NanoOrange™ Protein Quantitation Kit, 200-2,000 assays

Citations & References (29)

Invitrogen™

NanoOrange™ Protein Quantitation Kit, 200-2,000 assays

NanoOrangeタンパク質定量キットには、タンパク質定量化用の非常に高感度で簡単なアッセイが含まれており、溶液内のわずか10 ng/mLのタンパク質を検出できます。蛍光色素は、分光光度計やマイクロプレートリーダーでの使用に適しています。複雑な脂質環境でリポタンパク質またはタンパク質の検出には、当社のCBQCAタンパク質定量キット（C-6667）をご確認ください詳細を見る

製品番号（カタログ番号）	数量
N6666	1 Kit

製品番号（カタログ番号） N6666

価格（JPY）

101,400

お問い合わせください ›

1 Kit

一括またはカスタム形式をリクエストする

NanoOrangeタンパク質定量キットには、タンパク質定量化用の非常に高感度で簡単なアッセイが含まれており、溶液内のわずか10 ng/mLのタンパク質を検出できます。蛍光色素は、分光光度計やマイクロプレートリーダーでの使用に適しています。複雑な脂質環境でリポタンパク質またはタンパク質の検出には、当社のCBQCAタンパク質定量キット（C-6667）をご確認ください。

研究用にのみ使用できます。診断用には使用いただけません。

アッセイタンパク質定量アッセイ

使用対象（アプリケーション）タンパク質定量

使用対象 (装置)マイクロプレートリーダー

製品ラインNanoOrange

製品タイプProtein Quantitation Assay

数量1 Kit

対応可能対象200から2000のアッセイ

検出法蛍光

Unit SizeEach

よくあるご質問（FAQ）

When preparing the serial dilutions for the standard curve for the NanoOrange Protein Quantitation Kit (Cat. No. N6666), can they first be diluted in buffer (e.g., Tris) or do they have to be diluted in 1X NanoOrange working solution?

All samples and standards need to be diluted in 1X NanoOrange working solution, not in any other buffer.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

My buffer or components of my buffer are not listed in the compatibility table for my protein assay. What should I do?

You can test the tolerance of the assay for your specific buffer formulation. For in-house generated compatibility information, substances were considered compatible at the indicated concentration in the Standard Test Tube Protocol (found in the manual for each protein assay) if the error in protein concentration estimation caused by the presence of the substance was less than or equal to 10%. The substances were tested using WR prepared immediately before each experiment. Blank-corrected 562nm absorbance measurements (for a 1000µg/mL BSA standard + substance) were compared to the net 562nm measurements of the same standard prepared in 0.9% saline.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

All the components of my sample buffer are at or below the indicated compatible concentration for my protein assay, but I am still seeing too much/too little color development. What could be the problem?

It is possible to have a substance additive affect such that even though a single component is present at a concentration below its listed compatibility, a sample buffer containing a combination of substances could interfere with the assay. You should take steps to eliminate or minimize the effects of the interfering substance(s) by diluting or removing the substance.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

My protein assay is not developing color or is developing too much color. What can I do?

Refer to the information in the product-specific instruction booklet or our Tech Tip: Protein Quantitation Assay Compatibility Table (https://assets.thermofisher.com/TFS-Assets/LSG/Application-Notes/TR0068-Protein-assay-compatibility.pdf).

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

My spectrophotometer doesn’t have a filter set for the absorbance maximum. Can I use an alternate wavelength to read the protein assay?

Often, an alternative wavelength can be used, although the slope of the standard curve and the overall assay sensitivity will most likely be reduced. Our Tech Tip (https://tools.thermofisher.com/content/sfs/brochures/TR0025-Protein-assay-spectra.pdf) offers additional information on determining acceptable wavelengths for measuring protein assays.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

View all NanoOrange™ Protein Quantitation Kit, 200-2,000 assays FAQs

引用および参考文献 (29)

引用および参考文献

Abstract

Authors:

Journal:

PubMed ID:10652312

Identification of cyclic AMP-regulated genes in Mycobacterium tuberculosis complex bacteria under low-oxygen conditions.

Authors:Gazdik MA, McDonough KA

Journal:J Bacteriol

PubMed ID:15805514

'Mycobacterium tuberculosis is the etiological agent of tuberculosis (TB), which kills approximately 2 million people a year despite current treatment options. A greater understanding of the biology of this bacterium is needed to better combat TB disease. The M. tuberculosis genome encodes as many as 15 adenylate cyclases, suggesting that ... More

Regulation of collagen deposition and lysyl oxidase by tumor necrosis factor-alpha in osteoblasts.

Authors:Pischon N, Darbois LM, Palamakumbura AH, Kessler E, Trackman PC

Journal:J Biol Chem

PubMed ID:15138266

'Tumor necrosis factor-alpha (TNF-alpha) inhibits osteoblast function in vitro by inhibiting collagen deposition. Studies generally support that TNF-alpha does not inhibit collagen biosynthesis by osteoblasts but that collagen deposition is in some way diminished. The study investigated TNF-alpha regulation of biosynthetic enzymes and proteins crucial for posttranslational extracellular collagen maturation ... More

Control of neuronal size homeostasis by trophic factor-mediated coupling of protein degradation to protein synthesis.

Authors:Franklin JL, Johnson EM

Journal:J Cell Biol

PubMed ID:9732291

'We demonstrate that NGF couples the rate of degradation of long-lived proteins in sympathetic neurons to the rate of protein synthesis. Inhibiting protein synthesis rate by a specific percentage caused an almost equivalent percentage reduction in the degradation rate of long-lived proteins, indicating nearly 1:1 coupling between the two processes. ... More

Key role of conserved histidines in recombinant mouse beta-carotene 15,15'-monooxygenase-1 activity.

Authors:Poliakov E, Gentleman S, Cunningham FX, Miller-Ihli NJ, Redmond TM

Journal:J Biol Chem

PubMed ID:15951442

'Alignment of sequences of vertebrate beta-carotene 15,15''-monooxygenase-1 (BCMO1) and related oxygenases revealed four perfectly conserved histidines and five acidic residues (His172, His237, His308, His514, Asp52, Glu140, Glu314, Glu405, and Glu457 in mouse BCMO1). Because BCMO1 activity is iron-dependent, we propose that these residues participate in iron coordination and therefore are ... More

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