PEN Membrane Frame Slides for Live Cell Microdissection

The Arcturus HistoGene LCM Frozen Section Staining Kit (Cat. No. KIT0401) comes complete with all the reagents and supplies needed for preparing frozen tissue sections for laser capture microdissection (LCM). Designed as a fast-penetrating stain, this kit provides excellent contrast while preserving nucleic acid integrity and retaining low-abundance mRNAs.

The Arcturus Paradise Plus FFPE LCM Staining Kit (Cat. No. KIT0312J) offers a simple solution to stain formalin-fixed, paraffin-embedded (FFPE) tissues before proceeding with laser capture microdissection (LCM) and downstream molecular analysis. FFPE samples introduce unique challenges for gene expression profiling, including chemical modification and fragmentation of RNA molecules. Archiving of FFPE samples adds to these challenges through increased RNA degradation over time.

The Arcturus HistoGene LCM Immunofluorescence Staining Kit (Cat. No. KIT0420) is designed to retrieve high-quality nucleic acids from immunofluorescently stained, frozen tissue using a quick 15-minute process. The kit provides reagents and slides for convenient and reliable immunofluorescent staining as well as protocols that are streamlined and optimized to maintain nucleic acid quality. The staining kit is used with a biotinylated monoclonal primary antibody against an antigen of choice provided by the user, and Cyanine 3–conjugated streptavidin is included in the kit.

The dual-laser (IR) and UV nature of the ArcturusXT Laser Capture Microdissection System offers ultimate flexibility for tissue preparation. A wide variety of tissues can be mounted on the system and subsequently microdissected off. We offer three types of slides that can be used for microdissection:

• Glass slides—uncharged, uncoated, standard glass slides that are useful for researchers looking to use only the IR laser to isolate their cells of interest. 
• PEN membrane glass slides—enable the combined use of IR LCM and UV Laser Cutting.
• PEN membrane frame slides—provide additional flexibility by allowing the choice of microdissection from non-dehydrated sample preparations, and thick sections.