Can I prepare my protein sample with the reducing agent and store it for future use?
DTT is not stable, so it must be added and the reduction performed just prior to loading your samples.
Find additional tips, troubleshooting help, and resources within our Protein Gel 1D Electrophoresis Support Center.
My LDS or SDS sample buffer precipitates when stored at 4 degrees C. Can I warm it up? Can I store it at room temperature?
Precipitation of the LDS or SDS at 4 degrees C is normal. Bring the buffer to room temperature and mix until the LDS/SDS goes into solution. If you do not want to wait for it to dissolve, you can store the sample buffer at room temperature.
Find additional tips, troubleshooting help, and resources within our Protein Gel 1D Electrophoresis Support Center.
Can I use the PowerEase Touch 120W Power Supply for transferring gels with the SureLock Tandem Midi Blot Module, Mini Gel Blot Module, or Novex Semi-Dry Blotter?
No, most transfer applications require high current and therefore, we recommend using a high current power supply like PowerEase Touch 350W Power Supply.
Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.
Can I run Bio-Rad gels in the Mini Gel Tank?
No. Bio-Rad gels are of a different size (length, width, and depth) and have a special feature that precludes them from fitting in the Mini Gel Tank.
Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.