Oligo(dT)16プライマーは、濃度が50 μMで、pH 7.0、10 mM TEの100 μLで供給されます。−5~−30℃で保管してください。繰り返しの凍結や解凍を避けるためにアリコートを使用しています。
よくあるご質問(FAQ)
Can Oligo d(T)16 RT primers be used with the TaqMan Human Endogenous Control Plate (P/N 4309199)?
No. The reverse transcription of total RNA to cDNA must be done with random hexamers or random primers (or a mixture of random and oligo dT primers) because the 18S rRNA assay cannot evaluate poly A+ purified RNA samples as most of the ribosomal RNA has been removed. The plate has been designed to evaluate only total RNA.