MitoProbe™ TMRM Assay Kit for Flow Cytometry

Citations & References (4)

Thermo Scientific™

MitoProbe™ TMRM Assay Kit for Flow Cytometry

MitoProbe TMRM Assay Kitには、ミトコンドリア膜電位状態を検出するためのテトラメチルローダミンメチルエステル（TMRM）と、ミトコンドリア膜脱分極を誘導するためのCCCPが含まれています（必要な場合）詳細を見る

製品番号（カタログ番号）	数量
M20036	100 assays

製品番号（カタログ番号） M20036

価格（JPY）

51,400

お問い合わせください ›

100 assays

MitoProbe TMRM Assay Kitには、ミトコンドリア膜電位状態を検出するためのテトラメチルローダミンメチルエステル（TMRM）と、ミトコンドリア膜脱分極を誘導するためのCCCPが含まれています（必要な場合）。

•生細胞中のミトコンドリア膜電位の研究
• 迅速で簡単なプロトコル

ミトコンドリアの健康状態の判断は、ミトコンドリア膜電位の変化を検出することで実現できます。活発なミトコンドリアがある健康な細胞では、TMRMが容易に隔離され、赤オレンジ色の蛍光シグナルが発光されます。アポトーシスが誘発されるか、CCCPなどの治療が適用されると、ミトコンドリア膜が脱分極され、TMRMシグナルが減少します。

検出法蛍光

フォーマットバイアル

数量100 assays

出荷条件湿氷

Emission561

使用対象（アプリケーション）TMRMアッセイ

使用対象 (装置)フローサイトメーター

製品タイプFlow Cytometry Assay Kit

Unit SizeEach

組成および保存条件

1バイアルのTMRM（100試験）
1バイアルの50 mM CCCP
–5℃～–30℃で保存してください。

よくあるご質問（FAQ）

In the MitoProbe TMRM Assay Kit for Flow Cytometry, should CCCP be present when adding the TMRM?

Yes. The CCCP should be first added to the cell sample, incubated for 5 mins, and then the TMRM should be added and incubated for another 30 mins.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

How does the MitoProbe TMRM Assay Kit for Flow Cytometry (Cat. No.M20036) differ from the Image-iT TMRM Reagent (Cat No. I34361)?

The Image-iT TMRM Reagent is provided as a 100 µM solution of TMRM in DMSO (5 vials, 100 µL in each vial). The MitoProbe TMRM Assay Kit for Flow Cytometry provides the TMRM as a 20 µM stock solution in DMSO (1 vial, 100 µL) and a vial of CCCP. Both kits have the stock solutions as a 1000X concentration, but final working concentrations required for imaging applications are 5-fold higher than what is needed for flow cytometry analysis.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

How is TMRM different from the MitoTracker dyes?

TMRM is a dynamic mitochondrial stain. There is no covalent attachment of TMRM to the mitochondria and because of this, it can move in and out of the mitochondria dependent upon changes in the membrane potential. The MitoTracker dyes on the other hand possess a reactive chloromethyl group that allows for their covalent attachment to mitochondria. As a result, once they accumulate within mitochondria that have a normal membrane potential, they are retained.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

引用および参考文献 (4)

引用および参考文献

Abstract

Mitochondrial Delivery of an Anticancer Drug Via Systemic Administration Using a Mitochondrial Delivery System That Inhibits the Growth of Drug-Resistant Cancer Engrafted on Mice.

Authors:Yamada Y, Munechika R, Satrialdi Kubota F, Sato Y, Sakurai Y, Harashima H

Journal:J Pharm Sci

PubMed ID:32376272

'Mitochondrial delivery of an anticancer drug targeting cancer cells would eventually result in cell death. To achieve this, a drug delivery system targeting mitochondria is needed. We recently developed a MITO-Porter, a liposome that delivers its cargo to mitochondria. We reported that such a MITO-Porter could deliver doxorubicin (DOX), an ... More

Zidovudine-Mediated Autophagy Inhibition Enhances Mitochondrial Toxicity in Muscle Cells.

Authors:Lin H, Stankov MV, Hegermann J, Budida R, Panayotova-Dimitrova D, Schmidt RE, Behrens GMN,

Journal:Antimicrob Agents Chemother

PubMed ID:30373793

Nucleoside reverse transcriptase inhibitors (NRTI), such as zidovudine (AZT), are constituents of HIV-1 therapy and are used for the prevention of mother-to-child transmission. Prolonged thymidine analogue exposure has been associated with mitochondrial toxicities to heart, liver, and skeletal muscle. We hypothesized that the thymidine analogue AZT might interfere with autophagy ... More

Lectin-induced oxidative stress in human platelets.

Authors:Signorello MG, Ravera S, Leoncini G

Journal:Redox Biol

PubMed ID:32063518

Previously we have shown that wheat germ agglutinin (WGA) and, with minor potency, Phaseolus vulgaris agglutinin (PHA), but not lens culinarian agglutinin (LCA), induce platelet aggregation, through the PLC?2 activation by the concerted action of src/syk and PI3K/BTK pathways. In this study, we have investigated platelet oxidative stress induced by ... More

Rapid Death of Follicular B Cells and Burkitt Lymphoma Cells Effectuated by Xbp1s.

Authors:Chen YT, Kung JT

Journal:J Immunol

PubMed ID:32376649

BCR-mediated tonic signaling is an indispensable requirement for the survival of follicular B (FOB) cells and Burkitt lymphoma (BL) cells. FOB cells of the I-A