Pro-Q™ Emerald 488 Glycoprotein Gel and Blot Stain Kit
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Invitrogen™

Pro-Q™ Emerald 488 Glycoprotein Gel and Blot Stain Kit

Pro-Q エメラルド 488 糖タンパク質染色剤 は、ゲルおよびブロット中の糖タンパク質を直接検出します。ゲル染色は迅速かつ高感度です。Pro-Q エメラルド糖タンパク質染色剤は過ヨウ素酸化糖鎖と反応し、糖タンパク質上に明るい緑色蛍光シグナルを生じます詳細を見る
製品番号(カタログ番号)数量
P218751 kit
製品番号(カタログ番号) P21875
価格(JPY)
69,800
Each
お問い合わせください ›
数量:
1 kit
一括またはカスタム形式をリクエストする
Pro-Q エメラルド 488 糖タンパク質染色剤 は、ゲルおよびブロット中の糖タンパク質を直接検出します。ゲル染色は迅速かつ高感度です。Pro-Q エメラルド糖タンパク質染色剤は過ヨウ素酸化糖鎖と反応し、糖タンパク質上に明るい緑色蛍光シグナルを生じます。染色手順に必要なのは、固定、酸化、染色—の3つのステップのみです。Pro-Q エメラルド488 染色剤は、グリコシル化の性質と程度に応じて、ゲル中のバンドあたりわずか4 ngの糖タンパク質を検出できるため、酸性のフクシン色素を使用した標準的な過ヨウ素酸–シッフ塩基法よりも約50倍感度が高くなります。シグナルは、励起極大510 nmに近い波長の可視光で可視化できます。

すべての糖タンパク質染色剤を比較 ›

この染色剤は、一般的なタンパク質染色(SYPRO ルビーなど)と組み合わせることができ、ゲルおよびブロット中の糖タンパク質および総タンパク質を二色検出することができます。
研究用にのみ使用できます。診断用には使用いただけません。
仕様
検出位置ブロット内検出、ゲル内検出
検出法蛍光
製品ラインPro-Q
製品タイプ糖タンパク質染色キット
数量1 kit
品質保持期間6カ月
出荷条件室温
標的分子タンパク質(糖タンパク質)
標識または色素Pro-Q Emerald 488
Unit SizeEach

よくあるご質問(FAQ)

I have stained my blot with Pro-Q Emerald 300/488 Glycoprotein Blot Stain. Can I also stain the blot with SYPRO Ruby Blot Stain for total protein detection?

Yes. We recommend staining with SYPRO Ruby Blot Stain after staining with Pro-Q Emerald 300 or 488 Glycoprotein Blot Stain.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

引用および参考文献 (11)

引用および参考文献
Abstract
Proteomics identification of differentially expressed proteins associated with pollen germination and tube growth reveals characteristics of germinated Oryza sativa pollen.
Authors:Dai S, Chen T, Chong K, Xue Y, Liu S, Wang T,
Journal:Mol Cell Proteomics
PubMed ID:17132620
Mature pollen from most plant species is metabolically quiescent; however, after pollination, it germinates quickly and gives rise to a pollen tube to transport sperms into the embryo sac. Because methods for collecting a large amount of in vitro germinated pollen grains for transcriptomics and proteomics studies from model plants ... More
Unique posttranslational modifications of chitin-binding lectins of Entamoeba invadens cyst walls.
Authors:Van Dellen KL, Chatterjee A, Ratner DM, Magnelli PE, Cipollo JF, Steffen M, Robbins PW, Samuelson J,
Journal:Eukaryot Cell
PubMed ID:16682461
'Entamoeba histolytica, which causes amebic dysentery and liver abscesses, is spread via chitin-walled cysts. The most abundant protein in the cyst wall of Entamoeba invadens, a model for amebic encystation, is a lectin called EiJacob1. EiJacob1 has five tandemly arrayed, six-Cys chitin-binding domains separated by low-complexity Ser- and Thr-rich spacers. ... More
Glycoproteomics of paclitaxel resistance in human epithelial ovarian cancer cell lines: towards the identification of putative biomarkers.
Authors:Di Michele M, Marcone S, Cicchillitti L, Della Corte A, Ferlini C, Scambia G, Donati MB, Rotilio D,
Journal:J Proteomics
PubMed ID:19951750
'Glycosylation, one of the most common post translational modifications (PTMs) of proteins, is often associated with carcinogenesis and tumor malignancy. Ovarian cancer is the sixth cause of cancer-related death in Western countries. Currently, it is treated by debulking surgery followed by chemotherapy based on paclitaxel, alone or in combination with ... More
Protein expression and fucosylated glycans of the serum haptoglobin-{beta} subunit in hepatitis B virus-based liver diseases.
Authors:Shu H, Zhang S, Kang X, Li S, Qin X, Sun C, Lu H, Liu Y,
Journal:Acta Biochim Biophys Sin (Shanghai)
PubMed ID:21606158
'Glycosylation, which regulates the configuration and function of glycoproteins, is the most important post-translational modification. The aim of this study was to observe the differential patterns in glycan and protein parts of the serum haptoglobin-ß subunit (Hp-ß) purified from patients with hepatitis B virus (HBV) infection, liver cirrhosis (LC), or ... More
The Clostridium difficile exosporium cysteine (CdeC)-rich protein is required for exosporium morphogenesis and coat assembly.
Authors:Barra-Carrasco J, Olguín-Araneda V, Plaza-Garrido A, Miranda-Cárdenas C, Cofré-Araneda G, Pizarro-Guajardo M, Sarker MR, Paredes-Sabja D,
Journal:
PubMed ID:23794627
Clostridium difficile is an important nosocomial pathogen that has become a major cause of antibiotic-associated diarrhea. There is a general consensus that C. difficile spores play an important role in C. difficile pathogenesis, contributing to infection, persistence, and transmission. Evidence has demonstrated that C. difficile spores have an outermost layer, ... More