DryEase™ Mini-Gel Drying System
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Invitrogen™

DryEase™ Mini-Gel Drying System

DryEase™ ミニゲル乾燥システムは、シンプルで使いやすいキットで、受動的蒸発を利用してゲルを均一に乾燥させます。このキットを使用すると、2 つのオープンフレームの間のベース上にゲルをアセンブルできるため、ゲルの外側の両方の表面が空気にさらされます。その結果、乾燥が均一になり詳細を見る
製品番号(カタログ番号)数量
NI23871 system
製品番号(カタログ番号) NI2387
価格(JPY)
43,100
Each
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数量:
1 system
DryEase™ ミニゲル乾燥システムは、シンプルで使いやすいキットで、受動的蒸発を利用してゲルを均一に乾燥させます。このキットを使用すると、2 つのオープンフレームの間のベース上にゲルをアセンブルできるため、ゲルの外側の両方の表面が空気にさらされます。その結果、乾燥が均一になり、亀裂が最小限に抑えられます。この特許取得済みのGel Dry™乾燥溶液は、水の放出速度を調節することで亀裂のないゲルを実現します。DryEase™ システムを使用すると、ゲルのすべてのポイントで均一なテンションで一晩中乾燥し、ひび割れを防ぎます。システムのすべてのコンポーネントは別売りです。

アプリケーション:DryEase™ システムで乾燥したゲルは、蛍光、デンシトメトリー、オートラジオグラフィー、または恒久的なゲル保存に適しています。
研究用途にのみご使用ください。診断目的には使用できません。
仕様
製品ラインDryEase, Novex
製品タイプミニゲル乾燥システム
数量1 system
品質保持期間1年間
使用対象 (装置)DryEase Mini-Gel Drying System
Unit SizeEach
組成および保存条件
DryEase™ ミニゲル乾燥キットには、8個のGel-Dry™クランプ付きDryEase™ ミニゲル乾燥フレーム2個、DryEase™ ミニゲル乾燥ベース、DryEase™ ミニセロファン(200枚のプレカットシート)、Gel-Dry™ 乾燥液(500 ml)が含まれています。溶液は室温で保管してください。適切な保存条件であれば、1年間安定していることが保証されています。

よくあるご質問(FAQ)

I dried my gel using your DryEase Mini Gel Drying system and the gel cracked during drying. Can you please help?

Please see below for possible causes and solutions:

- Air trapped between cellophane layers. Apply adequate Gel-Drying solution to prevent bubbles between the gel and cellophane assembly.
- Tension on cellophane too strong. Take care not to stretch the cellophane during dryer assembly.
- Rough edges or small cracks on gel edges. Trim rough edges from the gel to eliminate starting points for cracks.
- Cellophane slips during drying. Make sure clamps hold all four edges of the dryer assembly firmly.

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

I am using your DryEase Mini Gel Drying system and would like to peel off the cellophane after drying the gel but it seems really difficult. Can you please offer some tips?

Usually it is not necessary to peel off the cellophane. If your signal is very faint and you are concerned about getting an exposure, you can rub the top surface of your gel with a damp towel and then peel off the cellophane. Then air dry the gel for a few minutes and perform autoradiography.

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

I dried my gel using Gel-Drying Solution and it dried milky white. Can the gel be recovered?

Gels turn white when they are dried too rapidly, usually in an environment that is too ventilated, drafty, or warm. You can remedy this problem by dabbing the gel with deionized water on a Kimwipe tissue to rehydrate the white areas. Re-dry the gel in a dark, dry, non-drafty place, such as a drawer or cabinet.

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

What is the pore size of the DryEase cellophane?

The pore size of DryEase cellophane is 40 microns.

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

Do you offer a system for drying of protein gels?

We offer the following systems for drying of protein gels:

DryEase Mini Gel Drying System (Cat. No. NI2387): Simple-to-use kit for even, crack-free drying of mini gels that dries gels evenly, using passive evaporation. Gels dried with this system are suitable for fluorography, densitometry, autoradiography, or permanent gel storage. Each DryEase Mini-Gel Drying System contains the following components:

- 2-DryEase Mini-Gel Drying Frames (includes 2 frames + 8 clamps) (Cat. No. NI2380)
- 1-DryEase Mini-Gel Drying Base (Cat. No. NI2300)
- 200-DryEase Mini Cellophane precut sheets (Cat. No. NC2380)
- 500 mL Gel-Dry Drying Solution (Cat. No. NI2100)
Large Gel Drying Kit (Cat. No. NI2207): Provides the same even, crack-free gel drying seen with the DryEase Mini-Gel Drying System but is designed to accommodate larger gels or 4 mini-gels. A base for the system is not required.

Each Large Gel Drying Kit contains the following components:

- 1-DryEase Large Gel Drying Frame (includes 8 clamps) (Cat. No. NI2200)
- 100-DryEase Large Cellophane precut sheets (Cat. No. NC2200)
- 500 mL Gel-Dry Drying Solution (Cat. No. LC4025)

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

引用および参考文献 (1)

引用および参考文献
Abstract
In organello formaldehyde crosslinking of proteins to mtDNA: identification of bifunctional proteins.
Authors:Kaufman BA, Newman SM, Hallberg RL, Slaughter CA, Perlman PS, Butow RA
Journal:Proc Natl Acad Sci U S A
PubMed ID:10869431
The segregating unit of mtDNA is a protein-DNA complex called the nucleoid. In an effort to understand how nucleoid proteins contribute to mtDNA organization and inheritance, we have developed an in organello formaldehyde crosslinking procedure to identify proteins associated with mtDNA. Using highly purified mitochondria, we observed a time-dependent crosslinking ... More