NuPAGE™ Bis-Tris Mini Protein Gels, 4–12%, 1.0–1.5 mm

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NuPAGE™ Bis-Tris Mini Protein Gels, 4–12%, 1.0–1.5 mm

Citations & References (15)

Invitrogen™

NuPAGE™ Bis-Tris Mini Protein Gels, 4–12%, 1.0–1.5 mm

Name: NuPAGE™ Bis-Tris Mini Protein Gels, 4–12%, 1.0–1.5 mm
SKU: NP0321BOX

Invitrogen NuPAGE ビス－トリスタンパク質ゲルはプレキャストポリアクリルアミドゲルであり、高分解能とサンプルの完全性で、分子量の広いタンパク質分離を実現します。

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表示を変更

製品番号（カタログ番号）	ウェル	Gel Thickness	数量
NP0321BOX	10ウェル	1.0 mm	10ゲル／箱
NP0321PK2	10ウェル	1.0 mm	2ゲル／箱
NP0322BOX	12ウェル	1.0 mm	10ゲル／箱
NP0322PK2	12ウェル	1.0 mm	2ゲル／箱
NP0323BOX	15ウェル	1.0 mm	10ゲル／箱
NP0323PK2	15ウェル	1.0 mm	2ゲル／箱
NP0324BOX	1ウェル	1.0 mm	10ゲル／箱
NP0326BOX	2Dウェル	1.0 mm	10ゲル／箱
NP0327BOX	9ウェル	1.0 mm	10ゲル／箱
NP0329BOX	17ウェル	1.0 mm	10ゲル／箱
NP0329PK2	17ウェル	1.0 mm	2ゲル／箱
NP0330BOX	IPGウェル	1.0 mm	10ゲル／箱
NP0335BOX	10ウェル	1.5 mm	10ゲル／箱
NP0335PK2	10ウェル	1.5 mm	2ゲル／箱
NP0336BOX	15ウェル	1.5 mm	10ゲル／箱
NP0336PK2	15ウェル	1.5 mm	2ゲル／箱

16 オプション

製品番号（カタログ番号） NP0321BOX

価格（JPY）

27,500

Each

お問い合わせください ›

ウェル:

10ウェル

Gel Thickness:

1.0 mm

数量:

10ゲル／箱

Invitrogen NuPAGE ビス－トリスタンパク質ゲルはプレキャストポリアクリルアミドゲルであり、高分解能とサンプルの完全性で、分子量の広いタンパク質分離を実現します。これらのプレキャストゲルは、タンパク質の完全性が重要なアプリケーションに最適です。従来のトリス-グリシンゲルとは異なり、NuPAGEビス-トリスゲルのpH環境は中性であるため、タンパク質修飾を最小限に抑えます。NuPAGE ビス－トリスゲルは、シーケンシング、質量分析、およびタンパク質の完全性が重要なその他の技術用にタンパク質を調製する場合に使用します。

NuPAGE Bis-Trisゲルの特長：
•より優れたタンパク質の統合性—最適化されたサンプル調製プロセスタンパク質により、タンパク質が保護されます
• 分子量の幅広い分離—適切なゲルと泳動バッファーを選択して、タンパク質を最適に分離します。
• 迅速な泳動—タンパク質の分離をわずか 35分で行えます。
• 長い製品寿命—NuPAGE ビス－トリスゲルは室温で 12ヵ月以上保存できます。

タンパク質分離に適した NuPAGE ビス－トリスゲルを選択してください。
ゲルと泳動バッファーの適切な組み合わせを選択することで、タンパク質の最適な分離が得られます。NuPAGEビス-トリスタンパク質ゲルには、4種類のポリアクリルアミド濃度があります：8%、10%、12%、および 4–12% のグラジエント。ゲルのサイズは、ミニ（8 cm×8 cm）またはミディ（8.7 cm×13.3 cm）の2種類があり、厚さは1.0 mm（ミニゲルおよびミディゲル）または1.5 mm（ミニゲルフォーマットのみ）です。また、NuPAGE Bis-Trisゲルには、複数のウェルフォーマットがあります。

