The green fluorescent Alexa Fluor™ 488 ovalbumin conjugate is a protein with a relatively low molecular weight (∼45,000 daltons) labeled with the bright, photostable and pH-insensitive Alexa Fluor™ 488 dye.
For Research Use Only. Not for use in diagnostic procedures.
仕様
標識タイプAlexa Fluor Dyes
製品ラインAlexa Fluor
タンパク質サブタイプAlbumin Proteins
数量2 mg
出荷条件Room Temperature
コンジュゲートAlexa Fluor 488
形状Lyophilized
Unit SizeEach
組成および保存条件
Store in freezer (-5 to -30°C) and protect from light.
引用および参考文献 (14)
引用および参考文献
Abstract
Cytosolic delivery of membrane-impermeable molecules in dendritic cells using pH-responsive core-shell nanoparticles.
Authors:Hu Y, Litwin T, Nagaraja AR, Kwong B, Katz J, Watson N, Irvine DJ,
Journal:Nano Lett
PubMed ID:17887715
'Polycations that absorb protons in response to the acidification of endosomes can theoretically disrupt these vesicles via the "proton sponge" effect. To exploit this mechanism, we created nanoparticles with a segregated core-shell structure for efficient, noncytotoxic intracellular drug delivery. Cross-linked polymer nanoparticles were synthesized with a pH-responsive core and hydrophilic ... More
In vivo targeting of dendritic cells for activation of cellular immunity using vaccine carriers based on pH-responsive microparticles.
Authors:Kwon YJ, James E, Shastri N, Fréchet JM
Journal:Proc Natl Acad Sci U S A
PubMed ID:16344458
'Activating the immune system to trigger a specific response is a major challenge in vaccine development. In particular, activating sufficient cytotoxic T lymphocyte-mediated cellular immunity, which is crucial for the treatment of many diseases including cancer and AIDS, has proven to be especially challenging. In this study, antigens were encapsulated ... More
Distinct pathways of antigen uptake and intracellular routing in CD4 and CD8 T cell activation.
Authors:Burgdorf S, Kautz A, Böhnert V, Knolle PA, Kurts C
Journal:Science
PubMed ID:17463291
The mechanisms that allow antigen-presenting cells (APCs) to selectively present extracellular antigen to CD8+ effector T cells (cross-presentation) or to CD4+ T helper cells are not fully resolved. We demonstrated that APCs use distinct endocytosis mechanisms to simultaneously introduce soluble antigen into separate intracellular compartments, which were dedicated to presentation ... More
A microengraving method for rapid selection of single cells producing antigen-specific antibodies.
Authors:Love JC, Ronan JL, Grotenbreg GM, van der Veen AG, Ploegh HL
Journal:Nat Biotechnol
PubMed ID:16699501
Monoclonal antibodies that recognize specific antigens of interest are used as therapeutic agents and as tools for biomedical research. Discovering a single monoclonal antibody requires retrieval of an individual hybridoma from polyclonal mixtures of cells producing antibodies with a variety of specificities. The time required to isolate hybridomas by a ... More
Early innate and adaptive immune perturbations determine long-term severity of chronic virus and Mycobacterium tuberculosis coinfection.