U, Leonard WJ

Journal:Mol Cell Biol

PubMed ID:10594041

We have defined the optimal binding sites for Stat5a and Stat5b homodimers and found that they share similar core TTC(T/C)N(G/A)GAA interferon gamma-activated sequence (GAS) motifs. Stat5a tetramers can bind to tandemly linked GAS motifs, but the binding site selection revealed that tetrameric binding also can be seen with a wide ... More