NuPAGE Bis-Trisゲルは、変性ゲル電気泳動アプリケーション用に調製されています。最適なサンプル調製には、NuPAGE LDSサンプルバッファー（NP0007）およびNuPAGEサンプル還元剤（NP0004）をご使用ください。泳動バッファーにNuPAGE抗酸化剤（NP0005）を使用すると、泳動中のタンパク質の還元状態を維持し、バンドのシャープさを最大限に高めます。このゲルは、NuPAGE MES SDS Running Buffer（NP0002）を使用して泳動することで、少量のタンパク質の分離を促します。また、NuPAGE MOPS SDS Running Buffer（NP000102）を使用して中～大きいサイズのタンパク質を分離することができます。

タンパク質を膜に転写する場合は、Mini Blot Module（B1000）またはXCell II Blot Module（EI9051）を用いて、従来のウェットトランスファーにNuPAGE Transfer Buffer（NP00061）を使用することを推奨します。また、高速セミドライ転写にはInvitrogen Power Blotter、または高速ドライ転写にはiBlot 2 Gel転写装置（IB21001）を使用することも可能です。

For Research Use Only. Not for use in diagnostic procedures.

仕様

Gel Thickness1.0 mm

長さ（メートル法）8 cm

分離モード分子量

製品ラインNuPAGE

数量10ゲル／箱

推奨アプリケーション変性

サンプル充填量最大25 µL

品質保持期間12カ月

出荷条件室温

保存要件4–25 ℃ にて保管してください。冷凍不可。

幅（メートル法）8 cm

使用対象 (装置)Mini Gel Tank, XCell SureLock Mini-Cell

ゲル濃度4 ～ 12%

ゲルサイズミニ

ゲルタイプBis-Tris

分離範囲3.5～260 kDa

分離タイプ変性

ウェル10ウェル

Unit SizeEach

よくあるご質問（FAQ）

Can I prepare my protein sample with the reducing agent and store it for future use?

DTT is not stable, so it must be added and the reduction performed just prior to loading your samples.

Find additional tips, troubleshooting help, and resources within our Protein Gel 1D Electrophoresis Support Center.

My LDS or SDS sample buffer precipitates when stored at 4 degrees C. Can I warm it up? Can I store it at room temperature?

Precipitation of the LDS or SDS at 4 degrees C is normal. Bring the buffer to room temperature and mix until the LDS/SDS goes into solution. If you do not want to wait for it to dissolve, you can store the sample buffer at room temperature.

Find additional tips, troubleshooting help, and resources within our Protein Gel 1D Electrophoresis Support Center.

How are Bolt gels different than NuPAGE gels?

While they are both Bis-Tris based gels, the chemistries are very different since Bolt gels are optimized for western blotting. Another key difference is the wedge well design of the Bolt gels, which allows larger sample volumes to be loaded.

Find additional tips, troubleshooting help, and resources within our Protein Gel 1D Electrophoresis Support Center.

What is the advantage of NuPAGE Gels over regular Tris-Glycine gels?

The neutral operating pH of the NuPAGE Gels and buffers provides following advantages over the Laemmli system:
-Longer shelf life of 8-12 months due to improved gel stability
-Improved protein stability during electrophoresis at neutral pH resulting in sharper band resolution and accurate results (Moos et al, 1998)
-Complete reduction of disulfides under mild heating conditions (70 degrees C for 10 min) and absence of cleavage of asp-pro bonds using the NuPAGE LDS Sample buffer (pH > 7.0 at 70 degrees C)
-Reduced state of the proteins maintained during electrophoresis and blotting of the proteins by the NuPAGE Antioxidant
Please refer to the following paper: Moos M Jr, Nguyen NY, Liu TY (1988) Reproducible High Yield Sequencing of Proteins Electrophoretically Separated and Transferred to an Inert Support. J Biol Chem 263:6005-6008.

Find additional tips, troubleshooting help, and resources within our Protein Gel 1D Electrophoresis Support Center.

What is the maximum sample volume and concentration that may be loaded into the Invitrogen precast gel wells?

