MitoProbe™ Transition Pore Assay Kit, for flow cytometry

Citations & References (16)

Invitrogen™

MitoProbe™ Transition Pore Assay Kit, for flow cytometry

MitoProbe™遷移細孔アッセイキットは、ミトコンドリア透過性遷移細孔開度を測定する実証済みの方法（Petronilli V. et al.1989, 1999）を提供します。このキットには、色素とクエンチャーが含まれており詳細を見る

製品番号（カタログ番号）	数量
M34153	1 kit

製品番号（カタログ番号） M34153

価格（JPY）

141,200

お問い合わせください ›

1 kit

MitoProbe™遷移細孔アッセイキットは、ミトコンドリア透過性遷移細孔開度を測定する実証済みの方法（Petronilli V. et al.1989, 1999）を提供します。このキットには、色素とクエンチャーが含まれており、細胞に簡単にロードできます。健康な細胞では、色素はミトコンドリアに入りますが、クエンチャーは入りません。ミトコンドリアは、ミトコンドリアの細孔の活性化が蛍光の消光を可能にするまで、明るい蛍光が維持されます。

フローサイトメトリー用アポトーシスアッセイの選択ガイドを表示。

研究用にのみ使用できます。診断用には使用いただけません。

励起／発光488

反応数100 Reactions

製品ラインMitoProbe

製品タイプTransition Pore Assay Kit

数量1 kit

出荷条件室温

コンジュゲートCalcein AM

フォーマットチューブ

Unit SizeEach

組成および保存条件

5バイアルのカルセイン、AM（50 μgの粉末／バイアル）、1バイアルのコバルト（II）塩化物六水和物（1.2 mL、生理食塩水80 mM）、1バイアルのイオノマイシン（55 μg）、カルシウム塩、1バイアルのDMSO（1.5 mL）を含みます。

冷凍庫（-5～-30℃）に保存。

よくあるご質問（FAQ）

What are the advantages of flow cytometry?

-Measures data from single cells.
-Data are obtained for a large number of cells, generating a rich statistical analysis of cell populations.
-Because single cells are measured, it will reveal heterogeneity within a population.
-With the ability to multiplex, small sub-populations can be identified.
-Thousands of cells can be analyzed rapidly.
-It is ideally suited for blood samples and other cells in suspension.
-Data can be re-analyzed multiple times after acquisition.
-Flow cytometry files (FCS) can be archived.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

What kinds of applications can I run on a flow cytometer?

There are several applications, some of which include immunophenotyping, cell cycle analysis, apoptosis assays such as annexin V staining, CellEvent Caspase-3/7 assay, and TUNEL assay, cell viability, proliferation assays such as CellTrace assay and Click-iT EdU assay, measurements of mitochondrial potential with MitoProbe assays, and cell counting using counting beads.

Find additional tips, troubleshooting help, and resources within our Flow Cytometry Support Center.

引用および参考文献 (16)

引用および参考文献

Abstract

Cysteine 203 of cyclophilin D is critical for cyclophilin D activation of the mitochondrial permeability transition pore.

Authors:Nguyen TT, Stevens MV, Kohr M, Steenbergen C, Sack MN, Murphy E,

Journal:J Biol Chem

PubMed ID:21930693

The mitochondrial permeability transition pore (mPTP) opening plays a critical role in mediating cell death during ischemia/reperfusion (I/R) injury. Our previous studies have shown that cysteine 203 of cyclophilin D (CypD), a critical mPTP mediator, undergoes protein S-nitrosylation (SNO). To investigate the role of cysteine 203 in mPTP activation, we ... More

Transient and long-lasting openings of the mitochondrial permeability transition pore can be monitored directly in intact cells by changes in mitochondrial calcein fluorescence.

Authors:Petronilli V, Miotto G, Canton M, Brini M, Colonna R, Bernardi P, Di Lisa F

Journal:Biophys J

PubMed ID:9929477

'The occurrence and the mode of opening of the mitochondrial permeability transition pore (MTP) were investigated directly in intact cells by monitoring the fluorescence of mitochondrial entrapped calcein. When MH1C1 cells and hepatocytes were loaded with calcein AM, calcein was also present within mitochondria, because (i) its mitochondrial signal was ... More

Calpain and caspase orchestrated death signal to accomplish apoptosis induced by resveratrol and its novel analog hydroxystilbene-1 [correction of hydroxstilbene-1] in cancer cells.

Authors:Guha P, Dey A, Dhyani MV, Sen R, Chatterjee M, Chattopadhyay S, Bandyopadhyay SK,

Journal:J Pharmacol Exp Ther

PubMed ID:20484155

'Stomach ulceration is a major side effect of most chemopreventive drugs. We have established that although resveratrol is a promising chemopreventive compound, it delays the ulcer healing process. However, its analog hydroxystilbene-1 (HST-1) was devoid of such an ulcerogenic side effect. Consequently, here we tried to explore the chemopreventive efficacy ... More

Secondary coenzyme Q10 deficiency triggers mitochondria degradation by mitophagy in MELAS fibroblasts.

Authors:Cotán D, Cordero MD, Garrido-Maraver J, Oropesa-Ávila M, Rodríguez-Hernández A, Gómez Izquierdo L, De la Mata M, De Miguel M, Lorite JB, Infante ER, Jackson S, Navas P, Sánchez-Alcázar JA,

Journal:FASEB J

PubMed ID:21551238

Mitochondrial encephalomyopathy, lactic acidosis, and stroke-like episodes (MELAS) is a mitochondrial disease most usually caused by point mutations in tRNA genes encoded by mtDNA. Here, we report on how this mutation affects mitochondrial function in primary fibroblast cultures established from 2 patients with MELAS who harbored the A3243G mutation. Both ... More

Imaging the mitochondrial permeability transition pore in intact cells.

Authors:Petronilli V, Miotto G, Canton M, Colonna R, Bernardi P, Di Lisa F

Journal:Biofactors

PubMed ID:9914828

The involvement of mitochondrial permeability transition pore (MTP) in cellular processes is generally investigated by indirect means, such as changes in mitochondrial membrane potential or pharmacological inhibition. However, such effects could not be related univocally to MTP. In addition, source of errors could be represented by the increased retention of ... More

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