Neon™ NxT Electroporation System 10-μL Kit with 1-Channel Tubes
Neon™ NxT Electroporation System 10-μL Kit with 1-Channel Tubes
Neon™ NxT Electroporation System 10-μL Kit with 1-Channel Tubes
Neon™ NxT Electroporation System 10-μL Kit with 1-Channel Tubes
Neon™ NxT Electroporation System 10-μL Kit with 1-Channel Tubes
Neon™ NxT Electroporation System 10-μL Kit with 1-Channel Tubes
Neon™ NxT Electroporation System 10-μL Kit with 1-Channel Tubes
Invitrogen™

Neon™ NxT Electroporation System 10-μL Kit with 1-Channel Tubes

The Neon NxT Electroporation System 10-μL Kit is designed specifically for use with the Neon NxT Electroporation System.
製品番号(カタログ番号)反応数
N109696 x 2 Reactions
N102525 x 2 Reactions
製品番号(カタログ番号) N1096
価格(JPY)
246,000
Each
お問い合わせください ›
反応数:
96 x 2 Reactions

The Neon NxT Electroporation System 10-μL Kit with 1-Channel Tubes is designed specifically for use with the Neon NxT Electroporation System (Cat. Nos. NEON1S, NEON1SK, NEON18S, NEON18SK). Use this kit for transfection volumes of 10 μL, containing 5 x 104–2 x 105 adherent cells or 1 x 105–5 x 105 suspension cells. Cells that have been transfected using the included 10-μL Neon NxT tips are ready to be washed and plated into a multi-well culture dish.

研究用途にのみご使用ください。診断目的には使用できません。
仕様
使用対象(アプリケーション)Transfection
使用対象 (装置)Neon™ NxT Electroporation System
反応数96 x 2 Reactions
製品タイプPipette Tip
数量96 x 2 reactions
容量(メートル法)サンプル10 μL
細胞タイプStem Cells, Primary Cells, Hard-to-Transfect Cells
フォーマットElectroporation Pipette Tip
Transfection Techniqueエレクトロポレーション
Unit SizeEach
組成および保存条件
• 4 x 1 mL Resuspension R Buffer; store at 4°C
• 4 x 1 mL Resuspension T Buffer; store at 4°C
• 4 x 1 mL Resuspension Genome Editing Buffer; store at 4°C
• 2 x 100 mL Electrolytic E10 Buffer; store at 4°C
• 96 reaction tips
• 32 electroporation 1-channel tubes

よくあるご質問(FAQ)

Should I warm Neon NxT buffers before use?

We recommend warming the buffers to room temperature before use.

Find additional tips, troubleshooting help, and resources within our Transfection Support Center.

Can I aliquot and store Neon NxT buffers?

We recommend storing Neon NxT buffers in the original containers at 4 degrees C.

Find additional tips, troubleshooting help, and resources within our Transfection Support Center.

What temperature should Neon NxT buffers be stored at?

Neon NxT buffers should be stored at 4 degrees C. Temperature fluctuations and storage at room temperature have been shown to increase the chances for precipitate formation. Therefore, the desired amount of buffer for an experiment should be removed from the buffer bottle and the bottle should be immediately returned to 4 degrees C.

Find additional tips, troubleshooting help, and resources within our Transfection Support Center.

What state should the cells be in for best results with the Neon Nxt Electoporation System?

For best results with the Neon Nxt Electoporation System, the cells should be healthy (not stressed) and actively dividing so that the nuclear membrane integrity is already weakened for payload needing to enter the nucleus. Consider “starving” the cells of serum or other essential nutrients.

Find additional tips, troubleshooting help, and resources within our Transfection Support Center.

I'm experiencing arcing issues with the Neon Nxt Electoporation System. What could be causing this?

Arcing could be caused by high voltage or pulse length settings, high salt or contaminants in the DNA sample, incorrect cell density, and air bubbles formed during mixing of cell samples. Mix samples gently to avoid air bubble formation and pipette samples in a slow, smooth motion to avoid air uptake.

Find additional tips, troubleshooting help, and resources within our Transfection Support Center.