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Citations & References (16)
Invitrogen™
Pepstatin A, BODIPY™ FL Conjugate
蛍光顕微鏡による細胞内のカテプシンDのトラフィッキングの研究については、当社のBODIPY FLペプスタチンAをお試しください。この緑色蛍光プローブは、生細胞のリソソーム内のカテプシンDをin vitroで阻害します（IC50約50 nM）。この新しいプローブを用いた初期の研究では詳細を見る
| 製品番号（カタログ番号） | 数量 |
|---|---|
| P12271 | 25 μg |
製品番号（カタログ番号） P12271
価格（JPY）お問い合わせください ›
40,200
Online offer
Ends: 27-Mar-2026
57,500割引額 17,300 (30%)
Each
数量:
25 μg
蛍光顕微鏡による細胞内のカテプシンDのトラフィッキングの研究については、当社のBODIPY FLペプスタチンAをお試しください。この緑色蛍光プローブは、生細胞のリソソーム内のカテプシンDをin vitroで阻害します（IC50約50 nM）。この新しいプローブを用いた初期の研究では、エンドサイト性経路を介して生細胞のリソソソソームに輸送され、非標識ペプスタチン A によって競争的に置換される可能性があることが示されています。
研究用にのみ使用できます。診断用には使用いただけません。
仕様
製品ラインBODIPY
数量25 μg
推奨保存方法冷凍庫内で保管し（-5～-30℃）、遮光してください。
出荷条件室温
物理的フォームSolid
製品タイプペプスタチンA
Unit SizeEach
引用および参考文献 (16)
引用および参考文献
Abstract
Monitoring autophagy in Alzheimer's disease and related neurodegenerative diseases.
Journal:Methods Enzymol
PubMed ID:19216904
'This chapter describes detailed methods to monitor autophagy in neurodegenerative disorders, especially in Alzheimer''s disease. Strategies to assess the competence of autophagy-related mechanisms in disease states ideally incorporate analyses of human disease and control tissues, which may include brain, fibroblasts, or other peripheral cells, in addition to animal and cell
Autophagy induction and autophagosome clearance in neurons: relationship to autophagic pathology in Alzheimer's disease.
Journal:J Neurosci
PubMed ID:18596167
'Macroautophagy, a major pathway for organelle and protein turnover, has been implicated in the neurodegeneration of Alzheimer''s disease (AD). The basis for the profuse accumulation of autophagic vacuoles (AVs) in affected neurons of the AD brain, however, is unknown. In this study, we show that constitutive macroautophagy in primary cortical
Lysosomal proteolysis and autophagy require presenilin 1 and are disrupted by Alzheimer-related PS1 mutations.
Journal:Cell
PubMed ID:20541250
Macroautophagy is a lysosomal degradative pathway essential for neuron survival. Here, we show that macroautophagy requires the Alzheimer's disease (AD)-related protein presenilin-1 (PS1). In PS1 null blastocysts, neurons from mice hypomorphic for PS1 or conditionally depleted of PS1, substrate proteolysis and autophagosome clearance during macroautophagy are prevented as a result
Targeted delivery of antigen processing inhibitors to antigen presenting cells via mannose receptors.
Journal:ACS Chem Biol
PubMed ID:20349916
Improved chemical inhibitors are required to dissect the role of specific antigen processing enzymes and to complement genetic models. In this study we explore the in vitro and in vivo properties of a novel class of targeted inhibitor of aspartic proteinases, in which pepstatin is coupled to mannosylated albumin (MPC6),
NAADP, cADPR and IP3 all release Ca2+ from the endoplasmic reticulum and an acidic store in the secretory granule area.
Journal:J Cell Sci
PubMed ID:16410548
Inositol trisphosphate and cyclic ADP-ribose release Ca2+ from the endoplasmic reticulum via inositol trisphosphate and ryanodine receptors, respectively. By contrast, nicotinic acid adenine dinucleotide phosphate may activate a novel Ca2+ channel in an acid compartment. We show, in two-photon permeabilized pancreatic acinar cells, that the three messengers tested could each