NuPAGE™ MES SDS Buffer Kit (for Bis-Tris Gels)
NuPAGE™ MES SDS Buffer Kit (for Bis-Tris Gels)
Citations & References (1)
Invitrogen™

NuPAGE™ MES SDS Buffer Kit (for Bis-Tris Gels)

NuPAGE MES SDSバッファーキットは、NuPAGE Bis-Trisゲルでの小∼中サイズのタンパク質の分離用に設計されており、以下のバッファーを含んでいます。• NuPAGE MES SDSランニングバッファー詳細を見る
テクニカルサポートオーダーサポート
製品番号（カタログ番号）数量
NP00601キット
製品番号（カタログ番号） NP0060
価格（JPY）
22,600
Each
お問い合わせください ›
数量:
1キット
NuPAGE MES SDSバッファーキットは、NuPAGE Bis-Trisゲルでの小∼中サイズのタンパク質の分離用に設計されており、以下のバッファーを含んでいます。
• NuPAGE MES SDSランニングバッファー（20X、500 mL、カタログ番号NP0002）
• NuPAGEサンプル還元剤（10X、250 µL、カタログ番号NP0004）
• NuPAGE抗酸化剤（カタログ番号NP0005）
• NuPAGE LDSサンプルバッファー（4X、10 mL、カタログ番号NP0007）

NuPAGE Bis-TrisゲルでMESおよびMOPを使用してタンパク質の移動パターンを比較

SDS-PAGEに利用可能なすべてのバッファーと試薬を確認

研究用途にのみご使用ください。診断目的には使用できません。
仕様
ゲルタイプSDS PAGEゲル
数量1キット
Gel CompatibilityNovex™ Tricine Gel
製品ラインNuPAGE
製品タイプBuffer Kit
Unit SizeEach
組成および保存条件
付属品
• NuPAGE™ MES SDSランニングバッファー(20X、500 ml）
• NuPAGE™サンプル還元剤(10X、250 µl、500mMジチオトリトールを含む）
• NuPAGE™抗酸化剤(15mL)
• NuPAGE™ LDSサンプルバッファー（10 ml）
それぞれの保管温度は4℃です。NuPAGE™ LDSサンプルバッファーを使用する前に25℃に温めてください。

よくあるご質問（FAQ）

Can I prepare my protein sample with the reducing agent and store it for future use?

DTT is not stable, so it must be added and the reduction performed just prior to loading your samples.

Find additional tips, troubleshooting help, and resources within our Protein Gel 1D Electrophoresis Support Center.

My LDS or SDS sample buffer precipitates when stored at 4 degrees C. Can I warm it up? Can I store it at room temperature?

Precipitation of the LDS or SDS at 4 degrees C is normal. Bring the buffer to room temperature and mix until the LDS/SDS goes into solution. If you do not want to wait for it to dissolve, you can store the sample buffer at room temperature.

Find additional tips, troubleshooting help, and resources within our Protein Gel 1D Electrophoresis Support Center.

How are Bolt gels different than NuPAGE gels?

While they are both Bis-Tris based gels, the chemistries are very different since Bolt gels are optimized for western blotting. Another key difference is the wedge well design of the Bolt gels, which allows larger sample volumes to be loaded.

Find additional tips, troubleshooting help, and resources within our Protein Gel 1D Electrophoresis Support Center.

What is the advantage of NuPAGE Gels over regular Tris-Glycine gels?

The neutral operating pH of the NuPAGE Gels and buffers provides following advantages over the Laemmli system:
-Longer shelf life of 8-12 months due to improved gel stability
-Improved protein stability during electrophoresis at neutral pH resulting in sharper band resolution and accurate results (Moos et al, 1998)
-Complete reduction of disulfides under mild heating conditions (70 degrees C for 10 min) and absence of cleavage of asp-pro bonds using the NuPAGE LDS Sample buffer (pH > 7.0 at 70 degrees C)
-Reduced state of the proteins maintained during electrophoresis and blotting of the proteins by the NuPAGE Antioxidant
Please refer to the following paper: Moos M Jr, Nguyen NY, Liu TY (1988) Reproducible High Yield Sequencing of Proteins Electrophoretically Separated and Transferred to an Inert Support. J Biol Chem 263:6005-6008.

Find additional tips, troubleshooting help, and resources within our Protein Gel 1D Electrophoresis Support Center.

Can beta-mercaptoethanol (BME) be used rather than DTT as the reducing agent in the NuPAGE LDS Sample Buffer?

Either BME or DTT can be used in the NuPAGE LDS Sample Buffer.

Make sure that a fresh solution of BME is used. FINAL concentration:

DTT 50-100 mM

BME 2-5%

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

View all NuPAGE™ MES SDS Buffer Kit (for Bis-Tris Gels) FAQs

引用および参考文献 (1)

引用および参考文献
Abstract
Spermidine but not spermine is essential for hypusine biosynthesis and growth in Saccharomyces cerevisiae: Spermine is converted to spermidine in vivo by the FMS1-amine oxidase.
Authors:Chattopadhyay MK, Tabor CW, Tabor H,
Journal:Proc Natl Acad Sci U S A
PubMed ID:14617780
In our earlier work we showed that either spermidine or spermine could support the growth of spe2Delta or spe3Delta polyamine-requiring mutants, but it was unclear whether the cells had a specific requirement for either of these amines. In the current work, we demonstrate that spermidine is specifically required for the ... More