当社独自のアミン反応性色素であるOregon Green 488カルボン酸、スクシンイミジルエステルのこの単一の5異性体調製とそのコンジュゲートは、フルオレセインと同様の緑色蛍光を持ちますが、光安定性が高くなります。さらに、Oregon Green 488色素は、フルオレセインよりもpKaが低く(蛍光セインではpKa = 4.7対6.4)、この明るい色素は生理学的範囲でpHの影響を受けにくいようになっています。
研究用にのみ使用できます。診断用には使用いただけません。
仕様
化学反応性アミン
標識または色素Oregon Green™ 488
製品タイプカルボン酸スクシンイミジルエステル
数量5 mg
反応性部分活性エステル、スクシンイミジルエステル
出荷条件室温
標識タイプ従来の色素
製品ラインOregon Green
Unit SizeEach
組成および保存条件
フリーザー(-5∼-30度)に保存し、遮光してください。
引用および参考文献 (54)
引用および参考文献
Abstract
Microinjected antibodies interfere with protein nucleocytoplasmic shuttling by distinct molecular mechanisms.
Authors:Marg A, Meyer T, Vigneron M, Vinkemeier U,
Journal:Cytometry A
PubMed ID:18773464
'The observation that some antibodies can enter the nucleus after their microinjection into the cytoplasm established the principle of protein nucleocytoplasmic shuttling. Here, we introduce the concept of stationary antibodies for studying nuclear transport, particularly of native proteins. Contrary to the aforementioned translocating immunoglobulins, stationary antibodies do not cross the ... More
Sensitive fluorescence polarization technique for rapid screening of alpha-synuclein oligomerization/fibrillization inhibitors.
Authors:Luk KC, Hyde EG, Trojanowski JQ, Lee VM,
Journal:Biochemistry
PubMed ID:17927212
'Parkinson''s disease (PD) is characterized by the accumulation of fibrillar alpha-synuclein (alpha-Syn) inclusions known as Lewy bodies (LBs) and Lewy neurites. Mutations in the alpha-Syn gene or extra copies thereof cause familial PD or dementia with LBs (DLB) in rare kindreds, but abnormal accumulations of wildtype alpha-Syn also are implicated ... More
The dystroglycan complex is necessary for stabilization of acetylcholine receptor clusters at neuromuscular junctions and formation of the synaptic basement membrane.
Authors:Jacobson C, Côté PD, Rossi SG, Rotundo RL, Carbonetto S
Journal:J Cell Biol
PubMed ID:11157973
'The dystrophin-associated protein (DAP) complex spans the sarcolemmal membrane linking the cytoskeleton to the basement membrane surrounding each myofiber. Defects in the DAP complex have been linked previously to a variety of muscular dystrophies. Other evidence points to a role for the DAP complex in formation of nerve-muscle synapses. We ... More
Larval Schistosoma mansoni excretory-secretory glycoproteins (ESPs) bind to hemocytes of Biomphalaria glabrata (Gastropoda) via surface carbohydrate binding receptors.
Authors:Johnston LA, Yoshino TP
Journal:J Parasitol
PubMed ID:11534642
'Flow cytometric analysis of circulating blood cells (hemocytes) of Biomphalaria glabrata, molluscan intermediate host of Schistosoma mansoni, revealed the presence of 2 overlapping hemocyte subpopulations, designated R1 and R2. R1 hemocytes are characterized by their smaller size, reduced granularity, and the presence of the BGH1 surface epitope, whereas R2 cells ... More
Early acidification of phagosomes containing Brucella suis is essential for intracellular survival in murine macrophages.
Authors:Porte F, Liautard JP, Köhler S
Journal:Infect Immun
PubMed ID:10417172
'Brucella suis is a facultative intracellular pathogen of mammals, residing in macrophage vacuoles. In this work, we studied the phagosomal environment of these bacteria in order to better understand the mechanisms allowing survival and multiplication of B. suis. Intraphagosomal pH in murine J774 cells was determined by measuring the fluorescence ... More