1-well, 1 mm: 700 µL (no more than 12 µg protein or 2.4 µg DNA per band)

2D-well, 1 mm: 400 µL or 1.5 mm: 600 µµL (no more than 12 µg protein or 2.0 µg DNA per band)

5-well, 1 mm: 60 µL (no more than 2 µg protein or 400 ng µg DNA per band)

9-well, 1 mm: 28 µL (no more than 0.5 µg protein or 100 ng DNA per band)

10-well, 1 mm: 25 µL or 1.5 mm: 37 µL (no more than 0.5 µg protein or 100 ng DNA per band)

12-well, 1 mm: 20 µL (no more than 0.5 µg protein or 100 ng DNA per band)

15-well, 1 mm: 15 µL or 1.5 mm: 25 µL (no more than 0.5 µg protein or 100 ng DNA per band)

17-well 1 mm: 15 µL (no more than 0.5 µg protein per band)

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

View all NuPAGE™ Bis-Tris Mini Protein Gels, 4–12%, 1.0–1.5 mm FAQs

引用および参考文献 (15)

引用および参考文献

Abstract

The mRNA export machinery requires the novel Sac3p-Thp1p complex to dock at the nucleoplasmic entrance of the nuclear pores.

Authors:Fischer T, StrÃ¤sser K, RÃ¡cz A, Rodriguez-Navarro S, Oppizzi M, Ihrig P, Lechner J, Hurt E,

Journal:EMBO J

PubMed ID:12411502

'Yra1p and Sub2p are components of the TREX complex, which couples transcription elongation with nuclear export of mRNAs. Here, we report a genetic interaction between Yra1p and a conserved protein Sac3p, which previously was found to interact with Sub2p. In vivo, Sac3p forms a stable complex with Thp1p, which was ... More

Rapid purification and mass spectrometric characterization of mitochondrial NADH dehydrogenase (Complex I) from rodent brain and a dopaminergic neuronal cell line.

Authors:Schilling B, Bharath M M S, Row RH, Murray J, Cusack MP, Capaldi RA, Freed CR, Prasad KN, Andersen JK, Gibson BW,

Journal:Mol Cell Proteomics

PubMed ID:15591592

'Oxidative stress and mitochondrial dysfunction signify important biochemical events associated with the loss of dopaminergic neurons in Parkinson''s disease (PD). Studies using in vitro and in vivo PD models or tissues from diseased patients have demonstrated a selective inhibition of mitochondrial NADH dehydrogenase (Complex I of the OXPHOS electron transport ... More

Intestinal trefoil factor confers colonic epithelial resistance to apoptosis

Authors:Taupin DR, Kinoshita K, Podolsky DK

Journal:Proc Natl Acad Sci U S A

PubMed ID:10639160

'Intestinal trefoil factor (ITF) is an essential regulator of colonic epithelial restitution, the rapid migration of colonocytes over mucosal wounds. High levels of ITF are frequently present in colorectal cancers and derived cell lines. Mucosal restitution requires the detachment of epithelium from substrate, which would be expected to induce apoptosis. ... More

2F3 monoclonal antibody recognizes the O26 O-antigen moiety of the lipopolysaccharide of enterohemorrhagic Escherichia coli strain 4276.

Authors:Szalo IM, Taminiau B, Goffaux F, Pirson V, McCappin J, Ball HJ, Mainil JG,

Journal:Clin Diagn Lab Immunol

PubMed ID:15138178

'Enterohemorrhagic Escherichia coli (EHEC) and enteropathogenic E. coli (EPEC) organisms are groups of pathogenic strains whose infections are characterized by a typical lesion of enterocyte attachment and effacement. They are involved in enteric diseases both in humans and in animals, and EHEC strains can be responsible for hemolytic uremic syndrome ... More

Human parainfluenza virus type 3 (PIV3) expressing the hemagglutinin protein of measles virus provides a potential method for immunization against measles virus and PIV3 in early infancy.

Authors:Durbin AP, Skiadopoulos MH, McAuliffe JM, Riggs JM, Surman SR, Collins PL, Murphy BR

Journal:J Virol

PubMed ID:10888621

'Recombinant human parainfluenza virus type 3 (PIV3) was used as a vector to express the major protective antigen of measles virus, the hemagglutinin (HA) glycoprotein, in order to create a bivalent PIV3-measles virus that can be administered intranasally. The measles virus HA open reading frame (ORF) was inserted as an ... More

View all NuPAGE™ Bis-Tris Mini Protein Gels, 4–12%, 1.0–1.5 mm citations